Biocidal Activity of two botanical volatile oils against the larvae of Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae)

Abstract

The volatile oils of golden pillar, Cupressus macrocarpa and galangal, Alpinia officinarum were extracted and their constituents were identified. Alpha pinene and trans-caryophyllene were the major isolated constituents of C. macrocarpa oil, and 1,8 cineole and 4-terpeniol were the major compounds of A. officinarum oil.The present study revealed that the botanical volatile oils used, had morphogenic effects against S. nudiseta stages. These include larval-pupal intermediates, pupal-adult intermediates, deformed adults with crumpled wings and/ or deformed thorax and abdomen. Also some adults couldn't emerge and remained in their puparia.The histological effects induced by oils treatment in the body wall were represented by degeneration and detachment of hypodermis and dissolving of the exocuticle in some regions. The histopathological effects that appeared in the midgut were shrinkage in some epithelial cells and swelling of other cells.

Peruvian Botanical Biopesticides for Sustainable Development and Protection of the Environment

“…The morphological aberrations obtained are similar to those described on Synthesiomyia nudiseta Wulp larvae (Khalaf et al, 2009;Khalil, Assae, Abo El Mahasen, & Mahmoud, 2010) and on M. domestica (Elkattan, Ahmed, Elbermaway, & Abdel-Gawad, 2011), causing a 24-h delay in the development of the larvae treated with an extract of seeds in comparison to other treatments. The effect of delay during the transformation from larvae to pupae was also described by other authors (Elkattan et al, 2011;Ande, 2001;Assar & Abo-Shaeshae, 2004).…”

“…This means that the equality of the means was rejected. Therefore, the means obtained must be transformed by applying the square root of the arccosine and then by related to the effect of inhibiting the fly growth process by substances known as IGRs, a consideration that has been supported by other authors (Khalaf et al, 2009;Youssef, 1997;Wang, Li, & Lei, 2005).…”

“…The housefly, Musca domestica L. (Diptera: Muscidae), is considered a vector of viruses, bacteria, and protozoa that cause many diseases in humans and other mammals and has become an important public health concern (Urzúa et al, 2010a;Sripongpun, 2008). Management of M. domestica has been carried out through the application and extensive use of synthetic insecticides, which in turn have caused serious environmental problems, such as the development of pest resistance, ecological damage, and irreversible damage to human health (Vásquez, de Baptista, Trevizan, & Gadanha, 2008;Ahmed, Zain, & Irfanullah, 2004;Nivsarkar, Cherian, & Padh, 2001; Kristensen & Jespersen, 2003;Taşkin, Kence, & Göçmen, 2004;Khalaf, Hussein, & Shoukry, 2009).…”

“…A vast number of studies conducted worldwide have focused on the insect-control uses of insect growth regulators (IGRs) and plant secondary metabolites (Céspedes, Salazar, Martínez, & Aranda, 2005;Torres et al, 2003;Magalhães et al, 2010;Pohlit, Rezende, Lopes, Lopes, & Neto, 2011;Urzúa et al, 2010b). Plant-derived compounds that are rich sources of bioactive chemicals provide promising alternatives to the current use of chemical pesticides for insect control (Khalaf et al, 2009). The Asteraceae family constitutes an important source of secondary metabolites, such as monoterpenes, sesquiterpenes, sesquiterpene lactones, diterpenes, triterpenes, flavonoids, coumarins, polyacetylenes, and benzofurans (Raal et al, 2011;Alvarenga, Ferreira, Emerenciano & Cabrol-Bass, 2001;Ferreira, Brant, Alvarenga, & Emerenciano, 2005).…”

Influence of Some Natural Substances for Control the Greater Wax Moth Galleria mellonella l. (Lepidoptera: Pyralidae)

“…Plants may provide potential alternatives to currently used insectcontrol agents because plants constitutions were considered as rich source of bioactive chemicals (Wink, 1993). Additionally, plant-derived materials are found to be highly effective against insecticide resistant insect pests (Kwon et al, 1996), so that many investigators initiated a large screening efforts poisonous effects to use them as insecticides (El-Shazly and Hussien, 2004;Prowse et al, 2006;Malarvannan andSubashini, 2007 andKhalaf et al, 2009).…”