Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity

Zaoti, Zannati Ferdous; Hasan, Sharizal; Hossain, Md. Firose; Hasan, Md. Faruk; Islam, Md. Asadul; Khalekuzzaman, Md.; Sikdar, Biswanath

doi:10.9734/mrji/2017/38206

“…PCR and sequencing was accomplished by 16S rRNA gene amplification for molecular detection of the isolated bacterial strains. Wang et al (2015) found an identity of 99% to Serratia marcescens strain CPO1 by the BLAST search. Similar result was also reported previously by Altschul et al (1990).…”

Section: Discussionmentioning

confidence: 97%

“…The two isolated bacterial strains were observed through several morphological tests. Gram staining and motility of bacteria was conducted as previously described ( Zaoti et al ., 2018 ).…”

Section: Methodsmentioning

confidence: 99%

“…Serratia marcescens is an ecologically and genetically diverse pathogenic bacterial species belonging to the family of Enterobacteriaceae (Besler & Little, 2017). S. marcescens may be present as a contamination on the surface of plants or fruits and enter the tissue via natural pores or sores (Wang et al, 2015). Previous reports showed that this bacteria is an opportunist pathogen that causes diseases in humans, such as cystitis (Liu et al, 2004), conjunctivitis (Hume & Willox, 2004) and others.…”

Section: Introductionmentioning

confidence: 99%

“…Serratia marcescens has been reported as a plant pathogen causing yellow vine disease in Citrullus lanatus and Cucurbita pepo (Sikora et al, 2012), soft rot in Capsicum annuum (Gillis et al, 2014), Zea mays whorl rot (Wang et al, 2015), yellow wilt on Helianthus (Ignatov et al, 2016), leaf rot in Cannabis sativa (Schappe et al, 2020) and others. Naturally active plant products are important source of eco-friendly bactericides to control different pathogenic bacteria in vitro and in vivo (Rahman et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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First report of Serratia marcescens associated with black rot of Citrus sinensis fruit, and evaluation of its biological control measures in Bangladesh

Hasan

¹

,

Islam

²

,

Sikdar

³

2020

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Background: The present study was designed to isolate and identify the phyto-pathogen responsible for black rot of Citrus sinensis, and to determine its biological control measures. Methods: The pathogen was isolated from infected oranges and cultured on Luria-Bertani medium. Gram staining method was used to identify the morphological characteristics of the causal agents of the black rot. Advanced molecular technique was applied to facilitate proper detection of the isolated bacteria. Phylogenetic trees were analyzed using the Neighbor-Joining method. Antimicrobial screening was conducted by disc diffusion method. Antagonistic activity was evaluated by well diffusion method. Results: Gram staining of the causal agent showed rod shaped, small and pink bacteria. Polymerase chain reaction of the 16S ribosomal RNA gene amplified an approximately 1465 bp product. The nucleotide sequences of the isolated bacterial sample 1 (BS1) and bacterial sample 2 (BS2) had 99.34% and 99.45% similarities with the reference of Serratia marcescens sequence in NCBI GenBank. The obtained sequences were deposited in GenBank. Two isolates showed virulence capability on some fresh fruits, which confirmed the stain detection and Koch’s postulates. Allium sativum extract showed the largest (27.33±1.5 mm) diameter of zone of inhibition against BS1, at 30µg/disc concentration. In the antagonistic assay, Rhizobium leguminosarum showed largest (19±1 mm) zone of inhibition against BS1. Conclusions: Findings of the current investigations are constructive for identification of causative pathogens in Citrus sinensis black rot disease and their biological control measures.

