Biofilm Formation and Antimicrobial Resistance Pattern of Uropathogenic E. Coli : Comparison of Phenotypic and Molecular Methods

Makled, Amal F.; Salem, Eman M.; Elbrolosy, Asmaa M.

doi:10.12816/0046227

Cited by 8 publications

(6 citation statements)

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“…Similar to our results, previous studies found that biofilm-producing UPEC isolates had a higher degree of antibiotic resistance than non-biofilm producing isolates [ 39 , 40 ]. Insufficient antibiotic concentration and/or their delayed penetration into the deeper layers of biofilms are the major reasons for antibiotic resistance in biofilm structures [ 17 , 18 ].…”

Section: Discussionsupporting

confidence: 92%

“…Insufficient antibiotic concentration and/or their delayed penetration into the deeper layers of biofilms are the major reasons for antibiotic resistance in biofilm structures [ 17 , 18 ]. Despite this, amikacin and nitrofurantoin showed high efficacy against biofilm producers, and could be considered as the antibiotics of choice for treatment of biofilm structures [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

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Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Karam

Habibi²,

Bouzari³

2018

Osong Public Health Res Perspect

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ObjectivesUropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.MethodsA total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.ResultsThe prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.ConclusionOur study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Karam

Habibi²,

Bouzari³

2018

Osong Public Health Res Perspect

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“… 28 According to a study by Makled et al, nitrofurantoin could be considered as selective antibiotics against biofilm structures. 73 Also, our finding suggests that nitrofurantoin was also effective against most of the biofilm producers. The findings could aid in the treatment of initial infection when biofilm is not formed in the isolates.…”

Section: Discussionmentioning

confidence: 60%

Antibiotic Resistance, Biofilm Formation and Sub-Inhibitory Hydrogen Peroxide Stimulation in Uropathogenic Escherichia coli

et al. 2022

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Uropathogenic Escherichia coli (UPEC) is the most prevalent cause of urinary tract infections (UTIs). Biofilm formation and antibiotic resistance could be high among the causative agent. The purpose of this study was to determine antibiotic resistance, biofilm production, and biofilm-associated genes, bcsA and csgD, and sub-inhibitory hydrogen peroxide (H2O2) stimulation in UPEC for biofilm formation. A total of 71 UPEC were collected from a tertiary care hospital in Kathmandu and subjected to identify antibiotic susceptibility using Kirby-Bauer disk diffusion. The biofilm formation was assessed using microtiter culture plate method while pellicle formation was tested by a tube method. In representative 15 isolates based on biofilm-forming ability, bcsA and csgD were screened by conventional polymerase chain reaction, and treated with sub-lethal H2O2. The UPEC were found the most susceptible to meropenem (90.2%), and the least to ampicillin (11.3%) in vitro and 90.1% of them were multi-drug resistant (MDR). Most UPEC harbored biofilm-producing ability (97.2%), and could form pellicle at 37°C. Among representative 15 isolates, csgD was detected only among 10 isolates (66.67%) while bcsA gene was present in 13 isolates (86.67%). This study revealed that level of biofilm production elevated after sub-lethal H2O2 treatment ( P = .041). These findings suggested that the pathogens are emerging as MDR. The biofilm production is high and the majority of selected strains contained bcsA and csgD genes. Pellicle formation test was suggestive to be an alternative qualitative method to screen biofilm production in UPEC. The sub-inhibitory concentration of H2O2 may contribute in increasing biofilm formation in UPEC.

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“…The 96-well flat polystyrene plates were used to test the biofilm inhibitory activity of compound OM1 and AuNPs-OM1 against four clinical microorganisms, including Gram-positive bacteria ( Staphylococcus aureus and Bacillus subtilis ) and Gram-negative bacteria ( Pseudomonas areuginosa and Escherichia coli ) [ 61 ]. In brief, each well was filled with 180 µL of lysogeny broth (LB broth) and then inoculated with 10 µL of pathogenic bacteria, followed by the addition of 10 µL (final concentration of 250 µg/mL) of samples along with a control (without test sample).…”

Section: Methodsmentioning

confidence: 99%

LC/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities

El‐Ghorab

Behery

Abdelgawad

et al. 2022

Plants

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Origanum majoranum L. is a Lamiaceae medicinal plant with culinary and ethnomedical applications. Its biological and phytochemical profiles have been extensively researched. Accordingly, this study aimed to investigate the chemical composition and the antibacterial and antioxidant properties of O. majoranum high features, as well as to search for techniques for activity optimization. A metabolomics study of the crude extract of O. majoranum using liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC ± HR ± ESI ± MS) was conducted. Five fractions (petroleum ether, dichloromethane, ethyl acetate, n-butanol, and aqueous) were derived from the total extract of the aerial parts. Different chromatographic methods and NMR analysis were utilized to purify and identify the isolated phenolics (high features). Moreover, the antimicrobial, antibiofilm, and antioxidant activity of phenolics were performed. Results showed that metabolomic profiling of the crude extract of O. majoranum aerial parts revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the isolation and identification of seven high-feature compounds comprising two phenolic acids, rosmarinic and caffeic acids, one phenolic diterpene, 7-methoxyepirosmanol, in addition to four flavonoids, quercetin, hesperitin, hesperidin, and luteolin. On the other hand, 7-methoxyepirosmanol (OM1) displayed the most antimicrobial and antioxidant potential. Such a phenolic principal activity improvement seems to be established after loading on gold nanoparticles.

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Biofilm Formation and Antimicrobial Resistance Pattern of Uropathogenic E. Coli : Comparison of Phenotypic and Molecular Methods

Cited by 8 publications

References 0 publications

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Antibiotic Resistance, Biofilm Formation and Sub-Inhibitory Hydrogen Peroxide Stimulation in Uropathogenic Escherichia coli

LC/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities

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Biofilm Formation and Antimicrobial Resistance Pattern of Uropathogenic E. Coli : Comparison of Phenotypic and Molecular Methods

Cited by 8 publications

References 0 publications

Relationships between Virulence Factors and Antimicrobial Resistance among &lt;italic&gt;Escherichia coli&lt;/italic&gt; Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among &lt;italic&gt;Escherichia coli&lt;/italic&gt; Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Antibiotic Resistance, Biofilm Formation and Sub-Inhibitory Hydrogen Peroxide Stimulation in Uropathogenic Escherichia coli

LC/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities

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Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran