Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts

Abstract

RESUMO.-[Respostas bioquímicas, termográficas e foliculares de modelos murinos tratados hormonalmente após xenotransplante de ovário bovino sob a cápsula renal.] Este estudo teve como objetivo avaliar as características dos dois diferentes modelos de murinas tratadas hormonalmente após xenotransplante de tecido ovariano bovino sob a cápsula renal. Dois modelos de camundongos imunodeficientes (BALB/c NUDE e C57BL6 SCID) receberam fragmentos de ovário de novilhas e cada grupo foi submetido a dois tratamentos hormonais de eCG ou uma combinação de FSH+LH. This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey's test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.

Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models

“…Recipients were anesthetized with 2.5% 2,2,2-tribromoethanol at the dose of 18 ml kg -1 (SCID) and 20 ml kg -1 (NUDE) by intraperitoneal administration. Dosages were established from previous experiments ( Pimentel et al, 2017 ).…”

Use of Thermography in Reconstructive Surgery Associated With Laser Therapy—Review of Literature

“…Another complication of the technique is the size of tissue is important. A small size cannot show differences of temperature in different fragments, but the thermography can help monitoring the conditions of a larger tissue or organ, including transport and transplant (Pimentel et al, 2017).…”

“…In the attempt to improve the healing process of complications suffered by the flaps or even avoid them, several techniques have been studied. These techniques aim to reduce inflammation, promote neovascularization, and improve tissue repair, such as Lithospermum officinale (Amiri et al, 2017), platelet rich plasma (Pazzini et al, 2016) and phototherapies, which include the low-power laser and LED (Corazza & Jorge, 2007).…”

Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft

“…Ovarian xenotransplantation is used as an alternative method to maintain ovarian functions such as hormone release, ovulation, and fertility through the development of ovarian tissue of a particular species in a specific site of another species. Therefore, the technique is valuable for patients submitted to chemotherapy or irradiation therapy that had their reproductive functions compromised, animals with economical and ecological relevance and also for preservation of endangered species (Kikuchi et al 2011, Pimentel et al 2017. Considering that bovine slaughterhouse ovaries are abundantly available and that the species has similarities with humans in reproductive physiology, bovine ovarian xenotransplantation is a promise procedure (Langbeen et al 2016).…”

“…Considering that the hematological and biochemical parameters help to assess possible organism pathologies or functional changes, the analysis of these parameter profiles can be used to evaluate the ovarian xenotransplantation technique in relation to damages caused in the function of the recipient site and the homeostasis of the animal during the experimental period (Pimentel et al 2017). Currently, data on hematological and biochemical analysis that can be correlated to this study are scarce in the literature.…”