Biocompatible Layers Obtained from Functionalized Iron Oxide Nanoparticles in Suspension

Abstract

Iron oxide nanoparticles have been extensively studied for challenges in applicable areas such as medicine, pharmacy, and the environment. The functionalization of iron oxide nanoparticles with dextran opens new prospects for application. Suspension characterization methods such as dynamic light scattering (DLS) and zeta potential (ZP) have allowed us to obtain information regarding the stability and hydrodynamic diameter of these suspended particles. For rigorous characterization of the suspension of dextran-coated iron oxide nanoparticles (D-MNPs), studies have been performed using ultrasound measurements. The results obtained from DLS and ZP studies were compared with those obtained from ultrasound measurements. The obtained results show a good stability of D-MNPs. A comparison between the D-MNP dimension obtained from transmission electron microscopy (TEM), X-ray diffraction (XRD), and DLS studies was also performed. A scanning electron spectroscopy (SEM) image of a surface D-MNP layer obtained from the stable suspension shows that the particles are spherical in shape. The topographies of the elemental maps of the D-MNP layer showed a uniform distribution of the constituent elements. The homogeneity of the layer was also observed. The morphology of the HeLa cells incubated for 24 and 48 h with the D-MNP suspension and D-MNP layers did not change relative to the morphology presented by the control cells. The cytotoxicity studies conducted at different time intervals have shown that a slight decrease in the HeLa cell viability after 48 h of incubation for both samples was observed.

Preparation and Characterization of Dextran Coated Iron Oxide Nanoparticles Thin Layers

“…The suspension synthesis and layer preparation were based on an adapted method presented previously [ 23 , 31 , 32 ]. Ferrous chloride tetrahydrate and chloride hexahydrate were mixed at room temperature and added drop by drop into the dextran solution (20% w/v ).…”

“…On the other hand, for the deposition of layers based on iron oxide / polymer, methods such as Matrix Assisted Pulsed Laser Evaporation (MAPLE), dip coating, spin coating, etc. could be used [ 23 , 24 ]. Encouraging results have been obtained by using SPION as a coating for medical devices due to the inhibition of microbial colonization induced by the presence of superparamagnetic iron oxide nanoparticles [ 25 ].…”

“…Moreover, a good biocompatibility with HepG2 cell line of dextran coated maghemite thin films was noticed [ 27 ]. Our previous studies reported the obtaining and characterization of iron oxide–dextran thin films on various substrates by various methods [ 23 , 24 , 27 , 28 ]. However, it is not yet fully understood how iron oxide nanoparticles interact with dextran and, on the other hand, further studies are needed to understand how the morphology of the layers influences the biological properties of the dextran coated iron oxides nanoparticles’ (DIO-NPs) thin layer.…”

Substituted hydroxyapatite coatings of bone implants

“…HAp doped with less than 1% of zinc ions has evidenced effective bioactivity and antibacterial properties. 87,88 Zn-HAp coatings have been fabricated by different techniques including plasma spraying, 89 EPD, 90 sol-gel spin coating 91 and magnetron sputtering. 92 The fabrication method determines the Zn distribution within the coating.…”

Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings

“…The toxicity of the HAp and 5SmHAp solutions and coatings was assessed using the HeLa cell line (Sigma-Aldrich Corp., St. Louis, MO, USA). The in vitro interaction of the HeLa cells with the samples was studied at three different time intervals (24,48, and 72 h) using the methodology previously described in Predoi et al [34]. A cell viability using live/dead cell double staining kit (purchased from Merck/Sigma-Aldrich) was also performed.…”