Biofilms of pathogenic bacteria in pig industry

Kolchyk, O. V.; Buzun, A. I.; Paliy, Anatoliy; Nalivayko, L.I.; Chekan, Oleksandr; Grebenik, Natalia; Bondarenko, Irina; Ребенко, Г. І.; Kushnir, V.Yu.; Todorov, Nikolay

doi:10.15421/2020_189

Cited by 3 publications

(2 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only a scientifically grounded complex of veterinary, sanitary and technological measures ensures the conduct of animal husbandry at a high level (Tomley & Shirley, 2009;Shkromada et al, 2019a;Palii et al, 2020a). In turn, infectious (Stegniy et al, 2019;Kolchyk et al, 2020;Palii et al, 2020b) and parasitic (Majeed et al, 2015;Paliy et al, 2018b;Liu et al, 2020) animal diseases are a significant limiting factor in the development of many livestock industries. Invasive diseases cause tremendous harm to the animal's organism because parasites destroy organs and tissues, poison the whole body with their metabolic products, which cause hypovitaminosis, and weaken immunity, creating conditions for the occurrence of other diseases (Britton et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Study of the toxicity and effectiveness of an antiparasitic agent based on tinidazole and fenbendazole

Paliy¹,

Сумакова²,

Palii³

2020

Ukr J Ecol

View full text Add to dashboard Cite

The development of new import-substituting highly effective and environmentally friendly complex antiparasitic drugs and their implementation in a scientifically grounded system of measures to combat the causative agents of the main invasive diseases of farm animals and poultry remains an urgent task of veterinary medicine. Based on the results of studies of anthelmintic and antiprotozoal action of agents on exogenous forms of parasites, an experimental form of a complex antiparasitic agent "Tinidafen" was created and investigated. An experimental sample in the form of a powder was created on the basis of two active substances – tinidazole and fenbendazole with the addition of excipients (talc, starch). It has been established that the LD50 of "Tinidafen" for white rats with a single dose is 2564.36±349.13 mg/kg. The therapeutic dose of "Tinidafen" for rabbits is 7.5 g per 10 kg of live body weight. The extensefectevity of "Tinidafen" is 99.5% for helminths and 98.7% for eimeria. After administered orally of the agent, helminth eggs in the feces of infested animals were not registered from day 3, and the release of eimeria oocysts was observed until day 5 of the experiment.

show abstract

Section: Introductionmentioning

confidence: 99%

Study of the toxicity and effectiveness of an antiparasitic agent based on tinidazole and fenbendazole

Paliy¹,

Сумакова²,

Palii³

2020

Ukr J Ecol

View full text Add to dashboard Cite

show abstract

“…Паралельно, у тих же господарствах, упродовж 4 років також дослідили 637 проб зерна кормового ячменю (по 70-120 г зерна кожна проба) у 37 партіях зразків, відібраних з бурту/годівниці методом «конверту». Через можливу присутність у пробах зерна мікробних біоплівок, їхні екстракти досліджували у розведенні 1:30-1:50 [11].…”

unclassified

Conception of the 'feed’s chain' for porcine circovirus-bacterial infections in piggery

Buzun

Kolchyk

Muzyka

et al. 2021

Veterinary Medicine: Inter-Departmental Subject Scientific Collection

View full text Add to dashboard Cite

The obtained experimental-analytical data indicate a significant risk of occurrence of circovirus-associated syndrome complexes proliferative necrotic pneumonia and a complex of respiratory diseases of pigs (PNP and PRDC) from the presence of Pasteurella and Pasteurellacea bacteria in barley grain (OR = 3.48; 2.21 < OR < 5.50; p ≤ 0.01 at n = 633) and the critical importance of these bacteria for the clinical manifestation of these syndromes in pig farms of Ukraine, which are seropositive both for PCV-2 and AD (OR = 35.45; 18.12 < OR < 69.35; p ≤ 0.001 at n = 433). Thus, barley grain grown on forage lands “fertilized” with pig manure is a factor in the transmission of at least pasteurellae in the epizootic chains of PNP and PRDC, and thus form a “feed chain” of PCV-2. Prospects for further development of the concept of the food chain of circovirus-associated infections in pig breeding are discussed

show abstract

Microorganisms’ Growth Inhibition in Poultry Meat Using Bacillus spp.

Кolchyk,

Borovuk,

Buzun

et al. 2024

WVJ

View full text Add to dashboard Cite

Meat processing enterprises are currently seeking ways to improve the efficiency of their operations. This study aimed to assess the presence of harmful microorganisms in poultry meat treated with a probiotic complex of Bacillus spp. bacteria during storage. Of the 2,516 meat samples collected from broiler chickens across six poultry processing enterprises in the Dnipropetrovsk region over three years, 1,845 samples tested positive for pathogens. Listeria spp. were isolated in 52.7% of meat samples, S. aureus in 28.7%, P. aeruginosa – in 6.9%, E. coli in 4.2%, and Salmonella spp. in 7.5%. The next stage of the study was the infection of 10 samples of poultry meat with pathogens of test cultures (Escherichia coli UNCSM - 007, Pseudomonas aeruginosa UNCSM - 012, Staphylococcus aureus UNCSM - 017, Listeria ivanovii UNCSM - 042, Salmonella Enteritidis UNCSM - 081), followed by aerosol treatment with a probiotic complex of Bacillus spp. (1.5×108 in ml (0.5 Mac Farland) administered at a dose of 1 ml per sample with daily registration of colony growth. Following pathogen contamination and a single aerosol treatment with the probiotic complex of Bacillus spp., the growth of E. coli and S. aureus was already suppressed on the second day of meat storage. The probiotic complex of Bacillus spp. was able to displace Salmonella Enteritidis on the third day and P. aeruginosa on day 4, but the growth of L. ivanovii could be observed only on day 5. The probiotic complex of Bacillus spp. formed visible biofilms from the five strains of microorganisms and remained viable for five days, forming a dense biofilm with a high accumulation rate of 4.73 D620. A distinctly noticeable ability to form microbial biofilms within three days was observed in planktonic forms of L. ivanovii up to 2.88 D620, followed by P. aeruginosa at 2.28 D620. Low biofilm density was observed for Salmonella Enteritidis (1.77 D620) and S. aureus (1.76 D620). The probiotic complex of bacteria of the genus Bacillus spp. shows potential for use in meat processing plants to prevent the growth of harmful microbial biofilms on meat products stored under refrigeration.

show abstract

Biofilms of pathogenic bacteria in pig industry

Cited by 3 publications

References 45 publications

Study of the toxicity and effectiveness of an antiparasitic agent based on tinidazole and fenbendazole

Study of the toxicity and effectiveness of an antiparasitic agent based on tinidazole and fenbendazole

Conception of the 'feed’s chain' for porcine circovirus-bacterial infections in piggery

Microorganisms’ Growth Inhibition in Poultry Meat Using Bacillus spp.

Contact Info

Product

Resources

About