Bioassay of Pregnant Mare Serum Gonadotrophin by the Chick-32p Method

Abstract

Data are presented illustrating the reliability of the chick-32P method for the assay of pregnant mare serum gonadotrophin (PMS). Experiments with treatments of 1.25 IU PMS to 20.00 IU PMS demonstrated the advantages of this assay method. The testis-32P uptake provided a more sensitive end-point as a measure of gonadotrophin activity than did gonad weight change.

‘Believe Me Ever Most Truly and Affectly Yours’: John Robinson and Charles Jenkinson: Friendship and Sentiment within and without the Corridors of Power

“…While this was being resolved, Robinson busied himself with a 'very deserving, meritorious object', the advancement of the curate of Harwich, a 'poor man with a large family', 156 who conscientiously ministered to a flock long since deserted by its debt-ridden vicar -another of Robinson's embarrassing Wordsworth relatives. 157 Jenkinson obliged with a duchy of Lancaster living in nearby Dedham, and Robinson reported the curate's gratitude, adding, 'I am happy to hear good Accounts of your Lordship and all your family.' 158 No further letters survive.…”

The effects of changes in thyroid function on testis 32P uptake and the response to gonadotropin in the chick

“…and counted immediately in a Nuclear Chicago liquid scintillation counter. Differences in isotope uptake levels implied changes in testicular metabolism because of the demonstrated incorporation of V into the various chemical fractions and subcellular components of the chick testis and the general distribution of the isotope throughout the metabolic pool of phosphorus (Connell, 1964).…”

Effect of Hypophysectomy on the Radioactive Phosphorus Uptake of Chick Adrenals

“…T HE rate of incorporation of radioactive phosphorus ( 32 P) into the target organs of young chickens serves as an end-point in the bioassay procedures for thyrotropic, gonadotropic and adrenocorticotropic hormones (Greenspan et al, 1956;Brenemanetal., 1965;Connell, 1965;Bukovsan, 1967). The tropic hormones stimulate an increased incorporation of radioactivity per unit weight of their specific target organ.…”