Biocompatible heterogeneous porous gel matrix NeuroGelTM promotes regeneration of rat sciatic nerve within tubular silicone prosthesis (experimental study)

Abstract

Neurogel™ strongly promotes the regeneration of rat sciatic nerve within silicone tubular prosthesis. After bridging a 10-mm gap through silicone prosthesis with Neurogel™ or Neurogel™ +NGF-B-modified intraluminal space, the myelination rate of regenerated axons of rat sciatic nerve appeared to be higher, and the axon count and functional recovery is similar to results seen with the autografting technique.

Effects of Pulsed Electromagnetic Fields on Peripheral Nerve Regeneration Using Allografts in Sciatic Nerve: An Animal Model Study

“…Although both morphological and functional data have been used to assess neural regeneration after induced crush injuries, the correlation between these two types of assessment is usually poor [18][19][20]. Classical and newly developed methods of assessing nerve recovery, including histomorphometry, retrograde transport of horseradish peroxidase and retrograde fluorescent labeling [20] do not necessarily predict the reestablishment of motor and sensory functions [19,[21][22][23]. Although such techniques are useful in studying the nerve regeneration process, they generally fail in assessing functional recovery [19].…”

Heterogeneous pHPMA hydrogel promotes neuronal differentiation of bone marrow derived stromal cells in vitro and in vivo

“…Recent experimental data suggest that transplantation of this hydrogel in modeling both acute and chronic SCIs leads to restoration of the damaged tissue structure, making possible the germination of cell processes of the recipient's tissue. Experimental transplantation has resulted in an increase in axonal growth cone numbers and new axons (including myelinated Schwann cells) in comparison with controls; 12 months after the operation, the structure of the hydrogel changes due to the outgrowth of blood vessels and cells inside the implant; 19 months after the operation, complete myelination is observed together with deposition of collagen and fibronectin fibers and synaptogenesis, including dendritic contact formation (Woerly 2000, Woerly et al 2004, 2005, Gatskiy et al 2014. Experimental animals showed functional recovery, including quadrupedal locomotion and restoration of descending innervation through the transplanted tissue.…”