Biochemical effects of sodium benzoate, potassium sorbate and sodium nitrite on food spoilage yeast Saccharomyces cerevisiae

Yardımcı, Berna Kavakcıoğlu; Cengiz, Sevilay; Sever, Nurettin İlter; Özek, Nihal Şimşek

doi:10.1007/s11756-021-00964-x

Cited by 10 publications

(7 citation statements)

References 42 publications

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“…To reduce the food safety risk caused by A. flavus , some synthetic preservatives have been widely used to prevent food infection and spoilage for many years [6, 7]. However, the use of chemical preservatives in foods brings new problems, such as drug resistance, toxicity, and drug interactions [8], which makes it urgent to seek alternative, safe and effective antifungal agents to replace the currently used in food industry.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination

Yao

Meng

2022

J Basic Microbiol

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Phenyllactic acid (PLA) has gained a lot of attention due to its broad antimicrobial activity, but the mechanism of its antifungal action has been barely reported until now. Herein, the inhibitory activity of PLA against Aspergillus flavus spore germination and its mechanism were preliminarily investigated. Results indicated that PLA had a strong antifungal activity against A. flavus with the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of 6 and 12 mg/ml, respectively. As observed by scanning electron microscopy (SEM), the A. flavus spores displayed wrinkled and shrunken appearance after treatment with PLA. In addition, the permeability and integrity of A. flavus cell membrane were changed obviously after PLA treatment as indicated by the propidium iodide (PI) staining results, which was further confirmed by a rise in electric conductivity and increased leakage of intracellular protein and nucleic acid. Furthermore, reduced activities of mitochondrial ATPase and dehydrogenases caused by PLA were also observed in A. flavus spores, with a result of remarkable decrease in ATP synthesis. Therefore, it could be concluded that PLA was effective in inhibiting spore germination of A. flavus mainly by disrupting cell membrane and interfering with mitochondrial energy metabolism.

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Section: Introductionmentioning

confidence: 99%

Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination

Yao

Meng

2022

J Basic Microbiol

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“…Sodium benzoate has been reported to as useful agent with antimicrobial properties that able to inhibit the growth of many microbes, including Pseudomonas aeruginosa (Schroeter) Migula [ 36 ], Penicillium commune Thom [ 37 ], E. coli , Staphylococcus aureus Rosenbach, Saccharomyces cerevisiae Meyen ex E.C. Hansen [ 38 ], and Candida albicans (Robin) Berkhout [ 39 ], indicating the possibility that the greater tolerance toward sodium benzoate caused osmotic stress may account for the environmental fitness of B. dothidea . Additionally, the comparative genomics analysis between B. dothidea and the other three Botryosphaeria species found 143 B. dothidea species-specific genes, and further analysis of the above species-specific genes via the KEGG pathways found jg10977 has a predicated role in the calcium permeable stress-gated cation channel.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genomic Difference among Four Botryosphaeria Pathogens in Chinese Hickory Trunk Canker

Ma¹,

Yan²,

Zhang³

2023

JoF

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Botryosphaeria species are amongst the most widespread and important canker and dieback pathogens of trees worldwide, with B. dothidea as one of the most common Botryosphaeria species. However, the information related to the widespread incidence and aggressiveness of B. dothidea among various Botryosphaeria species causing trunk cankers is still poorly investigated. In this study, the metabolic phenotypic diversity and genomic differences of four Chinese hickory canker-related Botryosphaeria pathogens, including B. dothidea, B. qingyuanensis, B. fabicerciana, and B. corticis, were systematically studied to address the competitive fitness of B. dothidea. Large-scale screening of physiologic traits using a phenotypic MicroArray/OmniLog system (PMs) found B. dothidea has a broader spectrum of nitrogen source and greater tolerance toward osmotic pressure (sodium benzoate) and alkali stress among Botryosphaeria species. Moreover, the annotation of B. dothidea species-specific genomic information via a comparative genomics analysis found 143 B. dothidea species-specific genes that not only provides crucial cues in the prediction of B. dothidea species-specific function but also give a basis for the development of a B. dothidea molecular identification method. A species-specific primer set Bd_11F/Bd_11R has been designed based on the sequence of B. dothidea species-specific gene jg11 for the accurate identification of B. dothidea in disease diagnoses. Overall, this study deepens the understanding in the widespread incidence and aggressiveness of B. dothidea among various Botryosphaeria species, providing valuable clues to assist in trunk cankers management.

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“…The fact that the biochemical makeup of the cell alters depends on the viability and vitality changes induced by various exogenous factors makes it important to determine these alterations [43]. In this paper, we have used FTIR microspectroscopy as an economic, rapid, and non-destructive technique to reveal the major biochemical changes associated with entrapment of the yeast cells in PHEMA-NVF cryogels [44].…”

Section: Ftir Analysis Of Cellular Damage Markers In Yeast Cells Rele...mentioning

confidence: 99%

Evaluation of the antiproliferative and oxidative effects of polymeric cryogels on the model eukaryotic yeast Saccharomyces cerevisiae

Yardımcı,

Şarkaya,

Güler

2024

Preprint

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Here, the effects of previously characterized poly(HEMA-N-vinyl formamide) (PHEMA-NVF) cryogels on viability, surface morphology, total antioxidant-oxidant status, antioxidant enzyme activities and biochemical makeup of Saccharomyces cerevisiae were investigated for the first time. The viability of the cells released from the PHEMA-NVF cryogels were found to decrease dramatically up to 23.96 ± 1.44% compared to the plain PHEMA cryogel as control group without NVF. Additionally, this cryogel caused blebbing and holes on the cell surface. Although TOS levels gradually increased with the increasing amounts of NVF, statistically significant difference was determined only between the cryogel containing 22 µl NVF and control. The gradual increases in TAS levels in response to cellular stress were recorded depend on the increase of NVF amounts up to 22 µl. While enzymatic antioxidant system was generally induced in cells released from the cryogel prepared with relatively low levels of NVF, these activities dramatically decreased at the higher amounts of the comonomer. Finally, FTIR analysis showed that especially the highest studied concentration of NVF caused protein degradation and increase the relative concentration of lipid and polysaccharide molecules. Therefore, it can be suggested that this comonomer causes oxidative stress that the cell cannot cope with above a certain amount.

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Biochemical effects of sodium benzoate, potassium sorbate and sodium nitrite on food spoilage yeast Saccharomyces cerevisiae

Cited by 10 publications

References 42 publications

Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination

Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination

Phenotypic and Genomic Difference among Four Botryosphaeria Pathogens in Chinese Hickory Trunk Canker

Evaluation of the antiproliferative and oxidative effects of polymeric cryogels on the model eukaryotic yeast Saccharomyces cerevisiae

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