Biofabrication and in vitro study of hydroxyapatite/mPEG–PCL–mPEG scaffolds for bone tissue engineering using air pressure-aided deposition technology

Jiang, Cho‐Pei; Chen, Yo-Yu; Hsieh, Min-Fa

doi:10.1016/j.msec.2012.10.018

Cited by 22 publications

(18 citation statements)

References 26 publications

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“…The melting point determined by DSC was at 53.6°C. Compared to our previous paper where the melting point of PEG-PCL having number-average Mw of 17,217 Da was 57.2°C, the mPEG-PCL used in the current study has lower Mw and yet lower melting point [32]. …”

Section: Resultsmentioning

confidence: 67%

The mPEG-PCL Copolymer for Selective Fermentation of Staphylococcus lugdunensis Against Candida parapsilosis in the Human Microbiome

Kao

Wang

Marito

et al. 2016

J Microb Biochem Technol

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Many human skin diseases, such as seborrheic dermatitis, potentially occur due to the over-growth of fungi. It remains a challenge to develop fungicides with a lower risk of generating resistant fungi and non-specifically killing commensal microbes. Our probiotic approaches using a selective fermentation initiator of skin commensal bacteria, fermentation metabolites or their derivatives provide novel therapeutics to rein in the over-growth of fungi. Staphylococcus lugdunensis (S. lugdunensis) bacteria and Candida parapsilosis (C. parapsilosis) fungi coexist in the scalp microbiome. S. lugdunensis interfered with the growth of C. parapsilosis via fermentation. A methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (mPEG-PCL) copolymer functioned as a selective fermentation initiator of S. lugdunensis, selectively triggering the S. lugdunensis fermentation to produce acetic and isovaleric acids. The acetic acid and its pro-drug diethyleneglycol diacetate (Ac-DEG-Ac) effectively suppressed the growth of C. parapsilosis in vitro and impeded the fungal expansion in the human dandruff. We demonstrate for the first time that S. lugdunensis is a skin probiotic bacterium that can exploit mPEG-PCL to yield fungicidal short-chain fatty acids (SCFAs). The concept of bacterial fermentation as a part of skin immunity to re-balance the dysbiotic microbiome warrants a novel avenue for studying the probiotic function of the skin microbiome in promoting health.

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Section: Resultsmentioning

confidence: 67%

The mPEG-PCL Copolymer for Selective Fermentation of Staphylococcus lugdunensis Against Candida parapsilosis in the Human Microbiome

Kao

Wang

Marito

et al. 2016

J Microb Biochem Technol

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“…The absorption peak for phosphate (PO 4 ) group of HA at around 1029 cm −1 (see Ref. ) observed in the spectrum of 75PCLDA/HA hydrogel.…”

Section: Resultsmentioning

confidence: 94%

“…Poly(ethyleneglycol) (PEG) has been an important type of hydrophilic polymers for biomedical applications because they have critical properties, such as good biocompatibility, hydrophilicity, lack of toxicity, and absence of antigenicity and immunogenicity . Hydroxyapatite (HA) is a ceramic material with a composition and structure similar to natural bone mineral and has been demonstrated to have good biocompatibility, and even osteoconductivity and osteoinductivity . Some studies reported that inclusion of HA into PCL scaffold improved osteoconductivity and produced mineralized nodules .…”

Section: Introductionmentioning

confidence: 99%

“…Some studies reported that inclusion of HA into PCL scaffold improved osteoconductivity and produced mineralized nodules . However, the hydrophobicity of PCL causes cell attached difficulties which may limit clinical applications . Therefore, PEG and HA are good candidates for modifying the property of PCL for use as bone tissue engineering scaffolds.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and characterization of (PCL‐crosslinked‐PEG)/hydroxyapatite as bone tissue engineering scaffolds

