Biofabrication of microcapsules encapsulating beta-TC-6 cells via scalable device and in-vivo evaluation in type 1 diabetic mice

Bansal, Amit; D’Sa, Sucheta; D’Souza, Martin J.

doi:10.1016/j.ijpharm.2019.118830

Cited by 12 publications

(12 citation statements)

References 49 publications

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“…The mass loss might be caused by insufficient crosslinking of a small amount of copolymer molecular chains [ 39 , 40 ]. The finding was enough to demonstrate the excellent stability of the selected copolymer microcapsules [ 14 , 18 ]. Similar experimental results were obtained for other copolymer microcapsules with different diameters.…”

Section: Resultsmentioning

confidence: 99%

“…Three types of culture media were employed in this study: high-sugar medium containing 4.5 mg∙mL −1 glucose in solution, low-sugar medium containing 1.0 mg∙mL −1 glucose in solution, as well as sugar-free medium with no glucose in solution. High-sugar culture medium is often used for in-vitro cultivation of tumor cells, which is conducive to cell growth and proliferation [ 14 , 22 , 23 ]. Therefore, in this study, high-sugar culture medium was applied for routine culture of free and microencapsulated β-TC6 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the drug loading capacity of microcapsules is greatly increased but remains limited, so the exogenous insulin trapped inside cannot be released indefinitely. If insulin-producing cells can be packed into microcapsules, a steady supply of insulin will be guaranteed, which can provide long-term glucose-regulated insulin delivery [ 13 , 14 , 15 ]. The encapsulation systems can prevent cell leakage, protect encapsulated cells from immune attacks, as well as allow the diffusion of nutrients and the secretion of insulin [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Controlled Release of Insulin Based on Temperature and Glucose Dual Responsive Biomicrocapsules

Fan

Lei

et al. 2022

Molecules

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The treatment of diabetes lies in developing novel functional carriers, which are expected to have the unique capability of monitoring blood glucose levels continuously and dispensing insulin correctly and timely. Hence, this study is proposing to create a smart self-regulated insulin delivery system according to changes in glucose concentration. Temperature and glucose dual responsive copolymer microcapsules bearing N-isopropylacrylamide and 3-acrylamidophenylboronic acid as main components were developed by bottom-spray coating technology and template method. The insulinoma β-TC6 cells were trapped in the copolymer microcapsules by use of temperature sensitivity, and then growth, proliferation, and glucose-responsive insulin secretion of microencapsulated cells were successively monitored. The copolymer microcapsules showed favorable structural stability and good biocompatibility against β-TC6 cells. Compared with free cells, the biomicrocapsules presented a more effective and safer glucose-dependent insulin release behavior. The bioactivity of secreted and released insulin did not differ between free and encapsulated β-TC6 cells. The results demonstrated that the copolymer microcapsules had a positive effect on real-time sensing of glucose and precise controlled release of insulin. The intelligent drug delivery system is supposed to mimic insulin secretion in a physiological manner, and further provide new perspectives and technical support for the development of artificial pancreas.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Controlled Release of Insulin Based on Temperature and Glucose Dual Responsive Biomicrocapsules

Fan

Lei

et al. 2022

Molecules

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“…Furthermore, chitosan oligosaccharide has been applied for drug delivery system in diabetes. For instance, chitosan-microcapsulated insulin, chitosan-stabilized selenium nanoparticles, chitosan encapsulated resveratrol, chitosan coating of TiO 2 nanotube arrays for metformin, chitosan nanoparticle and chitosan hydrogel were used for improving diabetic therapy [134][135][136][137][138][139][140][141][142]. Interestingly, the development of chitosan oligosaccharide as a drug or supplement for diabetic treatment has been studied for a decade.…”

Section: Endocrinological Diseases and Diabetes Mellitusmentioning

confidence: 99%

Chitin and Chitosan Derivatives as Biomaterial Resources for Biological and Biomedical Applications

Satitsri

Muanprasat

2020

Molecules

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Chitin is a long-chain polymer of N-acetyl-glucosamine, which is regularly found in the exoskeleton of arthropods including insects, shellfish and the cell wall of fungi. It has been known that chitin can be used for biological and biomedical applications, especially as a biomaterial for tissue repairing, encapsulating drug for drug delivery. However, chitin has been postulated as an inducer of proinflammatory cytokines and certain diseases including asthma. Likewise, chitosan, a long-chain polymer of N-acetyl-glucosamine and d-glucosamine derived from chitin deacetylation, and chitosan oligosaccharide, a short chain polymer, have been known for their potential therapeutic effects, including anti-inflammatory, antioxidant, antidiarrheal, and anti-Alzheimer effects. This review summarizes potential utilization and limitation of chitin, chitosan and chitosan oligosaccharide in a variety of diseases. Furthermore, future direction of research and development of chitin, chitosan, and chitosan oligosaccharide for biomedical applications is discussed.

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“…12 Microcapsules, as scaffolds for cell attachment, proliferation and differentiation, are simple semipermeable membranes with superb properties regarding cell viability and inhibiting immune reaction. 12–14 Carboxymethyl cellulose (CMC) is a classic material used in bioengineering, not only was prepared as a compound scaffold but also was used to manufacture microcapsules via the co-flow microfluid technique because of its biocompatibility, water solubility and non-lethality toward cells. 13,15 Recently, studies in CMC microcapsules have focused on encapsulating growth factors or drugs instead of cells, but they face difficulties such as exploring release and short treatment periods.…”

Section: Introductionmentioning

confidence: 99%

Gene-modified BMSCs encapsulated with carboxymethyl cellulose facilitate osteogenesis in vitro and in vivo

et al. 2020

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Critical size bone defects are one of the most serious complications in orthopedics due to the lack of effective osteogenesis treatment. We fabricated carboxymethyl cellulose with phenol moieties (CMC-ph) microcapsules loaded with gene-modified rat bone mesenchymal stem cells (rBMSCs) that secrete hBMP2 following doxycycline (DOX) induction. The results showed that the morphology of microcapsules was spherical, and their diameters have equally distributed in the range of 100–150 μm; the viability of rBMSCs was unchanged over time. Through real-time PCR and Western blot analyses, the rBMSCs in microcapsules were found to secrete hBMP2 and to have upregulated mRNA and protein expression of osteogenesis-related genes in vitro and in vivo. Furthermore, the in vivo results suggested that the group with the middle concentration of cells expressed the highest amount of osteogenic protein over time. In this study, we showed that gene-modified rBMSCs in CMC-ph microcapsules had good morphology and viability. The BMP2–BMSCs/CMC-Ph microcapsule system could upregulate osteogenic mRNA and protein in vitro and in vivo. Further analysis demonstrated that the medium concentration of cells had a suitable density for transplantation in nude mice. Therefore, BMP2–BMSCs/CMC-Ph microcapsule constructs have potential for bone regeneration in vivo.

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Biofabrication of microcapsules encapsulating beta-TC-6 cells via scalable device and in-vivo evaluation in type 1 diabetic mice

Cited by 12 publications

References 49 publications

Controlled Release of Insulin Based on Temperature and Glucose Dual Responsive Biomicrocapsules

Controlled Release of Insulin Based on Temperature and Glucose Dual Responsive Biomicrocapsules

Chitin and Chitosan Derivatives as Biomaterial Resources for Biological and Biomedical Applications

Gene-modified BMSCs encapsulated with carboxymethyl cellulose facilitate osteogenesis in vitro and in vivo

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