Biocontrol of Late Blight Disease (Phytophthora capsici) of Pepper and the Plant Growth Promotion byPaenibacillus ehimensisKWN38

Abstract

For field application of a bacterial strain used to control Phythophthora capsici, we will need a biologically and economically efficient carrier medium. The known antagonist Paenibacillus ehimensis KWN38 was grown in a grass medium where it showed high antifungal and lytic enzyme activities. To demonstrate the potential of P. ehimensis KWN38 for biocontrol of late blight disease in pepper, pot trials were conducted by treating the 1-month-old plants with water (W), a selected grass medium (G3), G plus P. ehimensis KWN38 inoculation (G3P) or synthetic fungicide (F). The shoot dry weight in G3P was higher than that in W and F treatments at 15 days after zoospore infection (DZI). The root dry weight in G3P was also higher than that in W. The root mortality of G3 and W increased over 58 and 80% at 15 DZI, and some plants in those treatments wilted due to the failure of root physiology. The plants in G3P and F survived well because of their better root health conditions. Soil cellulase activity of G3P was consistently higher than that of W and F at earlier observation times (0, 2 and 6 DZI). The root b-1,3-glucanase activity of G3P promptly increased to maximum shortly after zoospore infection and reached the maximum value of 51.12 unit g À1 of fresh weight at 2 DZI. All these results indicate that inoculation of P. ehimensis KWN38 to the root zone of potted pepper plants increases plant growth, root and soil enzyme activities and alleviates the root death caused by infection with P. capsici zoospores.Biocontrol of pepper late blight disease K. W. Naing et al.

Effects of the Biocontrol Agent Aspergillus flavipes on the Soil Microflora and Soil Enzymes in the Rooting Zone of Pepper Plants Infected with Phytophthora capsici

“…These results agree with conclusion of Naing et al. (), who reported that soil enzyme activities were increased following inoculation of P. ehimensis KWN 38 and alleviated root death caused by infection with P. capsici zoopores.…”

“…Soil enzyme activities in rhizosphere soils of diseased plants were increased compared to healthy plants in the presence of ASD strain in the present study. These results agree with conclusion of Naing et al (2014), who reported that soil enzyme activities were increased following inoculation of P. ehimensis KWN 38 and alleviated root death caused by infection with P. capsici zoopores.…”

Isolation and Identification of Bacillus amyloliquefaciens IBFCBF‐1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper

“…(Virgen-Calleros et al 1997;Khabbaz et al 2015), Glomus intraradices (Zheng et al 2005), Aspergillus sp. (Kim et al 2009;Naing et al 2014). (Kim et al 2009;Naing et al 2014).…”

Induction of Resistance in Rice Against Magnaporthe Oryzae by Culture Filtrates of Biscogniauxia sp. O821

“…In rice, the blast fungus Magnaporthe oryzae is considered to be the most important cause of disease (Ou 1985). Similarly, Pseudomonas fluorescens isolates have been found to control pre-emergence damping-off disease (Nielsen et al 1998), whereas inoculation of the roots of pepper plants with Paenibacillus ehimensis KWN38 increases plant growth and may control Phytophthora capsici infection (Naing et al 2014). One of the most promising approaches for an effective biological control strategy is the use of microbes for plant disease management.…”