Biofilm formation and antibiotic resistance pattern of dominant Staphylococcus aureus clonal lineages in China

Abstract

Biofilm formation usually increases antimicrobial resistance capacity of Staphylococcus aureus, which poses considerable challenges to successful eradication of staphylococcal infections. Very little is known about the biofilm-forming capacity and antibiotic resistance pattern of dominant S. aureus clonal lineages in China. In this study, the disk diffusion method and the microtiter plate assay were, respectively, performed to determine the antibiotic resistance and biofilm-forming capacity of 84 different S. aureus isolates belonging to 10 dominant clonal lineages in China. It was shown that isolates of different clonal lineages had obvious variations in biofilm-forming capacity. Resistance to individual antibacterial agents was most frequently observed to erythromycin (72.6%) and azithromycin (72.6%).Then, all tested isolates were further screened for the presence of 16 resistance genes by polymerase chain reaction (PCR), and no significant correlation was found between clonal lineage and the distribution of resistance genes. Overall, our results indicate that biofilm producers of S. aureus have a greater likelihood to carry more antibiotic resistance genes than non-biofilm producers (p < .01), further implying that biofilm may promote the dissemination of antibiotic resistance determinants. Practical applicationsStaphylococcus aureus is the leading cause of nosocomial infections and often associated with food poisoning worldwide. Biofilm formation of S. aureus influences the efficacy of antimicrobial therapy and promotes the persistence of infections; however, the knowledge about biofilm formation capacity of isolates belonging to different clonal lineages is little. Therefore, this study on biofilm formation capacity and antibiotic resistance pattern of dominant S. aureus clonal lineages could help to fix this information gap. K E Y W O R D Sagr type, antibiotic resistance, biofilm, clonal complex, Staphylococcus aureus

Biofilm formation of Staphylococcus aureus dairy isolates representing different genotypes

“…aureus (Tang et al, 2012;Lee et al, 2014). Biofilm formation can lead to persistent contamination or infection because the cells within the biofilm are very resistant to sanitation procedures and to the action of the host immune system and antimicrobial agents (Song et al, 2016). Different sources of Staph.…”

Diverse Profiles of Biofilm and Adhesion Genes in Staphylococcus Aureus Food Strains Isolated from Sushi and Sashimi

“…Sultan Zainal Abidin, Kuala Nerus, Terengganu, Malaysia. Direct inquiries to authors Puah and Chua (E-mail: suatmoi@um.edu.my & khchua@um.edu.my). it to cause infections (Foster, Geoghegan, Ganesh, & Hook, 2014). Moreover, S. aureus is a highly adaptable bacterium which has the ability to form structures known as biofilms among clinical and environmental strains (Song et al, 2017;Taj, Essa, Aziz, & Kazmi, 2012). According to Costerton, Stewart, and Greenberg (1999), biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix and adherent to an inert or living surface.…”

Genome Mining and Comparative Pathogenomic Analysis of An Endemic Methicillin-Resistant Staphylococcus Aureus (MRSA) Clone, ST612-CC8-t1257-SCCmec_IVd(2B), Isolated in South Africa

“…The isolates also harboured intercellular adhesion proteins (icaA, icaB, icaC, icaD, icaR) associated with attachment, proliferation and differentiation of micro-colonies into unique biofilm structures protecting them from the host defences such as antibodies or phagocytosis, making them extremely difficult to eradicate [54,55]. The association of dominant clonal lineages and biofilm formation has been reported in various studies on MRSA [56][57][58][59]. Naicker et al reported a strong biofilm formation in ST612 compared to the other clones which may have contributed to its dominance in South Africa [60].…”