Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions

Elbashiti, Tarek; Hamamcı, Haluk; Öktem, Hüseyin Avni; Yücel, Meral

doi:10.1016/j.plantsci.2005.02.024

Cited by 84 publications

(49 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Numerous studies have correlated trehalose with tolerance to abiotic stresses in plants, particularly drought (Paul et al, 2008). El-Bashiti et al (2005) showed that trehalose may play a role as an osmoprotectant compound in wheat species under salt and drought stress conditions. Zhang et al (2006) demonstrated that transgenic sugarcane transformed with the Grifola frondosa trehalose synthase (TSase) gene accumulated high levels of trehalose and showed enhanced tolerance to drought.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in drought-tolerant sugarcane roots

et al. 2015

View full text Add to dashboard Cite

ABSTRACT. Drought is one of the most frequent abiotic stresses limiting the productivity and geographical distribution of sugarcane culture. The use of drought-tolerant genotypes is one approach for overcoming the effects of water stress. We conducted a comparative study to identify gene expression profiles under water stress in tolerant sugarcane roots. Two different cultivars, 1 drought tolerant (RB867515) and 1 drought susceptible (SP86-155), were evaluated at 4 sampling time points (1, 3, 5, and 10 days) using the cDNA-amplified fragment length polymorphism technique. A total of 173 fragments were found to be differentially expressed in response to water stress in the tolerant cultivar. Seventy of these were cloned, sequenced, and categorized. Similarity analysis using Candidate genes to drought tolerance in sugarcane BLAST revealed that 64% of the fragments differentially expressed code proteins classified as no hits (23%), hypothetical (21%), or involved in stress response (20%), with others were involved in communication pathways and signal transduction, bioenergetics, secondary metabolism, and growth and development. Four genes were analyzed and validated using real-time quantitative polymerase chain reaction to determine their expression and showed consistency with the cDNA-amplified fragment length polymorphism analyses. Our results contribute insight into the molecular responses to water stress in sugarcane and possibility to the development of cultivars with improved tolerance to drought.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential gene expression in drought-tolerant sugarcane roots

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Plants exposed to high salt levels must cope with the dual challenges of ion toxicity and osmotic stress. Several species have been observed to accumulate trehalose in response to salt stress, including wheat (El-Bashiti et al, 2005), rice (Shima et al, 2007), and Medicago truncatula (López et al, 2008). However, overall levels of trehalose in the salt-stressed plants were still relatively low, suggesting that trehalose made little direct contribution to osmoregulation, unless its distribution within the plants was concentrated in localized tissues or subcellular compartments.…”

Section: Tre6p and Abiotic Stress Tolerancementioning

confidence: 99%

“…Trehalose accumulation in response to water stress in wheat was associated with a decrease in trehalase activity (El-Bashiti et al, 2005;Kosmas et al, 2006). However, trehalose levels were still relatively low and unlikely to make much contribution to osmoregulation unless very localized.…”

Section: Tre6p and Abiotic Stress Tolerancementioning

confidence: 99%

A Tale of Two Sugars: Trehalose 6-Phosphate and Sucrose

2016

View full text Add to dashboard Cite

“…As in dicotyledonous plants, overexpression of genes for the pathway has produced benefits such as stress tolerance in rice (Garg et al, 2002;Ge et al, 2008). Of the little that has been documented on the trehalose pathway in wheat, active enzymes are present in roots and shoots (El-Bashiti et al, 2005) and transcripts in roots (Mohammadi et al, 2007) and the transcripts of TPS and TPP enzymes in wheat grain (Weichert et al, 2010). SnRK1 plays an important role in the regulation of metabolism and development in crop sinks such as potato (Solanum tuberosum) tubers (McKibbin et al, 2006) and in seeds of legumes (Weber et al, 2005;Radchuk et al, 2010).…”

mentioning

confidence: 99%

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

et al. 2011

View full text Add to dashboard Cite

Trehalose 6-phosphate (T6P) is a sugar signal that regulates metabolism, growth, and development and inhibits the central regulatory SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11). To better understand the mechanism in wheat (Triticum aestivum) grain, we analyze T6P content and SnRK1 activities. T6P levels changed 178-fold 1 to 45 d after anthesis (DAA), correlating with sucrose content. T6P ranged from 78 nmol g 21 fresh weight (FW) pregrain filling, around 100-fold higher than previously reported in plants, to 0.4 nmol g 21 FW during the desiccation stage. In contrast, maximum SnRK1 activity changed only 3-fold but was inhibited strongly by T6P in vitro. To assess SnRK1 activity in vivo, homologs of SnRK1 marker genes in the wheat transcriptome were identified using Wheat Estimated Transcript Server. SnRK1-induced and -repressed marker genes were expressed differently pregrain filling compared to grain filling consistent with changes in T6P. To investigate this further maternal and filial tissues were compared pre-(7 DAA) and during grain filling (17 DAA). Strikingly, in vitro SnRK1 activity was similar in all tissues in contrast to large changes in tissue distribution of T6P. At 7 DAA T6P was 49 to 119 nmol g 21 FW in filial and maternal tissues sufficient to inhibit SnRK1; at 17 DAA T6P accumulation was almost exclusively endospermal (43 nmol g 21 FW) with 0.6 to 0.8 nmol T6P g 21 FW in embryo and pericarp. The data show a correlation between T6P and sucrose overall that belies a marked effect of tissue type and developmental stage on T6P content, consistent with tissuespecific regulation of SnRK1 by T6P in wheat grain.

show abstract

Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions

Cited by 84 publications

References 30 publications

Differential gene expression in drought-tolerant sugarcane roots

Differential gene expression in drought-tolerant sugarcane roots

A Tale of Two Sugars: Trehalose 6-Phosphate and Sucrose

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

Contact Info

Product

Resources

About