Bioassay-Guided Isolation of Anti-InflammatoryC-Glucosylflavones fromPassiflora edulis

Abstract

Passiflora edulis, commonly known as "maracujá", is widely cultivated in Brazil for the industrial production of juice. The species of Passiflora are popularly used as a sedative or tranquillizer, and also against intermittent fever and skin inflammation. In this study we evaluated the anti-inflammatory activity of four sub-fractions and three isolated compounds from the butanolic fraction of P. edulis var. flavicarpa leaves, using the mouse model of pleurisy induced by carrageenan. The butanolic fraction obtained from an aqueous extract of P. edulis (50 and 100 mg/kg, I. P.) showed anti-inflammatory activity by inhibiting leukocytes and neutrophils (p < 0.01). Sub-fraction C showed itself to be more effective than the other sub-fractions (p < 0.01). Isoorientin ( 1), vicenin-2 ( 2) and spinosin ( 3) were isolated from the active sub-fraction C derived from the butanolic fraction. The sub-fraction C (50 mg/kg, I. P.), as well as its major isolated compounds (25 mg/kg, I. P.), inhibited leukocytes and neutrophils (p < 0.05). Additionally, the butanolic fraction and isoorientin also inhibited myeloperoxidase activity (p < 0.05). The present study showed that the C-glucosylflavones isolated from P. edulis leaves can be responsible for the anti-inflammatory effect of P. edulis on the mouse model of pleurisy.

Effects of Passiflora edulis on the Metabolic Profile of Diabetic Wistar Rat Offspring

“…43 P. incarnata reportedly contains glycosyl flavonoids, 44,45 and P. edulis has antioxidant 46,47 and anti-inflammatory properties. 48,49 These properties may explain its effects on glycemia and the lipid profile in the animals we studied. The antioxidant compounds of P. edulis are 16 apigenin or luteolin derivatives, which include 4 mono-C-glycosyl, 8 O-glycosyl-C-glycosyl, and 4 O-glycosyl derivatives.…”

A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: Application to chemical variability evaluation

“…A recent review on the chemical constituents of the Lychnophorinae tribe has revealed significant variation in the occurrence of secondary metabolites in the genus Lychnophora [12]. Considering a single species, three phenomena can account for the observed differences: (a) accomplishment of a phytochemical investigation looking for a specific class of target secondary metabolites only, such as sesquiterpene lactones [13,14]; (b) performance of bioguided fractionation, which inspects a typical biological activity and may result in the isolation of a common class of secondary metabolites [15][16][17]; and (c) natural chemical variations in metabolism or presence of chemotypes [6][7][8]10].…”

Avaliação da atividade antiúlcera e segurança de uso de Passiflora setacea D.C (Passifloraceae) e Passiflora tenuifila Killip (Passifloraceae)

“…ZUCOLOTTO e colaboradores, isolaram iso-orientina, vicenina-2 e spinosina da fração butanólica do extrato de Passiflora edulis, e atribuíram parte da atividade anti-inflamatória a estes compostos (ZUCOLOTTO et al, 2009 morte precoce e à outras patologias advindas da presença de patógenos no solo quando comparada às demais espécies tradicionalmente cultivadas (MENEZES et al, 1994, OLIVEIRA et al,1994, MELETTI e BRUCKNER, 2001, FISCHER, 2003 …”

“…ZUCOLOTTO e colaboradores, isolaram iso-orientina, vicenina-2 e spinosina da fração butanólica do extrato de Passiflora edulis, e atribuíram parte da atividade anti-inflamatória a estes compostos (ZUCOLOTTO et al, 2009 Passiflora setacea é uma espécie de maracujá bastante utilizada no preparo de doces e sorvetes e é denominada popularmente por maracujá-sururuca, maracujá-do-sono, maracujá de cascavel (OLIVEIRA e RUGGIERO 2005). Porém esta espécie é pouco estudada, com quase nenhuma informação sobre sua composição química e efeitos medicinais.…”

“…A atividade anti-inflamatória de extratos de Passiflora edulis e Passiflora alata foi bastante estudada (BENINCA et al, 2007;MONTANHER et al, 2007;VARGAS et al, 2007;ZUCOLOTTO et al, 2009)…”