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“…Amplification of the 16S rRNA gene was performed by conventional PCR technique using the universal primers: 27F forward primer 5'-TGGTAGTCCACGCCCTAAAC-3' and 1492R reverse primer 5'-GACGGGCGGTGTGTRCA-3' [15][16] in a 25µL reaction volume, containing nuclease free ddH 2 O 15μL, dNTP mix 1.0μL, forward primer 1.0μL, reverse primer 1.0μL, DNA template 1.5μL, MgCl 2 2.5μL, Taq buffer B 2.5μL, and Taq polymerase 0.5μL. The PCR was done by initial denaturation at 95°C for 5 min; 35 cycles of denaturation at 95°C for 40s, annealing at 65°C for 1 min, extension at 72°C for 2 min and the final extension at 72°C for 10 min, followed by cooling at 4°C until the sample was taken out.…”

Section: Pcr Analysismentioning

confidence: 99%

Molecular Detection and Biological Control of Human Hair Dandruff Causing Microorganism Staphylococcus aureus

Mas-ud¹,

Dna²,

Ali³

et al. 2020

J. Pure Appl. Microbiol.

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Human hair dandruff (HHD) is a common unwanted scalp disorder that is prevalent to most human populations all over the world. This study was designed to isolate and characterize pathogens that are responsible for HHD as well as the evaluation of their biological control technique. Isolated bacteria were characterized by different biochemical tests and molecular identification methods. Here, disc diffusion methods were used to determine antibiotic and antibacterial activity against isolated bacteria. The isolated bacterial colonies were found to be Gram-positive, small, round-shaped, and purple. PCR amplification was done using 27F and 1492R primer pairs. A BlastN search of a sequenced 1465 bp region of 16S rRNA in NCBI GenBank revealed approximately 99% genome similarity with Staphylococcus aureus. The sequence was deposited in GenBank (Accession No. MH603394). In the antibiotic sensitivity test, Kanamycin showed the highest 31.0±0.5 mm diameter zone of inhibition (DZI) against the isolated bacteria. Moreover, as a plant-derived compound, the Methanol extract of Allium sativum revealed the highest, 15.0±0.5 mm DZI. The present study would give a promising direction of identification and control of this pathogen biologically.

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First report of Serratia marcescens associated with black rot of Citrus sinensis fruit, and evaluation of its biological control measures in Bangladesh

Hasan

¹

,

Islam

²

,

Sikdar

³

2022

Self Cite

View full text Add to dashboard Cite

Background: The present study was designed to isolate and identify the phyto-pathogen responsible for black rot of Citrus sinensis, and to determine its biological control measures. Methods: The pathogen was isolated from infected oranges and cultured on Luria-Bertani medium. Gram staining method was used to identify the morphological characteristics of the causal agents of the black rot. Advanced molecular technique was applied to facilitate proper detection of the isolated bacteria. Phylogenetic trees were analyzed using the Neighbor-Joining method. Antimicrobial screening was conducted by disc diffusion method. Antagonistic activity was evaluated by well diffusion method. Results: Gram staining of the causal agent showed rod shaped, small and pink bacteria. Polymerase chain reaction of the 16S ribosomal RNA gene amplified an approximately 1465 bp product. The nucleotide sequences of the isolated bacterial sample 1 (BS1) and bacterial sample 2 (BS2) had 99.34% and 99.45% similarities with the reference of Serratia marcescens sequence in NCBI GenBank. The obtained sequences were deposited in GenBank. Two isolates showed virulence capability on some fresh fruits, which confirmed the stain detection and Koch’s postulates. Allium sativum extract showed the largest (27.33±1.5 mm) diameter of zone of inhibition against BS1, at 30µg/disc concentration. In the antagonistic assay, Rhizobium leguminosarum showed largest (19±1 mm) zone of inhibition against BS1. Conclusions: Findings of the current investigations are constructive for identification of causative pathogens in Citrus sinensis black rot disease and their biological control measures.

show abstract

Biochemical and Molecular Characterization of Bacterial Wilt Disease of Banana and Evaluation of their Antibiotic Sensitivity

Cited by 4 publications

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First report of Serratia marcescens associated with black rot of Citrus sinensis fruit, and evaluation of its biological control measures in Bangladesh

First report of Serratia marcescens associated with black rot of Citrus sinensis fruit, and evaluation of its biological control measures in Bangladesh

Molecular Detection and Biological Control of Human Hair Dandruff Causing Microorganism Staphylococcus aureus

First report of Serratia marcescens associated with black rot of Citrus sinensis fruit, and evaluation of its biological control measures in Bangladesh

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