Biodegradation of mixture containing monohydroxybenzoate isomers by Acinetobacter calcoaceticus

Prathibha, K.; Suresh, S.

doi:10.1007/s11274-007-9545-x

Cited by 8 publications

(4 citation statements)

References 47 publications

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“…Microbial degradation of phenolic compounds ( Yu, 2001 ) is cost-effective and environmentally friendly ( De et al, 2006 ). Strain 11 of Acinetobacter calcoaceticus is able to utilize a phenolic mixture of PHBA, 2-hydroxybenzoic acid, and 3-hydroxybenzoic acid in medium ( Prathibha and Sumathi, 2008 ). A. calcoaceticus DSM586 can grow on PHBA as sole carbon source and also has a capacity of degrading FA in medium ( Delneri et al, 1995 ).…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

et al. 2018

Front. Microbiol.

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Ferulic acid (FA) and p-hydroxybenzoic acid (PHBA) are main phenolic compounds accumulated in rhizosphere of continuously cropped cucumber, causing stress in plants. Microbial degradation of a mixture of FA and PHBA is not well understood in soil. We isolated a strain CSY-P13 of Acinetobacter calcoaceticus, inoculated it into soil to protect cucumber from FA and PHBA stress, and explored a mechanism underlying the protection. CSY-P13 effectively degraded a mixture of FA and PHBA in culture solution under conditions of 39.37°C, pH 6.97, and 21.59 g L-1 potassium dihydrogen phosphate, giving rise to 4-vinyl guaiacol, vanillin, vanillic acid, and protocatechuic acid. During FA and PHBA degradation, activities of superoxide dismutase (SOD), catalase, ascorbate peroxidase, and dehydroascorbate reductase in CSY-P13 were induced. Inoculated into cucumber-planted soil containing 220 μg g-1 mixture of FA and PHBA, CSY-P13 degraded FA and PHBA in soil, increased plant height, and decreased malonaldehyde, superoxide radical, and hydrogen peroxide levels in leaves. CSY-P13 also enhanced SOD, guaiacol peroxidase, catalase, glutathione peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase activities; increased ascorbate and glutathione contents; and elevated transcript levels of copper/zinc SOD, manganese SOD, and catalase in leaves under FA and PHBA. Moreover, CSY-P13 increased phosphatase, catalase, urease, and sucrase activities and changed bacterial richness, diversity, and community composition by high throughput sequencing in cucumber-planted soil supplemented with the mixture of FA and PHBA. So CSY-P13 degrades the mixture of FA and PHBA in soil and mitigates stress from the two phenolic compounds in cucumber by activating antioxidant enzymes, changing soil bacterial community, and inducing soil enzymes.

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Section: Introductionmentioning

confidence: 99%

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

et al. 2018

Front. Microbiol.

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“…Acinetobacter are known to degrade a broad variety of hydrocarbons, like n-alkanes [43], aromatic compounds [44] and diesel oil [45]. Due to their profound adaptability and metabolic versatility, Acinetobacter species can be widely found in soil [46], seawater [47], waste water [48], sewage [49], oil [50] and clinical specimens [51].…”

Section: Identification By 16s Rrna Sequence Analysismentioning

confidence: 99%

Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability

Onur

Yılmaz

İçgen

2015

J Surfact & Detergents

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Degradation of poorly water soluble hydrocarbons, like n‐alkanes and polycyclic aromatic hydrocarbons are challenged by some bacteria through emulsification of hydrocarbons by producing biosurfactants. In diesel oil bioremediation, diesel oil degrading and surfactant producing bacteria are used to eliminate these pollutants from contaminated waters. Therefore, identifying and characterizing bacteria capable of producing surfactant and degrading diesel oil are pivotal. In this study, bacteria isolated from hydrocarbon contaminated river water were screened for their potential to degrade diesel oil. Primary selection was carried out by using conventional enrichment culture technique, emulsification index measurement, gravimetric and gas chromatographic analyses of diesel oil degradation. A bacterium with 60 % emulsification index and 92 % diesel oil degradation ability in 14 days was identified as Acinetobacter haemolyticus Zn01 by 16S rRNA sequencing. A. haemolyticus Zn01 was shown to harbor both catabolic genes alkB and C23O effective in diesel oil degradation. The biosurfactant of the bacterium was also characterized in terms of surface tension, zeta potential, Fourier transform infrared spectroscopy and scanning electron microscopy. Being able to emulsify and degrade diesel oil, A. haemolyticus Zn01 seems to have high potential for the elimination of diesel oil from polluted waters.

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“…The method of Prathibha and Suresh was used with some modifications [12]. Bacterial cells grown on phthalate esters were harvested at mid exponential phase (~17 h) by centrifugation at 4°C.…”

Section: F Preparation Of Cell-free Extractsmentioning

confidence: 99%

Biodegradation of Dimethyl Phthalate, Diethyl Phthalate, Dibutyl Phthalate and Their Mixture by VariovoraxSp

Prasad¹,

Suresh²

2012

IJESD

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Biodegradation of dimethyl phthalate (DMP), diethyl phthalate (DEP) and dibutyl phthalate (DBP) and their mixture by a bacterial strain is reported. The strain was isolated by enrichment technique from garbage dumped soil nearby Indian Institute of Technology Bombay. The isolate had the ability to utilize mixture of DMP, DEP and DBP in the growth medium as well as the individual phthalate esters as the sole source of carbon and energy. The bacterial isolate was identified as Variovorax sp. based on 16S r-DNA sequence analysis. Variovorax sp. degraded >99% of 300 mg/l of DMP, DEP, DBP or a mixture containing 100 mg/l of each within 30 h. Metabolites or end products were not detected by HPLC analyses. Biodegradation of phthalate esters followed first-order kinetics. The rates of biodegradation of individual esters followed the order: DEP>DBP>DMP while in the mixture, rates followed the order: DEP>DMP>DBP. Crude cell free extracts prepared from bacterial isolates contained cocktail of inducible enzymes responsible for the degradation of DMP or DEP.

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Biodegradation of mixture containing monohydroxybenzoate isomers by Acinetobacter calcoaceticus

Cited by 8 publications

References 47 publications

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

Acinetobacter calcoaceticus CSY-P13 Mitigates Stress of Ferulic and p-Hydroxybenzoic Acids in Cucumber by Affecting Antioxidant Enzyme Activity and Soil Bacterial Community

Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability

Biodegradation of Dimethyl Phthalate, Diethyl Phthalate, Dibutyl Phthalate and Their Mixture by VariovoraxSp

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