A high-yield mutant strain Rhizopus oryzae RL6041 induced hy ions implantation was used in the present study. L (-i-)-lactic acid was generated by RL6041 grown on liquefied corn starch as a substrate. With optimal conditions (Liquefied corn starch 150g/L, (NH4)2SO4 2.0g/L, MgSO4-7H2O l.Og/L, ZnSO4-7H2O 2g/L, KH2PO4 0.2g/L, CaCOg 8%, medium size 20/250M1, seed age 6h, fermentation temperature 38" C), the yield of L (+)-lactic acid in shake-flask reached 133~136g/L after 36h, indicating that the conversion rate was as high as 88%~91%, and the productivity was 3.75g/L-h. There was almost a 70% increase in lactic acid production compared with the original strain RE3303.