Biochemical properties of a new thermo- and solvent-stable xylanase recovered using three phase partitioning from the extract of Bacillus oceanisediminis strain SJ3

Abstract

The present study investigates the production and partial biochemical characterization of an extracellular thermostable xylanase from the Bacillus oceanisediminis strain SJ3 newly recovered from Algerian soil using three phase partitioning (TPP). The maximum xylanase activity recorded after 2 days of incubation at 37 °C was 20.24 U/ml in the presence of oat spelt xylan. The results indicated that the enzyme recovered in the middle phase of TPP system using the optimum parameters were determined as 50% ammonium sulfate saturation with 1.0:1.5 ratio of crude extract: t-butanol at pH and temperature of 8.0 and 10 °C, respectively. The xylanase was recovered with 3.48 purification fold and 107% activity recovery. The enzyme was optimally active at pH 7.0 and was stable over a broad pH range of 5.0–10. The optimum temperature for xylanase activity was 55 °C and the half-life time at this temperature was of 6 h. At this time point the enzyme retained 50% of its activity after incubation for 2 h at 95 °C. The crude enzyme resist to sodium dodecyl sulfate and β-mercaptoethanol, while all the tested ions do not affect the activity of the enzyme. The recovered enzyme is, at least, stable in tested organic solvents except in propanol where a reduction of 46.5% was observed. Further, the stability of the xylanase was higher in hydrophobic solvents where a maximum stability was observed with cyclohexane. These properties make this enzyme to be highly thermostable and may be suggested as a potential candidate for application in some industrial processes. To the best of our knowledge, this is the first report of xylanase activity and recoverey using three phase partitioning from B. oceanisediminis.

Three-Step Purification and Characterization of Organic Solvent-Tolerant and Alkali-Thermo-Tolerant Xylanase from Bacillus paramycoides T4 [MN370035]

“…The ability to withstand organic solvents could be a paramount property of xylanases as several industrial and biotechnological applications are carried out in the presence of solvents. For instance, ethanol-tolerant xylanases are employed in bioethanol production; solvent-tolerant xylanases are used in the bioremediation of solvent-contaminated industrial wastewaters; solvent-and surfactant-tolerant xylanases are used in the deinking of recycled paper; solvent tolerance makes it easier to selectively precipitate, recover, and use enzymes [55][56][57][58], as well as in the bioconversion of lignocellulose [59]. The xylanase under the current study revealed significant stability with hydrophobic (log p > 2.0) and hydrophilic (log p < 2.0) organic solvents.…”

Isolation and Characterization of Human Intestinal Bacteria Cytobacillus oceanisediminis NB2 for Probiotic Potential

“…Biochemical, physiological, and taxonomic characterization identifies it as a species of Bacillus . Members of the Bacillus were characterized by diverse habitats, including human feces, and showed a wide range of biotechnological potential (Singh et al, 2009 ; Zhang et al, 2010 ; Kumar et al, 2013 , 2014 ; Patel et al, 2014 ; Boucherba et al, 2017 ). Various Bacillus species have already been characterized for probiotic potential (Elshaghabee et al, 2017 ).…”

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

“…(Kumar S et al, 2017). Bacillus oceanisediminis SJ3, também isolado de solo, manteve altas taxas enzimáticas de 48 h até 72 h (Boucherba N et al, 2017). Contudo, distintos tempos de incubação têm sido reportados para a máxima produção de xilanases: 24 h para Geobacillus stearothermophilus KIBGE-IB29 (Bibi Z et al, 2014), 56 h para Bacillus pumilus VLK-1 (Kumar L et al, 2014), 96 h para Arthrobacter sp.MTCC 6915 (Murugan S et al, 2011) e Bacillus mojavensis AG137 (Sepahy AA, Ghazi S, Sepahy MA, 2011).…”