Biodegradable gelatin/silver nanoparticle composite cryogel with excellent antibacterial and antibiofilm activity and hemostasis for Pseudomonas aeruginosa-infected burn wound healing

Huang, Ying; Bai, Lang; Yang, Yanjing; Yin, Zhiwei; Guo, Baolin

doi:10.1016/j.jcis.2021.10.131

Cited by 122 publications

(78 citation statements)

References 67 publications

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“…As shown in Figure S4 , see online supplementary material , the twisted cryogel could recover to its original shape within 10 s by absorting water, which could be repeated at least 10 times. The ICP results from Table S1 (see online supplementary material ), showed that the Ag content in the cryogel was 0.015% (w/w), which was lower than that reported previously [ 56–58 ].…”

Section: Resultsmentioning

confidence: 58%

“…Moreover, the long-term antibacterial ability of CS/Gel@TA/Ag cryogel was tested for 24, 48 and 72 h. Almost no bacterial colonies could be observed on an agar plate after the cryogel treatment, indicating long-term bacterial elimination. More importantly, the Ag content in the cryogel was only 0.015% (w/w), lower than the previously reported mass [ 56–58 ], which indicated the cryogel could be safely applied in vivo .…”

Section: Discussionmentioning

confidence: 61%

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Multifunctional chitosan/gelatin@tannic acid cryogels decorated within situreduced silver nanoparticles for wound healing

Yuan

Ding

et al. 2022

Burns &Amp; Trauma

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Background Most traditional wound dressings only partially meet the needs of wound healing because of their single function. Patients usually suffer from the increasing cost of treatment and pain resulting from the frequent changing of wound dressings. Herein, we have developed a mutifunctional cryogel to promote bacterial infected wound healing based on a biocompatible polysaccharide. Methods The multifunctional cryogel is made up of a compositive scaffold of chitosan (CS), gelatin (Gel) and tannic acid (TA) and in situ formed silver nanoparticles (Ag NPs). A liver bleeding rat model was used to evaluate the dynamic hemostasis performance of the various cryogels. In order to evaluate the antibacterial properties of the prepared cryogels, gram-positive bacterium Staphylococcus aureus (S. aureus) and gram-negative bacterium Escherichia coli (E. coli) were cultured with the cryogels for 12 h. Meanwhile, S. aureus was introduced to cause bacterial infection in vivo. After treatment for 2 days, the exudates from wound sites were dipped for bacterial colony culture. Subsequently, the anti-inflammatory effect of the various cryogels was evaluated by western blotting and enzyme-linked immunosorbent assay. Finally, full-thickness skin defect models on the back of SD rats were established to assess the wound healing performances of the cryogels. Results Due to its porous structure, the multifunctional cryogel showed fast liver hemostasis. The introduced Ag NPs endowed the cryogel with an antibacterial efficiency of >99.9% against both S. aureus and E. coli. Benefited from the polyphenol groups of TA, the cryogel could inhibit nuclear factor-κB nuclear translocation and down-regulate inflammatory cytokines for an anti-inflammatory effect. Meanwhile, excessive reactive oxygen species could also be scavenged effectively. Despite the presence of Ag NPs, the cryogel did not show cytotoxicity and hemolysis. Moreover, in vivo experiments demonstrated that the biocompatible cryogel displayed effective bacterial disinfection and accelerated wound healing. Conclusions The multifunctional cryogel, with fast hemostasis, antibacterial and anti-inflammation properties and the ability to promote cell proliferation could be widely applied as a wound dressing for bacterial infected wound healing.

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Section: Resultsmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 61%

Multifunctional chitosan/gelatin@tannic acid cryogels decorated within situreduced silver nanoparticles for wound healing

Yuan

Ding

et al. 2022

Burns &Amp; Trauma

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“…The authors of [ 30 ] manufactured a high-swelling (4000%) cryogel based on gelatin and AgNPs (particle size 10–20 nm). The cryogel was active against the methicillin-resistant S. aureus (MRSA) and P. aeruginosa and promoted regeneration of the burn wound tissue in the Kunming female mice weighing 30–35 g. The wound area decreased after 2 weeks.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle-Containing Wound Dressing: Antimicrobial and Healing Effects

Yudaev

Mezhuev

Chistyakov

2022

Gels

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The dressings containing nanoparticles of metals and metal oxides are promising types of materials for wound repair. In such dressings, biocompatible and nontoxic hydrophilic polymers are used as a matrix. In the present review, we take a look at the anti-microbial effect of the nanoparticle-modified wound dressings against various microorganisms and evaluate their healing action. A detailed analysis of 31 sources published in 2021 and 2022 was performed. Furthermore, a trend for development of modern antibacterial wound-healing nanomaterials was shown as exemplified in publications starting from 2018. The review may be helpful for researchers working in the areas of biotechnology, medicine, epidemiology, material science and other fields aimed at the improvement of the quality of life.

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“…[26][27][28] Due to the presence of hydrophilic functional groups in the polymer backbone, they possess the ability to retain water. [2][3][4][5][6][7][8][9]39] On the one hand, the damp hydrogels keep the wound moist and isolates the wound from the outside environment, [40] and on the other hand, the high water absorption ensures that the hydrogel can absorb wound exudate until the dissolution equilibrium. [41,42] Thermogelling polymers consists of a large number of hydrophilic and hydrophobic fragments, and the introduction of hydrophobic fragments makes the polymer network undergoes a sol-to-gel transition when the temperature rises due to the hydrophilic and hydrophobic relationship.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Thermogelling Formulation for Burn Wound Healing

Liu

Chen

Zhu

et al. 2022

Chemistry An Asian Journal

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Burns are the most common and devastating form of wounds and are usually accompanied by abnormal inflammation, inadequate extracellular matrix production, reduced angiogenesis, and lack of growth factor stimulation, which can significantly delay wound healing and lead to complications. Pearl powder, a traditional Chinese medicine, has been used to treat wound healing. In the present study, we found that supercritical CO 2 extracted pearl peptides in the size range of " > 10kd" have great potential to promote wound healing at the cellular level. Antioxidant hydrogels were designed using selenium-containing block-functionalized PEG/PPG polymers. Pearl peptide components were combined into polymeric materials to develop new promising wound dressings. It was shown that pearl peptide hydrogels increased skin fibroblast viability, enhanced cellular resistance to oxidative stress, enhanced tissue remodeling, promoted angiogenesis, and exhibited enhanced promotion in wound healing.

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Biodegradable gelatin/silver nanoparticle composite cryogel with excellent antibacterial and antibiofilm activity and hemostasis for Pseudomonas aeruginosa-infected burn wound healing

Cited by 122 publications

References 67 publications

Multifunctional chitosan/gelatin@tannic acid cryogels decorated within situreduced silver nanoparticles for wound healing

Multifunctional chitosan/gelatin@tannic acid cryogels decorated within situreduced silver nanoparticles for wound healing

Nanoparticle-Containing Wound Dressing: Antimicrobial and Healing Effects

Antioxidant Thermogelling Formulation for Burn Wound Healing

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