Background: One of the ideal properties of a root canal sealer is to have a good sealing ability, especially at the apical third of the root. Objective: To evaluate the comparison of the apical leakage between obturation using bioceramic sealer (BS) and polydimethylsiloxane sealer (PS). Materials and Methods: Thirty-six mandibular premolars were equally divided into two groups and were obturated with single cone technique. The sealers used for Group I and Group II were BS and PS respectively. After obturation, the samples were incubated (37˚C, 24 h), sealed with two coats of nail polish except for 2 mm from the apex, submerged in the Indian ink for 7 days, decalcified, dehydrated, and made transparent according to Robertson technique. Dye penetration was evaluated under stereomicroscope. Samples without dye penetration were given score 0, dye penetration ≤ 0.5 mm were given score 1, 0.51 -1 mm were given score 2, and >1 mm were given score 3. Result: The largest proportion distribution in BS group was at the score 1 (55.6%), whereas in PS group was at the score 2 (44.4%). Conclusion: Bioceramic sealer showed similar apical leakage to polydimethylsiloxane sealer. Clinical Relevance: Based on this study, bioceramic sealer can be recommended to be used as sealer with low level of apical leakage as well as polydimethylsiloxane sealer.