Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi

Deng, Jun‐Jin; Huang, Weixiong; Li, Zhiwei; Lu, De-Lin; Zhang, Yuanyuan; Luo, Xiao‐Chun

doi:10.1016/j.enzmictec.2018.02.002

Cited by 75 publications

(45 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…6). Of the up-regulated proteins, aspartic protease inhibits spore germination and growth of B. cinerea in addition to efficiently damaging the cell wall [31]. A putative esterase purified from Botrytis similarly showed that the protein is up-regulated during glucose depletion and mycelium deterioration, which is likely to signal advanced cell wall destabilization [32, 33].…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

Shi

Wang

et al. 2019

BMC Microbiol

View full text Add to dashboard Cite

BackgroundGrey mould is an important plant disease worldwide, caused by Botrytis cinerea, resulting in serious economic loss. Wuyiencin, a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic, has been demonstrated effectiveness against B. cinerea.ResultsWuyiencin treatment inhibited growth and sporulation of B. cinerea, specifically altering hypha morphology and intracellular structures. These changes were accompanied by differential expression (fold change > 2.0) of 316 proteins identified by iTRAQ-labelling LC-MS/MS analysis (P < 0.05). Up-regulation of 14 proteins, including carbohydrate metabolism proteins and cell wall stabilization proteins, was validated by parallel reaction monitoring (PRM). Down-regulation of 13 proteins was validated by PRM, including regulators of energy metabolism, nucleotide/protein synthesis, and the biosynthesis of mediators of plant stress and decay.ConclusionOur results confirm the inhibitory biological effects of wuyiencin on B. cinereal and elaborate on the differentially expressed proteins and associated pathways implicated in the capacity of wuyiencin to debilitate the growth and pathogenicity of grey mould. This study provides validated candidates for further targeted exploration with the goal of optimizing wuyiencin as a safe, low-toxicity agent for biological control.

show abstract

Section: Discussionmentioning

confidence: 99%

iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

Shi

Wang

et al. 2019

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…As shown in Table 1 Figure 6). Of the up-regulated proteins, aspartic protease inhibits spore germination and growth of B. cinerea in addition to efficiently damaging the cell wall [31]. A putative esterase purified from Botrytis similarly showed that the protein is up-regulated during glucose depletion and mycelium deterioration, which is likely to signal advanced cell wall destabilization [32,33].…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Proteomic Analysis Reveals the Mechanisms of Botrytis cinerea controlled with Wuyiencin

Shi

Wang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Grey mould is an important plant disease worldwide, caused by Botrytis cinerea ，resulting in serious economic loss. Wuyiencin, a low toxicity, high efficiency, and broad-spectrum agricultural antibiotic, has been demonstrated effectiveness against B. cinerea. Methods In this study, we characterized the differential protein expression in wuyiencin-treated B. cinerea using high-resolution mass spectrometry and bioinformatic tools. Results Wuyiencin treatment inhibited growth and sporulation of B. cinerea, specifically altering hypha morphology and intracellular structures. These changes were accompanied by differential expression (fold change >2.0) of 316 proteins identified by iTRAQ-labelling LC-MS/MS analysis (P < 0.05). Up-regulation of 14 proteins, including carbohydrate metabolism proteins and cell wall stabilization proteins, was validated by parallel reaction monitoring (PRM). Down-regulation of 13 proteins was validated by PRM, including regulators of energy metabolism, nucleotide/protein synthesis, and the biosynthesis of mediators of plant stress and decay. Conclusion Our results confirm the inhibitory biological effects of wuyiencin on B . cinereal and elaborate on the differentially expressed proteins and associated pathways implicated in the capacity of wuyiencin to debilitate the growth and pathogenicity of grey mould. This study provides validated candidates for further targeted exploration with the goal of optimizing wuyiencin as a safe, low-toxicity agent for biological control.

show abstract

“…Culturing temperature and water activity (aw) are also key factors. The maximum activity of protease P6281 produced by T. harzianum was observed at 40 • C [99]. The appropriate conditions for the growth of Kluyveromyces spp.…”

Section: Temperature and Water Activitymentioning

confidence: 99%

“…Regarding to Trichoderma species, only a few inhibitory compounds that play roles in their antagonistic interactions with aflatoxingenic fungi were reported. Deng et al [99] demonstrated that the aspartic protease P6281 secreted by T. harzianum could efficiently inhibit the conidial germination and the growth of A. flavus. Mostafaet al [43] demonstrated that T. harzianum and T. viride showed a high antagonism and inhibited aflatoxins production of A. flavus by 90%, which were explained partially by the liberation of extracellular enzymes and the production of inhibitory volatile compounds.…”

Section: Protease and Extracellular Enzymes Produced By Trichoderma Sppmentioning

confidence: 99%

Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors

Ren

Zhang

et al. 2020

Toxins

View full text Add to dashboard Cite

Aflatoxin contamination has been causing great concern worldwide due to the major economic impact on crop production and their toxicological effects to human and animals. Contamination can occur in the field, during transportation, and also in storage. Post-harvest contamination usually derives from the pre-harvest infection of aflatoxigenic molds, especially aflatoxin-producing Aspergilli such as Aspergillus flavus and A. parasiticus. Many strategies preventing aflatoxigenic molds from entering food and feed chains have been reported, among which biological control is becoming one of the most praised strategies. The objective of this article is to review the biocontrol strategy for inhibiting the growth of and aflatoxin production by aflatoxigenic fungi. This review focuses on comparing inhibitory behaviors of different antagonistic microorganisms including various bacteria, fungi and yeasts. We also reviewed the bioactive compounds produced by microorganisms and the mechanisms leading to inhibition. The key factors influencing antifungal activities of antagonists are also discussed in this review.

show abstract

Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi

Cited by 75 publications

References 32 publications

iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

iTRAQ-based proteomic analysis reveals the mechanisms of Botrytis cinerea controlled with Wuyiencin

iTRAQ-Based Proteomic Analysis Reveals the Mechanisms of Botrytis cinerea controlled with Wuyiencin

Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors

Contact Info

Product

Resources

About