Biofilm Structure, Behavior, and Hydrodynamics

Purevdorj-Gage, Laura; Stoodley, Paul

doi:10.1128/9781555817718.ch9

Cited by 19 publications

(21 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The influence of the model parameters on the patterns is investigated in more detail by means of the two-dimensional reduction. The evolution of the patterns with some parameters seems to follow qualitative trends experimentally observed by other groups [36,[39][40][41][42][43] and by ourselves [44]. However, the effect of mechanical processes like displacement of cell aggregates due to external forces, thought to be relevant in the dynamics of real biofilm patterns such as streamers, must yet be accounted for.…”

Section: Introductionmentioning

confidence: 59%

“…Available CA models already include a few bacterial mechanisms and activities in a reasonable way. However, the description of more complex mechanisms, such as microbe attachment to surfaces [29,30], quorum sensing to form biofilms [31,32], generation of EPS matrix [33][34][35], and interaction with the surrounding flow [36][37][38], remains unclear. The most appropriate model for a particular biofilm should take into account the specific bacteria, the environment in which the biofilm is formed, the parameters that can be fitted to experiments, and the predictions to be made [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biofilm growth on rugose surfaces

2012

View full text Add to dashboard Cite

A stochastic model is used to assess the effect of external parameters on the development of submerged biofilms on smooth and rough surfaces. The model includes basic cellular mechanisms, such as division and spreading, together with an elementary description of the interaction with the surrounding flow and probabilistic rules for extracellular polymeric substance matrix generation, cell decay, and adhesion. Insight into the interplay of competing mechanisms such as the flow or the nutrient concentration change is gained. Erosion and growth processes combined produce biofilm structures moving downstream. A rich variety of patterns are generated: shrinking biofilms, patches, ripplelike structures traveling downstream, fingers, mounds, streamerlike patterns, flat layers, and porous and dendritic structures. The observed regimes depend on the carbon source and the type of bacteria.

show abstract

Section: Introductionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Biofilm growth on rugose surfaces

2012

View full text Add to dashboard Cite

show abstract

“…There are several steps in the formation of bacterial biofilms: (i) transport, (ii) initial adhesion, (iii) substrate attachment, and (iv) microcolony formation (cell-cell adhesion), leading to mature biofilms consisting of cells and a surrounding exopolymer matrix ( Figure 1) [14,46,49]. The first step in biofilm formation consists of the transport of the organism to a solid surface.…”

Section: Attachment To Surfacesmentioning

confidence: 99%

“…Image structural analysis (ISA) also calculates three textural parameters: textural entropy, angular second moment, and inverse difference moment. Because ISA was designed to analyze largerscale biofilm patters in two-dimensional grayscale images, it is primarily used for the analysis of conventional or epifluorescence microscopy images [49].…”

Section: Image Structural Analysismentioning

confidence: 99%

Biofilms: At the Interface between Biophysics and Microbiology

et al. 2006

View full text Add to dashboard Cite

This article highlights the role of biophysical principles in biofilm growth and propagation in food environments, an area that is of increasing concern to food processors due to the high resistance of biofilms to conventional remediation methodologies. First, the general characteristics of biofilms are discussed including their structure and physiological characteristics. Transfer and propagation mechanisms consisting of attachment followed by growth and subsequent detachment are reviewed. General growth models that are currently used in laboratories focusing on biofilm research are compared and emerging characterization techniques are discussed. An overview over current practices and techniques to remediate biofilms in a variety of environments is given. Remediation techniques that are reviewed include application of sanitizers and detergents. Finally, future research needs are briefly summarized.

show abstract

“…Macrocolonies are formed. These mature objects will be eroded by the external hydrodynamic forces, resulting on a rich variety of morphologies [17] (mushrooms, ripples, streamers, etc.). After some time and once a critical bacterial population has been reached, quorum sensing mechanisms activate spreading mechanisms (rolling, rippling, darting, etc., see figure 2).…”

Section: Overall Approachmentioning

confidence: 99%

A Cellular Automata Model for Biofilm Growth

Rodriguez¹,

Carpio²,

Einarsson³

2014

Proceedings of 10th World Congress on Computational Mechanics

View full text Add to dashboard Cite

Abstract. Biofilms are aggregates of bacteria attached to surfaces, which are very adaptable to changes in the environment and survive under extreme conditions. These living structures are behind many problems in research and industry. Therefore there is an increasing interest in improving our understanding on biofilms to be able to control them, either designing protocols to destroy them when harmful or promoting their growth when beneficial.A bidimensional cellular automata model for biofilm development is proposed to study the biofilm behaviour as its key growth parameters vary. The model includes several metabolic and spreading mechanisms typical of bacteria: cell division and spreading, detachment mechanisms adapted to the flow and probabilistic rules for EPS matrix generation.Numerical simulations of the model reproduce a number of biofilm patterns observed in real experiments: ripples, streamers, mushroom networks and patches. The influence of the nutrient concentration and the type of flow on the evolution of the bacterial community is monitorized.Biofilm tends to cover the whole surface when enough nutrients are available. Erosion enhances the creation of holes in this cover and promotes a variety of geometric patterns. The survival of the colony and its final shape will depend on the balance between the main growth parameters.Large Reynolds numbers and poor nutrient sources promote the formation of flat, and thin biofilms. Decreasing the Reynolds number or increasing the nutrient and oxygen concentration enhance pattern formation.

show abstract

Biofilm Structure, Behavior, and Hydrodynamics

Cited by 19 publications

References 99 publications

Biofilm growth on rugose surfaces

Biofilm growth on rugose surfaces

Biofilms: At the Interface between Biophysics and Microbiology

A Cellular Automata Model for Biofilm Growth

Contact Info

Product

Resources

About