Biochemical characterization of embryogenic and non-embryogenic calluses of sugarcane

Neves, Nadina; Segura-Nieto, Magdalena; Blanco, M.; Sánchez, Margelys; González, Augusto; González, José Ignacio López; Castillo, R

doi:10.1079/ivp2002391

Cited by 43 publications

(32 citation statements)

References 26 publications

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“…The balance among free and conjugated PA forms may be critical in different developmental processes (Torrigiani et al 1987), and when the conjugated PA levels are high, organogenesis proceeds (Scaramagli et al 1999). On the other hand, high levels of Put at the beginning of somatic embryogenesis are related to the ability of competent cells to develop somatic embryos (Nieves et al 2003). Taken together, results for PAs obtained in the present study revealed that synthesis and accumulation of these compounds occurred at the onset of somatic embryogenesis.…”

Section: Resultssupporting

confidence: 73%

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

Cangahuala-Inocente

Silveira

Caprestano

et al. 2013

In Vitro Cell.Dev.Biol.-Plant

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Feijoa (Acca sellowiana [O. Berg] Burret [Myrtaceae]) is a native fruit species of southern Brazil and northern Uruguay. This species is amenable to somatic embryogenesis and therefore suitable as a model system for comparative studies of zygotic and somatic embryo development. In seed plants, embryogenesis involves three main steps, which are regulated by many factors, such as hormones, proteins, polyamines, and transcription factors. In the present work, the dynamics of protein, sugar, starch, amino acid, and polyamine accumulation were assayed during somatic embryogenesis of A. sellowiana . Protein, starch, amino acid, and polyamine levels accumulated unevenly during the induction phase of somatic embryogenesis, while the sugar content remained stable. Throughout the different developmental stages of somatic embryogenesis, synthesis and accumulation of proteins and amino acids showed patterns similar to those reported previously during the development of zygotic embryos of this same species. Differential patterns of polyamine accumulation were observed. This is important because these compounds affect the synthesis of other endogenous growth regulators, such as auxinindole-3-acetic acid that is mainly involved in the establishment of embryo polarity. Taken together, the present work brings new insights to the physiological and biochemical dynamics that occur during somatic embryogenesis of A. sellowiana.

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Section: Resultssupporting

confidence: 73%

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

Cangahuala-Inocente

Silveira

Caprestano

et al. 2013

In Vitro Cell.Dev.Biol.-Plant

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“…These enzymes catalyze a variety of reactions, including the oxidation of various organic and inorganic compounds in the presence of peroxides, such as hydrogen peroxide. Peroxidase activity in the plants is related to the regulation of growth and differentiation processes, such as the inhibition of growth by regulating the cell wall lignification; morphogenic changes in response to physical, chemical and biological stress; wound healing; pathogen defense; the regulation of cell elongation; and other characteristics (Kao 2003;Nieves et al 2003;Piza et al 2003;Campos et al 2004;Maciel 2007;Freitas et al 2008). Other biochemical components considered are the contents of total and reducing sugars, amino acids and soluble proteins.…”

Section: Introductionmentioning

confidence: 99%

Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis

Marques

Freitas

Guedes

et al. 2013

Braz. arch. biol. technol.

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The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed nodetectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively.

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“…Vários autores relatam que calos embriogênicos de cana-de-açúcar são formados com a adição de auxinas sintéticas no meio de cultura (HO; VASIL, 1983;TAYLOR;DUKIC, 1993;GANDONOU et al, 2005;LAKSHMANAN, 2006), dentre eles estão o Dicamba (GANDONOU et al, 2005;GAJ, 2004;JIMÉNEZ, 2005), Picloran (CHENGALRAYAN; GALLO-MEAGHER, 2005;GARCIA et al, 2007) e 2,4-D (BLANCO et al, 1997;NIEVES et al, 2003;GANDONOU et al, 2005;LAKSHMANAN et al, 2006;WATT et al, 2009). Porém, a manutenção prolongada e os repetidos subcultivos podem causar uma instabilidade genética (GILL; MALHTRA; GOSAL, 2006) e aumentar a probabilidade de variação somaclonal (BURNER; GRISHAM, 1995;FRANKLIN et al, 2006;LAKSHMANAN et al, 2006), afetando o potencial embriogênico em canade-açúcar.…”

Section: Introductionunclassified

Embriogênese somática da cultivar RB966928 e do clone RB986419 de cana-de-açúcar (Saccharum spp.)

et al. 2013

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ResumoA cana-de-açúcar é uma cultura muito plantada devido a sua grande importância econômica, provenientes de seus produtos, etanol e açúcar. O Brasil é o maior produtor e exportador mundial de açúcar, sendo assim a cultura está freqüentemente inserida em programas de melhoramento genético. A transformação genética é estratégia para obtenção de cultivares resistentes e produtivas. Para obtenção de plantas transgênicas são necessários protocolos definidos e otimizados de regeneração in vitro. O objetivo desse trabalho foi avaliar a influência de diferentes concentrações de 2,4-D (ácido 2,4 diclorofenoxiacético) para obtenção de calos embriogênicos na cultivar RB966928 e no clone RB986419. Folhas imaturas, de plantas com 10 meses de idade, foram utilizadas como fonte de explantes. Cilindros foliares de 8 x 150 mm, segmentados em 4 mm, foram inoculados em meio de cultura MS com cinco concentrações de 2,4-D (1, 3, 9, 16 e 27 µM).Um segundo experimento foi realizado com as concentrações de 3, 9 e 16 µM de 2,4-D. Para os dois experimentos, a desdiferenciação do material ocorreu no escuro. Os calos foram transferidos após 45 dias de subcultivo para novo meio de cultura, com as mesmas concentrações de origem para sua multiplicação. A cultivar RB966928 teve respostas de embriogênese somática de 30 e 41% utilizando 16 µM de 2,4-D para o primeiro e segundo experimentos, respectivamente. O clone RB986419 apresentou, respectivamente, 26 e 80% de formação de calos embriogênicos, utilizando-se 9 µM de 2,4-D no primeiro e segundo experimento. Os calos formados foram transferidos para meio MS sem regulador de crescimento vegetal. As plântulas formadas foram aclimatizadas e colocadas em casa de vegetação. As melhores concentrações de 2,4-D para a formação de calos embriogênicos foram 16 µM para a cv. RB966928 e 9 µM para o clone RB986419. Palavras-chave: Calos embriogênicos, 2,4-D, cultivo in vitro AbstractThe sugar cane is a crop widely planted because of the economic importance of its products, sugar and ethanol. Brazil is the largest producer and exporter of sugar, so the crop is often inserted in breeding programs. Genetic transformation strategy is used to obtain resistant and productive cultivars. Defined and optimized in vitro regeneration protocols are required to obtain transgenic plants. The aim of this study was to evaluate the influence of different concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) to obtain embryogenic callus on cultivars RB966928 and RB986419 clone. Immature leaves from

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Biochemical characterization of embryogenic and non-embryogenic calluses of sugarcane

Cited by 43 publications

References 26 publications

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

Dynamics of physiological and biochemical changes during somatic embryogenesis of Acca sellowiana

Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis

Embriogênese somática da cultivar RB966928 e do clone RB986419 de cana-de-açúcar (Saccharum spp.)

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