Biodegradation of fluorene and bioremediation study by Sphingobacterium sp. KM-02 isolated from PAHs-contaminated soil

Abstract

The fluorene-degrading strain Sphingobacterium sp. KM-02 was isolated from PAHs-contaminated soil near a mineimpacted area by selective enrichment techniques. Fluorene added to the Sphingobacterium sp. KM-02 culture as sole carbon source was 78.4% removed within 120 h. A fluorene degradation pathway is tentatively proposed based on identification of the metabolic intermediates 9-fluorenone, 4-hydroxy-9-fluorenone, and 8-hydroxy-3,4-benzocoumarin.Further the ability of Sphingobacterium sp. KM-02 to bioremediate 100 mg/kg fluorene in soil matrix was examined by composting under laboratory conditions. Treatment of microcosm soil with the strain KM-02 for 20 days resulted in a 65.6% reduction in total amounts. These results demonstrate that Sphingobacterium sp. KM-02 could potentially be used in the bioremediation of fluorene from contaminated soil.

Heavy Metal Effects on the Biodegradation of Fluorene by Sphingobacterium sp. KM-02 in liquid medium

“…토양에 노출된 대부분의 중금속들은 토양 입자에 흡착되어 존재하며, 이온 형태로 산화 및 환원되어 보다 자유롭게 움직일 수 있어서, Al, Co, Cd, Ni, Cu, Zn, Pb, Hg 등 다양한 종류의 중금속들이 생화학적 산소요구 량(BOD, Biological Oxygen Demand) 혹은 유기오염물 질 분해작용을 저해하는 등 다양한 부작용을 야기한다는 많은 결과들을 찾을 수 있다 (Amor et al, 2001;Baath, 1989;Nies, 1999;Mittal and Ratra, 2000;Sokhn et al, 2001). 그러나, 현재까지 Fluorene을 포함한 PAHs (Nam et al, 2011). 46.4 ± 6.4 17.4 ± 2.6 15.4 ± 2.5 Cu 26.9 ± 4.6 14.6 ± 3.6 11.5 ± 1.9 10.9 ± 0.9 Cd 11.3 ± 1.8 22.1 ± 2.0 9.0 ± 0.…”

“…또한, 미국 EPA에서 지정한 PAHs 우선규제대상물질 중 Fluorene(C 13 H 10 )과 Phenanthrene (C 14 H 10 )이 오염지역에서 가장 빈번하게 나타나고 있어 저 분자량 PAHs의 처리가 더욱 중요하다고 보고한 바 있다 (Amor et al, 2001;Baath, 1989). 기존 연구들에서 Fluorene은 다양한 미생물에 의해 분해되는 것으로 보고 되고 있으며, 해당 균주는 Pseudomonas, Arthrobacter, Staphylococcus, Mycobacterium, Sphingomonas, Brevibacterium, Acinetobacter, Sphingobacterium 등이 있다 (Casellas et al, 1997;Grifoll et al, 1994;Monna et al, 1993;Nam et al, 2011;Wattiau et al, 2001;Pagnout et al, 2007;Schuler et al, 2008;Thangaraj et al, 2008).…”