Biodegradation of Plant Pectin and Hemicelluloses with Three Novel Bacillus pumilus Strains and Their Combined Application for Quality Jute Fibre Production

Das, Suparna; Majumdar, B.; Saha, A. R.

doi:10.1007/s40003-015-0188-0

Cited by 20 publications

(44 citation statements)

References 32 publications

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“…Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms [10][11][12][13] . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting [14][15][16][17][18] along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 .…”

mentioning

confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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Jute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute retting water samples in Bangladesh. Higher pectinolytic bacterial isolates were predominant in the later stage of jute retting. Out of these, 168 isolates have been screened by both semi-quantitative and quantitative pectinase, xylanase and cellulase enzyme assay. Among them, 144 isolates have been selected on the basis of extra cellular enzyme activity of these three enzymes. 16 s ribosomal gene sequencing analysis identified 2 phyla-Firmicutis (80.55%) and Proteobacteria (19.45%). To check the synergistic and antagonistic effect 10 selected isolates were tested in 167 different combinations. Three best combinations were identified that lowered retting period from 18-21 days to 10 days producing high quality fibre in both laboratory and field trial. This improved retting technology can be adopted in industrial scale for the production of quality jute fibre in a controlled condition in reduced water quantity without polluting the environment.Jute, second most important natural fibre after cotton, is cultivated in East Asia and some parts of Latin America 1-3 . Jute is a bast or phloem fibre in the bark of stems, cemented together by pectin and gummy substances 4 . Commercial extraction of jute fibre is water based microbiological retting where jute bundles are submerged into slow running river water and subjected to decomposition of pectin, hemicelluloses, and other mucilaginous substances 2,5 . In this process, pectin is depolymerized by pectinases, primarily comprising four enzymes: Polygalacturonase (PG), Pectin Lyase (PNL), Pectate lyase and Pectin esterase. However, PG 6 , and PNL 7 are primary retting enzymes. In addition, xylanase makes jute fibre softer by selective removal of non-fibrous hemicelluloses without affecting strength of cellulosic fibre. Pectinolytic microorganisms having xylanase activity devoid of cellulase activity is an additional beneficial aspect to improve fibre quality 8 . The quality of fibre is largely determined by the efficiency of retting process 1,2,9,10 and various factors are responsible for proper retting as well as improved fibre quality. Most promising water based microbiological retting process mostly involves bacteria along with various fungi, protozoa, algae and diatoms 10-13 . Main aerobic retting bacteria belong to genus Bacillus viz., B. subtilis, B. polymyxa, B. mesentericus, B.pumilus, B. cereus, B. megaterium and B. macerans, initiate retting 14-18 along with large numbers of gram-negative genera such as Erwinia and Pseudomonas 9,19 . At the later stage of retting some anaerobic bacteria from genus Clostridium -Clostridium acetobutylicum, Clostridium stercorarium, Clostridium tertium come to carry on the retting process 20 . As microorganisms are the ma...

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confidence: 99%

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Hasan

Aktar

Kabir

et al. 2020

Sci Rep

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“…The polygalacturonase and pectin lyase production in yeast extract pectin agar media by microbial consortium 3 were higher by 2.2 and 3.3 times over microbial consortium 1 and 2.8 and 3.94 times over microbial consortium 2. Yeast extract pectin media at alkaline pH (8-10) recorded the maximum polygalacturonase and pectin lyase enzyme production by pectinolytic microbial consortia used for jute retting materials helps in the loosening of the bondage with cellulosic fibre and easy removal of cementing portion, softening of bark tissues to obtain better quality fibre (Das et al, 2015). Higher fibre recovery and quality jute fibre production with reduction in retting duration in improved retting by using microbial consortium compared to conventional retting was reported by .…”

Section: Issn: 2319-7706 Volume 6 Number 11 (2017) Pp 925-931mentioning

confidence: 89%

“…Microbial consortium 1 (MC 1) consists of 4 pectinolytic bacteria of Bacillus sp.L6, Bacillus pumilus EK 17, B. pumilus Geo-03-422 and B. pumilus IK-MB12-518 F (Das et al, 2012), microbial consortium 2 (MC2) consists of three different strains of Bacillus pumilus and one strain of Bacillus subtilis (Srivastava et al, 2012) and microbial consortium 3 (MC 3) consists of Bacillus pumilus IMAU80221, B. pumilus GVC11 and B. pumilus SYBC-W (Majumdar et al, 2009;Das et al, 2015). The microbes of MC1 & MC2 have cellulolytic activities whereas; the microbes of MC3 do not have any cellulolytic activities.…”

Section: Microbial Consortiummentioning

confidence: 99%

“…secreted by the retting microbes. Uses of pectinolytic bacterial consortium for quick retting of jute have been reported by several researchers (Banik et al, 2007;Majumdar et al, 2009;Das et al, 2012;Das et al, 2015). The microbes of microbial consortium in useful association release pectinolytic enzymes (polygalacturonase, pectin lyase) and xylanase, which in turn degrade the pectin and xylan present as cementing materials.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting

Nath¹,

Majumdar²,

Das³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Although jute retting is better in slow moving soft water, only 10 % of jute growers of India have access to this and the remaining 90% ret their harvest in stagnant water bodies like lakes, ditches and ponds. Use of microbial inoculants to hasten the jute retting in stagnant water retting has been reported by several workers (Banik et al, 2007;Das et al, 2012;Das et al, 2015;Das et al, 2018). The major drawback in using the carrier based microbial inoculants either as biofertilizer/ biopesticides or bioformulation is their short shelf life, as the microbial cells cannot withstand environmental stress for prolonged period.…”

Section: Introductionmentioning

confidence: 99%

Use of bacterial endospore with longer shelf-life in improved retting of jute

Chattopadhyay¹,

Majumdar²,

Mazumdar³

et al. 2019

JEB

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Biodegradation of Plant Pectin and Hemicelluloses with Three Novel Bacillus pumilus Strains and Their Combined Application for Quality Jute Fibre Production

Cited by 20 publications

References 32 publications

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Pectinolytic Bacterial Consortia Reduce Jute Retting Period and Improve Fibre Quality

Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting

Use of bacterial endospore with longer shelf-life in improved retting of jute

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