Koupaei

Karkhaneh

Joupari

2015

J Biomedical Materials Res

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In this study, interconnected porous bioactive scaffolds were synthesized for bone tissue engineering. At the first step, poly( ɛ-caprolactone) (PCL) diols were diacrylated with acryloyl chloride. Then, the scaffolds were synthesized by radical crosslinking reaction of PCL and poly(ethyleneglycol) (PEG) diacrylates in the presence of hydroxyapatite (HA) particles. Morphological, swelling, thermal, and mechanical characteristics as well as degradability of the scaffolds were investigated. Results showed that increasing the ratio of PEG to PCL led to significant increase of swelling ratio and degradation rate, and decrease of crystallinity and compressive modulus of the networks, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an augmented crystallinity, a decreased swelling ratio, and also a significantly increased compressive modulus of the networks. Cytocompatability and osteoconductivity of the scaffolds were assessed by MTT and alkaline phosphatase (ALP) assays, respectively. The results confirmed the cytocompatible nature of PCL/PEG/HA scaffolds with no toxicity. MG-63 cells attached and spread on the pore walls offered by the scaffolds. PCL/PEG/HA scaffolds compared with PCL/PEG ones showed higher ALP activity. Thus, the results indicated that the PCL/PEG/HA scaffolds have the potential of being used as promising substrates in bone tissue engineering.

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“…With RP, scaffolds that closely match defect shapes can be constructed. Poly(ε-caprolactone) (PCL) has good biocompatibility and has been employed in the fused deposition modeling (a type of RP) of porous scaffolds [ 13 , 14 ]. However, because of the synthetic nature of PCL, the cells are not prone to adhere to and grow on the surface of PCL scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Healing of Osteochondral Defects Implanted with Biomimetic Scaffolds of Poly(ε-Caprolactone)/Hydroxyapatite and Glycidyl-Methacrylate-Modified Hyaluronic Acid in a Minipig

Hsieh

Shen

Wang

et al. 2018

IJMS

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Articular cartilage is a structure lack of vascular distribution. Once the cartilage is injured or diseased, it is unable to regenerate by itself. Surgical treatments do not effectively heal defects in articular cartilage. Tissue engineering is the most potential solution to this problem. In this study, methoxy poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) and hydroxyapatite at a weight ratio of 2:1 were mixed via fused deposition modeling (FDM) layer by layer to form a solid scaffold. The scaffolds were further infiltrated with glycidyl methacrylate hyaluronic acid loading with 10 ng/mL of Transforming Growth Factor-β1 and photo cross-linked on top of the scaffolds. An in vivo test was performed on the knees of Lanyu miniature pigs for a period of 12 months. The healing process of the osteochondral defects was followed by computer tomography (CT). The defect was fully covered with regenerated tissues in the control pig, while different tissues were grown in the defect of knee of the experimental pig. In the gross anatomy of the cross section, the scaffold remained in the subchondral location, while surface cartilage was regenerated. The cross section of the knees of both the control and experimental pigs were subjected to hematoxylin and eosin staining. The cartilage of the knee in the experimental pig was partially matured, e.g., few chondrocyte cells were enclosed in the lacunae. In the knee of the control pig, the defect was fully grown with fibrocartilage. In another in vivo experiment in a rabbit and a pig, the composite of the TGF-β1-loaded hydrogel and scaffolds was found to regenerate hyaline cartilage. However, scaffolds that remain in the subchondral lesion potentially delay the healing process. Therefore, the structural design of the scaffold should be reconsidered to match the regeneration process of both cartilage and subchondral bone.

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Biofabrication and in vitro study of hydroxyapatite/mPEG–PCL–mPEG scaffolds for bone tissue engineering using air pressure-aided deposition technology

Cited by 22 publications

References 26 publications

The mPEG-PCL Copolymer for Selective Fermentation of Staphylococcus lugdunensis Against Candida parapsilosis in the Human Microbiome

The mPEG-PCL Copolymer for Selective Fermentation of Staphylococcus lugdunensis Against Candida parapsilosis in the Human Microbiome

Preparation and characterization of (PCL‐crosslinked‐PEG)/hydroxyapatite as bone tissue engineering scaffolds

Healing of Osteochondral Defects Implanted with Biomimetic Scaffolds of Poly(ε-Caprolactone)/Hydroxyapatite and Glycidyl-Methacrylate-Modified Hyaluronic Acid in a Minipig

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