Biocompatibility of Yttria-Tetragonal Zirconia Polycrystal Seeded with Human Adipose Derived Mesenchymal Stem Cell

Abstract

Introduction:The scaffold is a place for regeneration of new bone and bone tissue growths in tissue engineering applications. hADMSC is a multipotent cell which can differentiate into osteogenic, chondrogenic and adipogenic. Y-TZP has been shown to have several advantages over other ceramics because of its hard nature, namely fracture toughness and high flexural strength.Aim:This study aimed to analyze the biocompatibility of Y-TZP as a scaffold seeded with hADMSCs by in vitro analysis.Material and methods:This research involved several processes, namely Y-TZPS manufacture process, XRD examination, differentiation and characterization of hADMSC, SEM observation, and then TT.Results:The results of the XRD examination showed that Y-TZPSs had sharp peaks. It suggests that they had high crystal purity. The marked expression of the characterization of hADMSC is the positive expression of Cluster of differentiation (CD), namely CD 90, CD 73 and CD 105 above NMT and negative expressions of CD 14, CD 19, CD 34, CD 45 and also HLA-DR below NLT. The analysis of observations on the Y-TZPSs with SEM, subsequently, indicated the porosity of Y-TZPSs, as a result, the adhesion of HADMSCs occurred and grew in the porosity in the Y-TZPSs.Conclusions:Y-TZPSs with low porosity and toxicity can be able to proliferate and differentiate if seeded with hADMSC. Y-TZPSs are expected to be used as implantable biomaterials using hADMSCs with high biocompatibility.

Alveolar Repair Using Cancellous Bone and Beta Tricalcium Phosphate Seeded With Adipose-Derived Stem Cell

“…Isolation and culture of hADSC were carried out until the fourth passage according to Banyard et al 25 differentiation was carried out according to Jeon et al, 26 showing adipogenic and osteogenic lineage, as previously mentioned in our earlier study. 27,28…”

“…MSC-specific surface markers suggested CD 90, CD 73, and CD 105 were strongly positive expressed above 95%, whereas CD 14, CD 19, CD 34, CD 45, and human leukocyte antigen (HLA)-DR were found to be negatively expressed below 2%, as previously mentioned in our earlier study. 27,28…”

“…Isolation and culture of hADSC were carried out until the fourth passage according to Banyard et al 25 differentiation was carried out according to Jeon et al, 26 showing adipogenic and osteogenic lineage, as previously mentioned in our earlier study. 27,28 Characterization of hADSC Characterization of hADSC was analyzed using BD LSR II Flow Cytometer (BD Biosciences). MSC-specific surface markers suggested CD 90, CD 73, and CD 105 were strongly positive expressed above 95%, whereas CD 14, CD 19, CD 34, CD 45, and human leukocyte antigen (HLA)-DR were found to be negatively expressed below 2%, as previously mentioned in our earlier study.…”

Design, fabrication, mechanical, and in vitro evaluation of 3D printed ZrO2 reinforced polylactide scaffolds through fused deposition modeling

“…25 Zirconia (ZrO 2 ) is generally preferred as an alternative to HAP in BTE due to its salient features of biocompatibility, high bending strength, fracture toughness, and Young's modulus. [26][27][28][29] Individual ZrO 2 ceramics are reported to display high mechanical strength than expected in certain areas of hard tissue replacement, and moreover, its inert characteristics show negligence to promote new bone formation at the defective site. 30,31 Moreover, the fabrication of ZrO 2 scaffolds through modern 3D printing techniques is laborious, and the attainment of desired scaffold design becomes a challenging task.…”