Biodiesel production using a mixture of immobilizedRhizopus oryzae andCandida rugosa lipases

Current status and new developments of biodiesel production using fungal lipases

“…It has also been reported that the use of a mixture of 1,3-specific immobilized ROL and non-specific C. rugosa lipases could increase the operational stability of the lipases [76,135]. Biodiesel conversion above 98% was reached in 4 h using a mixture of ROL and C. rugosa lipases in a ratio of 3:1, and ethanol addition in steps.…”

“…Initially, the studies for enzymatic biodiesel preparation were carried out using free fatty acids (mainly C18:1) and refined oils, including soybean (Glycine max L.) [31,40,53,76], rapeseed (Brassica napus L.) [34,37], sunflower (Helianthus annuus) [31,37,77], olive (Olea europaea L.) [31], cotton (Gossypium hirsutum L.) [33,78], palm (Elaeis guineensis) [3,79] and other source of oils, which are suitable for the evaluation of enzyme performance [14,80]. Corn oil (Zea mays), safflower oil (Carthamus tinctorius L.) and linseed oil (C. tinctorius) were used as feedstock to produce biodiesel by Tan et al [8], and conversions higher than 90% were obtained using lipase from Candida sp.…”

Estimating and improving cold filter plugging points by blending biodiesels with different fatty acid contents

“…Biodiesel is generally manufactured by acid-, alkali-, and enzyme-catalyzed, or supercritical non-catalyzed, trans-*Corresponding author Tel: +82-62-530-1841 Fax: +82-62-530-1910 e-mail: dhpark@chonnam.ac.kr † The first two authors equally contributed to this work. esterification processes [2,4,5,7,8].…”

Synthesis of Fatty Acid Methyl Esters Using Mixed Enzyme in a Packed Bed Reactor

“…95 in a batch system 17 . Lee et al determined that the mixed enzyme of R. oryzae lipase and C. rugosa lipase gave a higher yield of biodiesel than a single lipase 19 . There have also been some limited research regarding the synthesis of biodiesel using a mixed enzyme in a continuous reactor, but the reported yield was significantly lower than our present results 20 .…”