Biofilm formation of periodontal pathogens on hydroxyapatite surfaces: Implications for periodontium damage

Jaffar, Norzawani; Miyazaki, Toshiki; Maeda, Takeyasu

doi:10.1002/jbm.a.35827

Cited by 26 publications

(21 citation statements)

References 48 publications

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“…produced strong biofilms by which they gradually increased after 2 to 7 days of incubation (Adetunji and Isola 2011;Mueller et al 2007). A study by Jaffar et al (2016) demonstrated that Actinomycetemcomitans and Porphyromonas gingivalis produced biofilm from days two to seven, with differing attachment ability. The present study demonstrates that strong biofilms formed on day three.…”

Section: Resultsmentioning

confidence: 99%

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Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

et al. 2019

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Pseudomonas aeruginosa and Streptococcus pyogenes are the most common pathogens to humans and are able to form a biofilm following ineffective precautionary approach. Biofilm is defined as a surface-attached community of bacterium embedded in an extracellular matrix which leads to tremendous problems in the environment, among humans and animals. This study aims to investigate the ability of P. aeruginosa and S. pyogenes to form biofilms in 96-well plate before further study in antibiofilm will be done. Initially, the 96-well plate was added with 100 μl of overnight P. aeruginosa culture with optical density (OD) 0.1 and S. pyogenes culture with OD 0.05. The cultures were incubated for 7 days at 37°C to justify the formation of biofilm. Subsequently, stained blue biofilm was detached from the plate by using 95% ethanol. Biofilms were finally measured using a micro plate reader at 570 nm and were classified based on the adherence strength formula. P. aeruginosa and S. pyogenes biofilms strongly adhered to the plates on days three, four, five and six. Interestingly on day three, biofilms showed the highest formation. However, moderate biofilm formation onto the plates by both P. aeruginosa and S. pyogenes were observed on day two, but non-adherence was observed on days one and seven. Day three is the optimum cultivation period for P. aeruginosa and S. pyogenes to switch into a strong biofilm in microtiter plate and could be beneficial for antibiofilm experiments.

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Section: Resultsmentioning

confidence: 99%

“…To detach the biofilms, 100 μl of 95% ethanol was added into each well. The solubilized biofilm formations were finally measured by the micro plate reader (Tecan Infinite 200 PRO, Austria Gmbh) at the wavelength of 570 nm (Jaffar et al 2016). The experiments were performed in triplicate.…”

Section: Biofilm Assaymentioning

confidence: 99%

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

et al. 2019

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“…The study design is illustrated in Fig. S5 and biofilm formation was induced using a previously reported method 65 . The single-species biofilm model, instead of a multi-species biofilm, was used as a simplified model to evaluate various testing conditions as described below.…”

Section: Methodsmentioning

confidence: 99%

Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation

Nakamura

Shirato

Tenkumo

et al. 2019

Sci Rep

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Titanium dental implants have been successfully used for decades; however, some implants are affected by peri-implantitis due to bacterial infection, resulting in loss of supporting bone. This study aimed to evaluate the effect of an antimicrobial chemotherapy employing H2O2 photolysis—developed to treat peri-implantitis—on biofilm-contaminated titanium surfaces in association with osteoblastic cell proliferation on the treated surface. Titanium discs were sandblasted and acid-etched, followed by contamination with a three-species biofilm composed of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mitis. This biofilm model was used as a simplified model of clinical peri-implantitis biofilm. The discs were subjected to ultrasound scaling, followed by H2O2 photolysis, wherein 365-nm LED irradiation of the disc immersed in 3% H2O2 was performed for 5 min. We analysed proliferation of mouse osteoblastic cells (MC3T3-E1) cultured on the treated discs. Compared with intact discs, biofilm contamination lowered cell proliferation on the specimen surface, whereas H2O2 photolysis recovered cell proliferation. Thus, H2O2 photolysis can recover the degraded biocompatibility of biofilm-contaminated titanium surfaces and can potentially be utilised for peri-implantitis treatment. However, to verify the findings of this study in relation to clinical settings, assessment using a more clinically relevant multi-species biofilm model is necessary.

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“…The microplate is re-solubilized by 150μl of 95% ethanol. Then, plate was measured at 570 nm by a microtiter plate reader (7) . According the optical density of the samples were classified Mean OD value (> 0.240) as strong biofilm,(0.120 -0.240) as moderate biofilm and (< 0.120) as weak biofilm (8) .…”

Section: Biofilm Susceptibility Assaymentioning

confidence: 99%

“…After forming biofilms on 96-well plates previously described (7) . The minimum biofilm inhibitory concentration (MBIC) were measured.…”

Section: Determination Of Minimum Biofilm Inhibitory Concentration (Mmentioning

confidence: 99%

Study ndvB gene expression in Pseudomonas aeruginosa Producing Biofilm

2021

MLU

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Pseudomonas aeruginosa have ability to form biofilm, this biofilm responsible for wide infections and antibiotic resistance development. A novel mechanism to antibiotic resistance mechanisms utilized by biofilm represented by the presence of ndvB geneencodes a glucosyltransferase enzyme involved in the formation of this glucans, thisglucan mediated sequestration antibiotic away from their cellular targets. In this study was collected 145 samples from burns patients and identification by biochwmicaltsets and Vitek-2 compact. The studied the biofilm formation was measured using micro titer plate method we found that 49 isolates (100%) of P.aeruginosa were biofilm positive and measured minimal biofilm inhibitory concentration(MBIC) of biofilm P.aeruginosa positive biofilmused cefepime as antibiotic was tested using microtiter plate microdilution method (MBIC) values (1gm/ml).Molecular detection of ndvB gene was measured using PCR 100% of isolates were found to contain ndv B gene. Expression of ndvB gene was significantly high in biofilm isolates using sub-MBIC values of cefepime. Expression of ndvB gene was significantly high in biofilm isolates using glucose with concentration 1% and 2%. This study we concluded biofilm formation is an important trait of P.aeruginosa that isa cause of antibiotic resistance. ndvB gene expression responsible for the biofilm resistance mechanism in P. aeruginosa biofilms.

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Biofilm formation of periodontal pathogens on hydroxyapatite surfaces: Implications for periodontium damage

Cited by 26 publications

References 48 publications

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

Determination of optimum incubation time for formation of Pseudomonas aeruginosa and Streptococcus pyogenes biofilms in microtiter plate

Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titanium surfaces for subsequent osteoblastic cell proliferation

Study ndvB gene expression in Pseudomonas aeruginosa Producing Biofilm

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