Biodegradation of feather keratin with a PEGylated protease of Chryseobacterium gleum

Chaudhari, Priti N.; Chincholkar, S. B.; Chaudhari, Bhushan L.

doi:10.1016/j.procbio.2013.09.011

Cited by 11 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Huge amount of keratin byproducts are wasted without ample utility (Korniłłowicz-Kowalska and Bohacz 2011 ). Several attempts have been made for the mechanical (Korol 2012 ) as well as microbial utilization of keratin wastes (Chaudhari et al 2013 ; Fang et al 2013 ; Jeong et al 2010 ). Keratin like other natural biopolymers viz., collagen and chitosan can be used for making biomaterials in tissue repair and regeneration (Alsarra 2009 ; Natarajan et al 2012 ; Ramshaw et al 2009 ; Ramadass et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of keratin from bovine hoof: A potential material for biomedical applications

2014

View full text Add to dashboard Cite

Keratin from the hoof is a less explored source for making valuable products. In this paper we present the extraction of pure keratin from bovine hooves and characterized them to better address the possible exploitation of this bio-resource as an alternative material for tissue engineering applications. The keratin protein from the pulverized hooves was extracted by reduction, which was observed to be pure, and two polypeptide chains of molecular weight in the range of 45–50 and 55–60 KDa were determined using SDS-PAGE assay. FTIR analysis complementing circular dichroism (CD) data, established that hoof keratin predominantly adopted α-helical conformation with admixture of β-sheet. The keratin was shown to have appreciably high denaturation temperature (215°C) as indicated by differential scanning calorimetric (DSC) analysis. Thermogravimetric analysis (TGA) also showed the retention of 50% of the original weight of the sample even at a temperature of 346°C. The keratin from the hoof had been observed to be biocompatible when analyzed with MTT assay using fibroblast cells, showing more than 90% cell viability. Hence, hoof keratin would be useful for high value biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of keratin from bovine hoof: A potential material for biomedical applications

2014

View full text Add to dashboard Cite

show abstract

“…[4] Annually, a large amount of keratin waste is generated by poultry slaughterhouses [5] therefore several efforts have been made for the chemical and also microbial utilization of poultry keratin wastes. [6][7][8] The main challenge in this work is that the selected technique used for dissolving the keratin should also be able to breakdown resistant bands with minimal damage and degradation of the primary polypeptide chains. Therefore, a combination of chemical methods and enzymatic hydrolysis can provide a remarkable opportunity to produce the hydrolyzed products of keratin with value-added properties being eco-friendly and cost effective.…”

Section: Introductionmentioning

confidence: 99%

“…Protein keratin can be applied for making bio-materials in tissue regeneration, repair, drug delivery, and biosensors like other natural biopolymers (chitosan and collagen). [5,6,16,17] Some reports have demonstrated that the value of keratins may be increased when used in biotechnology and biomedical applications. [18] The extracellular matrix keratin polypeptide contains cell adhesion sequences viz., LDV (Leu-Asp-Val), and also RGD (Arg-Gly-Asp) which are also recognized in the fibronectin.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ultrasound‐Assisted Alkaline Extraction on Antioxidant Activity and Functional Characteristics of Chicken Feather Keratin Peptides

Alahyaribeik

Ullah

2020

ChemistrySelect

View full text Add to dashboard Cite

In this research, we focus on enzymatic hydrolysis of chicken feathers, after chemical or chemical and ultrasound pretreatment. Our result illustrated that ultrasound pretreatment preserved the main structure and amino acid composition of extracted keratin as confirmed by the Fourier-transform infrared (FTIR) spectroscopy and gel-electrophoresis analysis. In addition, investigation showed that ultrasound-assisted chemical treatment was more effective on enzymatic hydrolysis and bioactivity as compared to only the chemical treatment. Thermo gravimetric analysis (TGA) characterization of hydro-lyzed products, using Alcalase enzyme at different times, showed that decomposition temperature was decreased during enzymatic hydrolysis and the best antioxidant activity was achieved after 30 min of hydrolysis with the value of 90.43 � 4.03 % (5 mg/ml concentration). Also, it was found that hydrolyzed product indicated no cytotoxicity to SK-N-MC cells when the concentration is less than 0.312 mg/ml. In this paper, the results demonstrated that the ultrasonic-assisted enzymolysis processing seems to be effective for producing keratin antioxidant hydrolysate.

show abstract

“…In the form of solid biomass keratin is less prone to enzymatic hydrolysis due to high cross-linking by disulfide bonds, hydrophobic interactions and hydrogen bonding ( Teresa and Justyna 2011). In recent years, several attempts were made for the extraction of keratin using chemical, mechanical and enzymatic methods ( Jeong et al 2010;Korol 2012;Chaudhari et al 2013;Fang et al 2013). One of the potential application of purified keratin is to generate biomaterials in tissue repair and regeneration (Alsarra 2009;Ramshaw et al 2009;Natarajan et al 2012;Ramadass et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives

Sharma

Gupta

2016

Braz. arch. biol. technol.

164

102

View full text Add to dashboard Cite

show abstract

Biodegradation of feather keratin with a PEGylated protease of Chryseobacterium gleum

Cited by 11 publications

References 34 publications

Extraction and characterization of keratin from bovine hoof: A potential material for biomedical applications

Extraction and characterization of keratin from bovine hoof: A potential material for biomedical applications

Effects of Ultrasound‐Assisted Alkaline Extraction on Antioxidant Activity and Functional Characteristics of Chicken Feather Keratin Peptides

Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives

Contact Info

Product

Resources

About