Biofilm Formation and Antimicrobial Resistance in <i>Enterococcus</i>

Lee, Yangsoon

doi:10.3947/ic.2017.49.3.236

Cited by 14 publications

(8 citation statements)

References 9 publications

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“…The biofilm-forming ability is among the most important virulence properties of the Enterococcus [ 6 , 7 ]. Biofilm is a population of cells attached to various biotic and abiotic surfaces and is encapsulated in a hydrated matrix of exopolymeric substances, proteins, polysaccharides, and nucleic acids.…”

Section: Introductionmentioning

confidence: 99%

“…However, other studies suggest that the presence of esp is not necessary for the biofilm formation in E. faecium and E. faecalis strains [ 10 , 11 ]. Another protein reported to be involved in the biofilm formation is gelatinase (GelE), which can hydrolyze gelatin, collagen, and hemoglobin [ 6 , 12 ]. However, despite these reports, the authors demonstrated no association between biofilm formation and the presence of the gelE [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Biofilm Formation Capacity and Presence of Virulence Determinants among Enterococcus Species from Milk and Raw Milk Cheeses

Gajewska

Chajęcka‐Wierzchowska

Byczkowska-Rostkowska

et al. 2023

Life

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Bacterial biofilm is one of the major hazards facing the food industry. Biofilm-forming ability is one of the most important virulence properties of enterococci. The genus Enterococcus includes pathogenic, spoilage, and pro-technological bacteria. The presence of enterococci in milk and dairy products is usually associated with inadequate hygiene practices. The study examined the isolates’ capacity for biofilm formation and identification of the genetic determinants of its formation among 85 Enterococcus strains isolated from raw milk (n = 49) and soft-ripened cheeses made from unpasteurized milk (n = 36). E. faecalis and E. faecium were the dominant species. The obtained results showed that 41.4% isolates from milk and 50.0% isolates from cheeses were able to form biofilm. All of the isolates analyzed had at least one of the studied genes. As regards the isolates from raw milk, the most prevalent gene was the gelE (85.6%), followed by the asa1 (66.7%). None of the isolates from cheeses showed the presence of cylA and sprE. The most prevalent gene among the strains from this source was the epbC (94.4%), followed by the gelE (88.9%). In isolates from both sources, the presence of proteins from the Fsr group was noted the least frequently. Nevertheless, results showed that were no significant differences between the biofilm-producing Enterococcus spp. and non-biofilm-producing isolates in term of occurrences of tested virulence genes. The ability to produce a biofilm by enterococci isolated from raw milk or ready-to-eat products emphasizes the need for continuous monitoring of the mechanisms of microbial adhesion.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biofilm Formation Capacity and Presence of Virulence Determinants among Enterococcus Species from Milk and Raw Milk Cheeses

Gajewska

Chajęcka‐Wierzchowska

Byczkowska-Rostkowska

et al. 2023

Life

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“…In many cases, this trend is mediated by biofilm production in pathogens, which can increase the resistance of microbes to antibiotics and immune system responses. Biofilms are formed by the adhesion of a large number of bacteria to a contact surface, leading to the secretion of a mixture of polysaccharides and lipoproteins, enclosing the microbial colony and forming a membrane-like structure [ 2 ]. One notorious biofilm-forming pathogen is Staphylococcus aureus, a common hospital-acquired and food-borne bacterium [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Anti-Biofilm Activity of Pyrones from a Pseudomonas mosselii Strain

et al. 2022

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The emergence of drug resistant microbes over recent decades represents one of the greatest threats to human health; the resilience of many of these organisms can be attributed to their ability to produce biofilms. Natural products have played a crucial role in drug discovery, with microbial natural products in particular proving a rich and diverse source of antimicrobial agents. During antimicrobial activity screening, the strain Pseudomonas mosselii P33 was found to inhibit the growth of multiple pathogens. Following chemical investigation of this strain, pseudopyronines A-C were isolated as the main active principles, with all three pseudopyronines showing outstanding activity against Staphylococcus aureus. The analogue pseudopyronine C, which has not been well-characterized previously, displayed sub-micromolar activity against S. aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa. Moreover, the inhibitory abilities of the pseudopyronines against the biofilms of S. aureus were further studied. The results indicated all three pseudopyronines could directly reduce the growth of biofilm in both adhesion stage and maturation stage, displaying significant activity at micromolar concentrations.

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“…Among various oral microorganisms, Streptococcus mutans , Enterococcus faecalis, and Streptococcus gordonii are considered as major Gram-positive, facultative anaerobic dental pathogens predominantly found in the oral cavity of patients with apical periodontitis or dental caries ( Lee, 2017 ; Scharnow et al, 2019 ; Park et al, 2020 ). These dental pathogens can initiate and/or exacerbate the symptoms of the diseases by biofilm formation cooperating with other cariogenic or periodontopathic bacteria on root canal walls or dentin surfaces ( Abranches et al, 2018 ; Park et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria

Lee

Park

et al. 2021

Front. Microbiol.

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Bacterial biofilm residing in the oral cavity is closely related to the initiation and persistence of various dental diseases. Previously, we reported the anti-biofilm activity of Lactobacillus plantarum lipoteichoic acid (Lp.LTA) on a representative dental cariogenic pathogen, Streptococcus mutans. Since LTA structure varies in a bacterial strain-specific manner, LTAs from various L. plantarum strains may have differential anti-biofilm activity due to their distinct molecular structures. In the present study, we isolated Lp.LTAs from four different strains of L. plantarum (LRCC 5193, 5194, 5195, and 5310) and compared their anti-biofilm effects on the dental pathogens, including S. mutans, Enterococcus faecalis, and Streptococcus gordonii. All Lp.LTAs similarly inhibited E. faecalis biofilm formation in a dose-dependent manner. However, their effects on S. gordonii and S. mutans biofilm formation were different: LRCC 5310 Lp.LTA most effectively suppressed the biofilm formation of all strains of dental pathogens, while Lp.LTAs from LRCC 5193 and 5194 hardly inhibited or even enhanced the biofilm formation. Furthermore, LRCC 5310 Lp.LTA dramatically reduced the biofilm formation of the dental pathogens on the human dentin slice infection model. Collectively, these results suggest that Lp.LTAs have strain-specific regulatory effects on biofilm formation of dental pathogens and LRCC 5310 Lp.LTA can be used as an effective anti-biofilm agent for the prevention of dental infectious diseases.

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Biofilm Formation and Antimicrobial Resistance in Enterococcus

Cited by 14 publications

References 9 publications

Biofilm Formation Capacity and Presence of Virulence Determinants among Enterococcus Species from Milk and Raw Milk Cheeses

Biofilm Formation Capacity and Presence of Virulence Determinants among Enterococcus Species from Milk and Raw Milk Cheeses

Antibacterial and Anti-Biofilm Activity of Pyrones from a Pseudomonas mosselii Strain

Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria

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