Biochemical characterization and molecular evidence of a laccase from the bird’s nest fungus Cyathus bulleri

Vasdev, Kavita; Dhawan, Sanju; Kapoor, Rajeev Kumar; Kuhad, Ramesh Chander

doi:10.1016/j.fgb.2005.03.013

Cited by 51 publications

(32 citation statements)

References 46 publications

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“…Non-denaturing gel electrophoresis was performed under the same conditions except that SDS and β-mercaptoethanol were not used, and samples were not boiled. Laccase activity in native gel electrophoresis was determined by soaking gel in 100 mM citrate-phosphate buffer (pH 5.0) containing 0.25% guaiacol [36]. The appearance of dark brown band indicated the presence of laccase.…”

Section: Gel Electrophoresis and Zymogram Analysismentioning

confidence: 99%

Banana Peel: A Potential Substrate for Laccase Production by Aspergillus fumigatus VkJ2.4.5 in Solid-State Fermentation

Vivekanand

Dwivedi

Pareek

et al. 2011

Appl Biochem Biotechnol

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In solid-state fermentation, among various solid supports evaluated, banana peel was found to be an ideal support and resulted into higher levels of laccase (6281.4 ± 63.60 U l(-1)) along with notable levels of manganese peroxidase production (1339.0 ± 131.23 U l(-1)) by Aspergillus fumigatus VkJ2.4.5. Maximum levels of laccase was achieved under derived conditions consisting of 80% of moisture level, 6 days of incubation period, 6% inoculum level, and an aeration level of 2.5 l min(-1). A column-tray bioreactor was designed to scale up and economize the enzyme production in three successive cycles of fermentation using the same fungal biomass. Thermal and pH stability profiles revealed that enzyme was stable up to 50°C and at varying pH range from 5-9 for up to 2 h. The apparent molecular weight of laccase was found to be 34 ± 1 kDa. MALDI-TOF/TOF analysis of the protein showed significant homology with maximum identity of 67% to other laccases reported in database.

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Section: Gel Electrophoresis and Zymogram Analysismentioning

confidence: 99%

Banana Peel: A Potential Substrate for Laccase Production by Aspergillus fumigatus VkJ2.4.5 in Solid-State Fermentation

Vivekanand

Dwivedi

Pareek

et al. 2011

Appl Biochem Biotechnol

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“…It has been widely reported that laccases have quite different catalytic activities under different incubation conditions. 8,11,17,19 The laccase from R. lignosus W1 exhibited favourable activity at acidic pH and had good stability above pH 4. Although no laccase activity was detected at alkaline pH, the laccase activity increased during storage at alkaline pH.…”

Section: Discussionmentioning

confidence: 99%

“…The appearance of dark brown bands due to polymerisation of guaiacol indicated the presence of laccase. 17 The molecular mass of the laccase involved in decolorisation was evaluated by separation of crude proteins by 12% sodium dodecyl sulfate (SDS) PAGE along with a standard protein marker. 18 Activity staining following SDS-PAGE was carried out by incubating the gel in 2 g L −1 Triton X-100 in sodium citrate buffer (pH 4.5) 19 and staining with RBBR for decolorisation followed by guaiacol to detect the enzyme activity.…”

Section: Electrophoresis and Stainingmentioning

confidence: 99%

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Dai

Peng

et al. 2008

J of Chemical Tech & Biotech

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BACKGROUND: Effluents from the dyeing process containing dyes are difficult to degrade biologically. Therefore enzymatic dye decolorisation has received considerable attention recently. In this study the dye decolorisation potential of crude laccase from the white rot fungus Rigidoporus lignosus W1 was demonstrated on an anthraquinone dye, Remazol Brilliant Blue R (RBBR), and a triphenylmethane dye, malachite green (MG). Effects of pH, temperature and ionic strength on laccase activity and decolorisation efficiency were investigated.

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“…KX879638 and Lentinus spp. KY006984 were maintained on 2% (g/L) malt extract agar (MEA) and incubated at 25°C for 8 to 10 days (Vasdev et al, 2005). Primary screening of the isolates was performed on 2% (w/v) malt extract agar plate containing 0.01% guaicol (Kiiskinen et al, 2004).…”

Section: Screening Of Fungal Isolates For Laccase Activitymentioning

confidence: 99%

Effect of surfactants on lignin modifying enzyme activity of Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation

Singh¹,

Singh²

2017

Afr. J. Microbiol. Res.

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Lignin modifying enzyme (LME) activity [laccase, lignin peroxidase (LiP) and manganese peroxidase (MnP)] was studied in two indigenously isolated white rot fungi, Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation (SSF) of wheat bran and saw dust supplemented with various non-ionic polysorbate surfactants (Tweens) and Triton X-100. Both fungal species proved to be highly ligninolytic and promising laccase producers. Their laccase activity increased many folds due to supplementation of substrates with surfactants. Among surfactants, Tween 20 was the best, giving 39180 IU/g laccase in Ganoderma spp. and 37780 IU/g in Lentinus spp. on wheat bran corresponding to 3.8-and 10.6-fold increase over control condition. Triton X-100 was the best surfactant providing 29380 and 19877 IU/g laccase activity in Ganoderma spp. and Lentinus spp. on saw dust amounting to 2.9-and 3.9-fold increase over control condition, respectively. Ganoderma spp. always produced more laccase than Lentinus spp. on supplemented substrates. Between substrates, laccase production on supplementation was always more on wheat bran than saw dust. LiP and MnP activities were very low in comparison with laccase in both fungi under control conditions (1.92 to 4.07 IU/g and 0.98 to 2.07 IU/g, respectively) and upon treatment with surfactants; they did not show an appreciable change, thus, supplementation mediated ratio between laccase to LiP/MnP was larger.

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Biochemical characterization and molecular evidence of a laccase from the bird’s nest fungus Cyathus bulleri

Cited by 51 publications

References 46 publications

Banana Peel: A Potential Substrate for Laccase Production by Aspergillus fumigatus VkJ2.4.5 in Solid-State Fermentation

Banana Peel: A Potential Substrate for Laccase Production by Aspergillus fumigatus VkJ2.4.5 in Solid-State Fermentation

Decolorisation of synthetic dyes by crude laccase from Rigidoporus lignosus W1

Effect of surfactants on lignin modifying enzyme activity of Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation

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