Bioelectrochemistry of cell surfaces

Dołowy, Krzysztof

doi:10.1016/0079-6816(84)90013-3

Cited by 45 publications

(20 citation statements)

References 241 publications

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“…Cell membrane charge has been found to increase during tumorigenesis and decrease during necrosis (Dołowy, 1984). Correspondingly, investigations of factors that influence membrane electric charge during cancer transformation have been performed.…”

Section: Electric Properties Of Cell Membranesmentioning

confidence: 99%

Characterization of the Cell Membrane During Cancer Transformation

Szachowicz-Petelska¹,

Dobrzyńska²,

Sulkowski³

et al. 2012

Colorectal Cancer Biology - From Genes to Tumor

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Section: Electric Properties Of Cell Membranesmentioning

confidence: 99%

Characterization of the Cell Membrane During Cancer Transformation

Szachowicz-Petelska¹,

Dobrzyńska²,

Sulkowski³

et al. 2012

Colorectal Cancer Biology - From Genes to Tumor

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“…Ultrasound has also shown a similar correlation of FCD in the extracellular matrix with physiological and pathological changes in tissue [33]. Dolowy [34] has demonstrated that cell membrane charge increases during tumorogenesis and decreases during necrosis.…”

Section: Discussionmentioning

confidence: 92%

Imaging the electro-kinetic response of biological tissues with phase-resolved optical coherence tomography

Demidov¹,

Toronov²,

Xu³

et al. 2014

Photonics &Amp; Lasers in Medicine

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In this study, the electro-kinetic phenomena (EKP) induced in biological tissue by external electric field, while not directly visible in optical coherence tomography (OCT) images, were detected by analyzing their textural speckle features. During application of a low-frequency electric field to the tissue, speckle patterns changed their brightness and shape depending on the local tissue EKP. Since intensities of OCT image speckle patterns were analyzed and discussed in our previous publications, this work is mainly focused on OCT signal phase analysis. The algorithm for extracting local spatial phase variations from unwrapped phases is introduced. The detection of electrically induced optical changes manifest in OCT phase images shows promise for monitoring the fixed charge density changes within tissues through their electro-kinetic responses. This approach may help in the identification and characterization of morphology and function of healthy and pathologic tissues.

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“…There is an increase in the positive surface charge of cancer cells at low pH until a plateau is reached. At high pH, the negative surface charge of the cells also increases, reaching a plateau [37]. Therefore, we propose that *rINF-a-2b-Pt-GMs-15 because of their positive charge would adhere to the negatively charged SKOV3 cells and release *rINF-a-2b by polymerization and diffusion so that a high and effective concentration is reached to induce apoptosis.…”

Section: Discussionmentioning

confidence: 96%

Sustained-release protamine sulphate-impregnated microspheres may reduce the frequent administration of recombinant interferon α-2b in ovarian cancer

Gulia

Rai²,

Jain³

et al. 2014

Anti-Cancer Drugs

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Parenteral administration of recombinant interferon-α-2b (rINF-α-2b) at a dose of 50×10 IU once a week for 8 weeks is recommended for ovarian cancer. However, short half-life, small therapeutic index and proteolytic degradation cause fluctuations in plasma level and pose barriers in the development of a clinically viable dosage form. Therefore, in the present investigation, fluorescein isothiocynate-tagged rINF-α-2b was loaded into stearic acid (*rINF-α-2b-SMs), pectin (*rINF-α-2b-PMs) and gelatin (*rINF-α-2b-GMs) microspheres. Parameters such as particle size, ζ potential, encapsulation efficiency and in-vitro release were studied to follow the optimization process. The formulation, *rINF-α-2b-GMs of particle size 8.3±2.1 μm with an encapsulation efficiency of 76.0±7.4%, offered 97.4% of *rINF-α-2b release at 288 h. Thus, negatively charged extended-release formulation *rINF-α-2b-GMs was then tethered with a gradient concentration (5-20 mg/ml) of a cationic arginine-rich protein stabilizer, protamine sulphate (Pt). The nanoformulation, *rINF-α-2b-Pt-GMs-15 superimposed with 15 mg/ml of Pt, released 95.0% of *rINF-α-2b at 336 h and was designated as the optimized formulation. The optimized formulation also conserved the primary and secondary structure of *rINF-α-2b as analysed by gel electrophoresis and circular dichroism. Moreover, in-vitro cytotoxicity analysis of SKOV3 cells of the optimized nanoformulation reported significantly (one-way analysis of variance test, P<0.05) lower IC50 (414.3 IU/ml) compared with *rINF-α-2b-GMs (514.3 IU/ml) and pure rINF-α-2b (628.6 IU/ml) at 72 h by offering a prolonged cytotoxic effect. Therefore, *rINF-α-2b-Pt-GMs-15, a promising nanomedicine, warrants further in-depth in-vivo study to scale up the technology for clinical translation.

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Bioelectrochemistry of cell surfaces

Cited by 45 publications

References 241 publications

Characterization of the Cell Membrane During Cancer Transformation

Characterization of the Cell Membrane During Cancer Transformation

Imaging the electro-kinetic response of biological tissues with phase-resolved optical coherence tomography

Sustained-release protamine sulphate-impregnated microspheres may reduce the frequent administration of recombinant interferon α-2b in ovarian cancer

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