Biodegradation of Phenol by Antarctic Strains of Aspergillus fumigatus

Abstract

Taxonomic identifi cation of three newly isolated Antarctic fungal strains by their 18S rDNA sequences revealed their affi liation with Aspergillus fumigatus. Phenol (0.5 g/l) as the sole carbon source was completely degraded by all strains within less than two weeks. Intracellular activities of three key enzymes involved in the phenol catabolism were determined. Activities of phenol hydroxylase (EC 1.14.13.7), hydroquinone hydroxylase (EC 1.14.13.x), and catechol 1,2-dioxygenase (EC 1.13.11.1) varied signifi cantly between strains. The rates of phenol degradation in the three strains correlated best with the activity of catechol 1,2-dioxygenase. Six pairs of oligonucleotide primers were designed on the basis of the Aspergillus fumigatus Af293 genome sequence (NCBI Acc. No. XM_743491.1) and used to amplify phenol hydroxylase-related gene sequences. DNA sequences of about 1200 bp were amplifi ed from all three strains and found to have a high degree of sequence identity with the corresponding gene of Aspergillus fumigatus Af293

Molecular Dynamics Simulation of Phenol Aqueous Solution under the Impact of an External Electrostatic Field

“…Thus, it is often studied as a typical pollutant. There are a lot of methods for phenol removal from wastewater, such as electrodeposition, oxidation, adsorption, and biological treatment. − …”

Fenton-Mediated Chlorophenol Degradation by Iron-Reducing Compounds Isolated from Endophytic Fungi in Atacama Puna Plateau Lecanicillium ATA01

“…The ferri-IRCs can mediate the hydroxylation of chlorophenols, and catechol was obtained as an intermediate in the degradation pathways of various aromatic compounds [42]. Similar evidence of hydroxylated metabolites derived from the 2,4-dichlorophenol degradation by Gloeophyllum striatum has been provided by Schlosser et al [43].…”