Biocompatibility of a new biodegradable polymer-hydroxyapatite composite for biomedical applications

Macha, Innocent J.; Ben‐Nissan, Besim; Santos, Jerran; Cazalbou, Sophie; Stamboulis, Artemis; Grossin, David; Giordano, Gérard

doi:10.1016/j.jddst.2017.01.008

Cited by 39 publications

(20 citation statements)

References 16 publications

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“…The chemical structure and surface properties of PTLG‐PNIPAAm were investigated by Fourier transform infrared spectroscopy, gel permeation chromatography, X‐ray photoelectron spectrum, scanning electron microscope, and water contact angle. HADSCs 21–23which were considered as a kind of ideal seed cell for translational and regenerative medicine research were cultured on PTLG‐PNIPAAm surface at 37 °C, and then the cell sheet detached from the surface spontaneously at temperature below PNIPAAm LCST. For the surface phase transition behavior of PTLG‐PNIPAAm, a type as the “hook and loop” process was proposed to understand the cell adhesion and detachment behavior on modified material surface.…”

Section: Introductionsupporting

confidence: 93%

Surface Modification of PTLG Terpolymer Using PNIPAAm and Its Cell Adhesion/Detachment Behavior

Gong

Liu

Fan

et al. 2020

Macro Chemistry & Physics

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Novel biodegradable terpolymer poly (1,3‐trimethylene carbonate‐b‐(l‐lactide‐r‐glycolide)) grafted with poly(N‐isopropylacrylamide) (PTLG‐PNIPAAm) is prepared by plasma‐induced polymerization. The experimental results show that PNIPAAm segments of samples have a dramatic effect on surface layer structure and cell behavior of PTLG‐PNIPAAm. The thickness of PNIPAAm layer on PTLG surface increases rapidly from 50 nm to 1 µm with the molecular weight of samples increasing. Cell toxicity analysis indicates that PTLG‐PNIPAAm possesses good cytocompatibility to human adipose‐derived stem cells (hADSCs). For cell adhesion and detachment process on modified surface, the “hook and loop” process is proposed to understand the cell adhesion on modified surface with coiled conformation PNIPAAm segments at 37 °C, and then the cell sheet detached from surface spontaneously due to the conformation of PNIPAAm segments extends below its lower critical solution temperature. The research demonstrates that with increasing thickness of the PNIPAAm layer on PTLG surface, the hADSCs with well cell viability presents rapider thermal‐responsive detachment behavior. Therefore, the surface modification of biodegradable PTLG with stimuli‐sensitive functional PNIPAAm is an efficacious route for further application in stem cell culture and tissue engineering stent.

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Section: Introductionsupporting

confidence: 93%

Surface Modification of PTLG Terpolymer Using PNIPAAm and Its Cell Adhesion/Detachment Behavior

Gong

Liu

Fan

et al. 2020

Macro Chemistry & Physics

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“…In addition, the cell density was increased with 2.5 and 5% by weight of HAp, as it can be observed in the respective images, then it can be confirmed that HAp promotes cell adhesion and cell viability to scaffolds. This could be related to its chemical structure, which contains functional groups such as calcium phosphates and hydroxyls that would be interacting with functional groups from osteoblasts groups . These facts can be supported by the work of Jeong and his collaborators who studied the cytoskeleton organization of the cells bound in three‐dimensional scaffolds by fluorescent staining of actin filaments of the adhered cells up to 24 h in vitro cultures.…”

Section: Resultsmentioning

confidence: 93%

“…Nonetheless, the hydrophobicity of some materials, like PLA, may limit the cell adhesion during tissue regeneration process . Therefore, the incorporation of bioactive ceramic materials such as hydroxyapatite (HAp) and tricalcium phosphate (TCP) has represented an option to be used for polymeric composite scaffolds, because they would favor reactions with physiological fluids, due to its chemical nature and similarity to the native bone …”

Section: Introductionmentioning

confidence: 99%

“…Regarding the chemical environment of the scaffolds, the hydrophobicity of the polymeric materials could limit the process of cell adhesion. The incorporation of bioactive ceramic materials such as HAp into polymeric materials may favor reactions with physiological fluids . Also, the surface modification with peptides like arginine‐glycine‐aspartic (RGD) that stimulate the integrins, has been reported.…”

