Biofilms and Device-Associated Infections

Donlan, Rodney M.

doi:10.3201/eid0702.010226

Cited by 1,257 publications

(924 citation statements)

References 32 publications

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“…Bacteria commonly involved include Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus viridans, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa. 6 There are various methods to detect biofilm production. These include the Tissue Culture Plate (TCP), 7 Tube method (TM), 8 Congo Red Agar method (CRA), 9 bioluminescent assay, 10 piezoelectric sensors, 11 and fluorescent microscopic examination.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different detection methods of biofilm formation in the clinical isolates

Hassan¹,

Usman²,

Kaleem³

et al. 2011

Braz J Infect Dis

219

199

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BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard microbiological procedures. Biofilm detection was tested by TCP, TM and CRA. Antibiotic susceptibility test of biofilm producing bacteria was performed by using the Kirby-Bauer disc diffusion technique according to CLSI guidelines. RESULTS: The TCP method was considered to be superior to TM and CRA. From the total of 110 clinical isolates, TCP method detected 22.7% as high, 41% moderate and 36.3% as weak or non-biofilm producers. We have observed higher antibiotic resistance in biofilm producing bacteria than non-biofilm producers. CONCLUSION: We can conclude from our study that the TCP method is a more quantitative and reliable method for the detection of biofilm forming microorganisms as compared to TM and CRA methods, and it can be recommended as a general screening method for detection of biofilm producing bacteria in laboratories

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Section: Introductionmentioning

confidence: 99%

Evaluation of different detection methods of biofilm formation in the clinical isolates

Hassan¹,

Usman²,

Kaleem³

et al. 2011

Braz J Infect Dis

219

199

View full text Add to dashboard Cite

show abstract

“…The formation of biofilm is a process in which microorganisms irreversibly adhere and grow on the surface, producing extracellular polymer that ease adherence and matrix formation. However, physical methods, such as mechanical cleansing or friction, or even ultrasound, are effective in assuring its removal, provided that it is adequately performed (17) .…”

Section: Discussionmentioning

confidence: 99%

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Padovani¹,

Graziano

Goveia³

2008

Rev. Latino-Am. Enfermagem

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“…The organisms most commonly isolated from catheter biofilms are Staphylococcus epidermidis, Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococcus faecalis [16].…”

Section: Microbiologymentioning

confidence: 99%

Catheter-related bloodstream infections: catheter management according to pathogen

Leonidou

Gogos

2010

International Journal of Antimicrobial Agents

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Biofilms and Device-Associated Infections

Cited by 1,257 publications

References 32 publications

Evaluation of different detection methods of biofilm formation in the clinical isolates

Evaluation of different detection methods of biofilm formation in the clinical isolates

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Catheter-related bloodstream infections: catheter management according to pathogen

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