Biodegradation of Chlorpyrifos Using Indigenous Pseudomonas sp. Isolated from Industrial Drain

Farhan, Muhammad; Khan, Arif Ullah; Wahid, Abdul; Ahmad, Manzoor; Ahmad, Farooq

doi:10.3923/pjn.2012.1183.1189

Cited by 27 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Karpouzas and Walker (2000) found similar results with ethoprophos degradation by P. putida , in which the presence of other C-sources had no effect on the degrading ability of the bacteria. The strains in this study preferred to utilize CP even in nutrient rich media potentially due to the constitutive expression of CP-degrading enzymes even in the presence of readily available C-sources (Anwar et al, 2009; Farhan et al, 2012). This result contrasts with previous findings of Singh et al (2004) who reported that with addition of C-sources, an Enterobacter strain stopped degrading CP and only after 3 days of incubation started utilizing CP again.…”

Section: Discussionmentioning

confidence: 99%

“…The use of microorganisms having the right metabolic pathways is one of the most viable options for the remediation of CP and TCP in soil and water (Li et al, 2010; Thengodkar and Sivakami, 2010; Singh et al, 2011). Previously, CP was reported to be resistant to degradation (Racke et al, 1990; Mallick et al, 1999), but later studies identified bacteria from the genera Enterobacter (Singh et al, 2004), Pseudomonas (Lakshmi et al, 2009; Farhan et al, 2012; Chawla et al, 2013), Bacillus (Liu et al, 2012; El-Helow et al, 2013), and Klebsiella (Ghanem et al, 2007) that were able to degrade CP efficiently. In other recent studies, it was further determined that some of CP-degrading bacterial strains from the genera Bacillus (Anwar et al, 2009), Alcaligenes (Yang et al, 2005), Paracoccus (Xu et al, 2008), Gordonia (Abraham et al, 2013), Sphingobacterium (Abraham and Silambarasan, 2013), and Mesorhizobium (Jabeen et al, 2015) could utilize CP as a sole source of carbon (C) and also degrade TCP.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils

et al. 2017

View full text Add to dashboard Cite

Chlorpyrifos (CP) is one of the most widely used organophosphate pesticides in agriculture worldwide, but its extensive use has led to the contamination of various soil and water systems. Microbial bioremediation is considered to be one of the most viable options for the removal of CP from the environment; however, little is known about the soil bacterial diversity that degrade CP. Sequential soil and liquid culture enrichments enabled the isolation of bacterial CP degraders with sequence homologies to Xanthomonas sp., Pseudomonas sp., and Rhizobium sp. The efficacy of the three isolated strains: Xanthomonas sp. 4R3-M1, Pseudomonas sp. 4H1-M3, and Rhizobium sp. 4H1-M1 was further investigated for biodegradation of CP and its primary metabolic product, 3,5,6-trichloro-2-pyridinol (TCP). The results indicate that all three bacterial strains almost completely metabolized CP (10 mg/L) and TCP, occurring as a metabolic degradation product, in mineral salt media as a sole source of carbon and nitrogen. The isolated bacterial strains Xanthomonas sp. 4R3-M1 and Pseudomonas sp. 4H1-M3 could also degrade TCP (10 mg/L) as a sole carbon and nitrogen source, when provided externally. Thus, these bacterial strains may be effective in practical application of bioremediation of both CP and TCP.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils

et al. 2017

View full text Add to dashboard Cite

show abstract

“…During previous study, bacterial strains able to degrade chlorpyrifos were isolated from wastewater collected from drains receiving wastewater from different industries (Farhan et al, 2012). The same strain was used for soil experimentation in this study.…”

Section: Bio-augmentation Experimentmentioning

confidence: 99%

Restoration of Pesticide Contaminated Agricultural Soil Through Bio-Augmentation and Its Kinetics

Farhan¹

2017

PAKJAS

Self Cite

View full text Add to dashboard Cite

Excessive use of pesticides is evident in third world countries and Pakistan is not the exception to this. Most of these pesticides are persistent and they remain present in ecosystem as active ingredient for longer duration. Their presence cause serious damage to micro flora and fauna, thus disturbing the functioning of ecosystems. After absorbance, they enter into food chain and tend to bio-accumulate. In the present situation, bio-augmentation and bio-stimulation are considered most safe techniques to clean pesticide contaminated soils. These techniques are natural, cost effective, do not produce toxic by products and easy to handle. The present study was designed to degrade chlorpyrifos (CP) in laboratory soil using Pseudomonas sp., which was isolated from industrial drain. This strain showed promising results in both liquid medium and soil. This strain degraded up to 60mg of CP per one Kg of soil. Among different organic amendments, farm yard manure was most effective and showed highly significant results. Kinetic analysis and its data also provide strong evidence of success. Kinetic parameters were as follow; R 2 = 0.9917, K s = 159.0271, V max = 9.64 and V max /K s = 0.0606. This research is successful as it provided indigenous solution to a environmental problem.

show abstract

“…Human exposure to CPF may cause chronic effects such as impaired memory and concentration, headache, confusion, nausea, weakness, etc. [7][8][9][10]. Use of chlorpyrifos causes greater health risk when it exceeds acute exposure levels [11].…”

Section: Introductionmentioning

confidence: 99%

Comparing geometric parameters of a hydrodynamic cavitation process treating pesticide effluent

Randhavane

2018

Environmental Engineering Research

View full text Add to dashboard Cite

Paper focuses on comparison between two different orifice plate configurations (plate number 1 and plate number 2) used as cavitating device in the hydrodynamic cavitation reactor for improving pollutant removal efficiencies. Effect of four different parameters such as hydraulic characteristics (in terms of range of flow rates, orifice velocities, cavitation number at different inlet pressures); cavitation number (in range of 5.76-0.35 for plate number 1 and 1.20-0.35 for plate number 2); inlet pressure (2-8 bars) and reaction time (0 to 60 min) in terms of chemical oxygen demand (COD) removal and chlorpyrifos degradation has been studied and compared. Optimum inlet pressure of 5 bars exists for degradation of pollutants for both the plates. It is found that geometry of orifice plate plays important role in removal efficiencies of pollutant. Results obtained confirmed that orifice plate 1 with configuration of 1.5 mm 17 holes; cavitational number of 1.54 performed better with around 60% COD and 98% chlorpyrifos removal as compared to orifice plate 2 having configuration of 2 mm single hole; cavitational number of 0.53 with 40% COD and 96% chlorpyrifos in 2 h duration time.

show abstract

Biodegradation of Chlorpyrifos Using Indigenous Pseudomonas sp. Isolated from Industrial Drain

Cited by 27 publications

References 22 publications

Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils

Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils

Restoration of Pesticide Contaminated Agricultural Soil Through Bio-Augmentation and Its Kinetics

Comparing geometric parameters of a hydrodynamic cavitation process treating pesticide effluent

Contact Info

Product

Resources

About