Section: Introductionmentioning

confidence: 99%

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Influence of RGD Peptide on Morphology and Biocompatibility of 3D Scaffolds Based on PLA/Hydroxyapatite

et al. 2019

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The developments of three‐dimensional and bioactive supports are essential for bone tissue engineering. Therefore, this study reports the morphology and biological behavior of osteoblast cells grown on bioactive 3D scaffolds, obtained by electro‐spinning and based on poly(lactic acid), PLA, and Hydroxyapatite, HAp, composites (PLA/HAp), which were superficially treated with RGD. Likewise, the characterization of the obtained 3D scaffolds is presented and discussed. The results indicate that it is possible to obtain structures with porosities higher than 90% with interconnected channels, which favor cell adhesion and growth. Also, the biological performance evaluation of the scaffolds indicates that surface treatment with RGD was effective and has a synergistic effect along with HAp. The cell adhesion was also observed by SEM and the images show cells extended and adhered to the scaffold's fibers along with neighboring cells. Also, the HAp had an influence on cell proliferation, which showed the maximum values with 2.5 and 5.0% wt of HAp. Therefore, 3D PLA/HAp scaffolds, obtained by electrospinning, treated with RGD represent promising material for use in bone tissue engineering.

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“…Hydroxyapatite (HA) is well known as a bioactive ceramic added to many artificial bone tissues and implants for orthopedic surgeries [14][15][16]. Not only polymer/HA composites exhibit the chemical and mechanical stability of natural bones [17,18], but also they can be designed in the form of porous materials that accelerate the circulation of essential biochemical agents such as VD 3 [19]. Given the significant chemical (e.g.…”

Section: Introductionmentioning

confidence: 99%

Effect of HA Nanoparticles on Adsorption of Vitamin D3 on Super-Hydrophobic PA6 Nanofibrous Scaffold

Esfahani

Ghiyasi

2020

Matéria (Rio J.)

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Vitamin D 3 has significant roles in bone growth and the prevention of osteoporotic fractures. The present study investigated the effect of hydroxyapatite nanoparticles decoration onto polyamide-6 nanofibrous scaffold on adsorption behaviour of Vitamin D 3. To synthesize the nanofibrous scaffold, an electrospinning device was used, and the surface of the scaffold was characterized by scanning electron microscopy, energy dispersive spectrometry, Fourier transform infrared spectroscopy, and measurement of the water contact angle. The antibacterial activity test against E. coli and S. aureus bacteria indicated no such activity of pristine and hydroxyapatite decorated scaffold. The results demonstrated that a hydrophobic and high porous scaffold was formed, and hydroxyapatite nanoparticles were distributed inside the polyamide-6 nanofibers homogenously. Results showed that the hydroxyapatite nanoparticles improved the adsorption efficiency of polyamide-6 scaffold. It was found that the amount of adsorbed Vitamin D 3 molecules onto the polyamide-6/HA scaffold was rapid during the first hour of immersion (24.4 ng.cm-3), then declined over the next 3 h, and eventually reached a stable percentage of about 10.3 ng.cm-3. This phenomenon appears to be related to the high adsorption potential of porosities and the hydrophobic nanofibers during the first stage of immersion and non-occupied hydroxyapatite ceramic sites during the final stage of immersion.

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Biocompatibility of a new biodegradable polymer-hydroxyapatite composite for biomedical applications

Cited by 39 publications

References 16 publications

Surface Modification of PTLG Terpolymer Using PNIPAAm and Its Cell Adhesion/Detachment Behavior

Surface Modification of PTLG Terpolymer Using PNIPAAm and Its Cell Adhesion/Detachment Behavior

Influence of RGD Peptide on Morphology and Biocompatibility of 3D Scaffolds Based on PLA/Hydroxyapatite

Effect of HA Nanoparticles on Adsorption of Vitamin D3 on Super-Hydrophobic PA6 Nanofibrous Scaffold

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