Biofunctionalization of surfaces using polyelectrolyte multilayers

Hartmann, Hanna; Krastev, Rumen

doi:10.1515/bnm-2016-0015

Cited by 16 publications

(20 citation statements)

References 86 publications

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“…Antiadhesive properties depend on surface morphology or mechanical properties, in turn bactericidal effect is maintained by the presence of an active antibacterial component. 17,52 Our results suggested that all LbL coatings have shown anti- LbL multilayers. The bactericidal activity of these films could be associated with the presence of silver nanoparticles in the upper layer.…”

Section: Discussionmentioning

confidence: 61%

“…The bacterial adhesion to the different substrates is dependent on the component composition and their physicochemical properties, for instance, stiffness, hydrophilicity, topography, and so on. 1,17 Antibacterial activity of LbL coatings can be associated with their antiadhesive and bactericidal properties. Antiadhesive properties depend on surface morphology or mechanical properties, in turn bactericidal effect is maintained by the presence of an active antibacterial component.…”

Section: Discussionmentioning

confidence: 99%

“…It has been established that enhancement of surface hydrophilicity led to reduction of bacterial adhesion. 1 Hartmann and Krastev as well as Fang et al 1,17 showed that negatively charged surfaces repealed bacterial attachments due to their similar charges. Stiffness of the film is also one of the key factors determining bacterial adhesion.…”

mentioning

confidence: 99%

“…Stiffness of the film is also one of the key factors determining bacterial adhesion. 17,22 At the same time, different biocide agents can be incorporated in LbL assemblies with subsequent releasing and elimination of bacteria. For instance, charged antibiotics (such as gentamicin, levofloxacin, nisin, etc.)…”

mentioning

confidence: 99%

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Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential

Kulikouskaya

Zhdanko

Hileuskaya

et al. 2021

J Biomedical Materials Res

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Implant-related infection is one of the serious problems in regenerative medicine.Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly. In this study, multilayer films based on biopolymers (pectin and chitosan) and their nanocomposites with silver nanoparticles have been prepared and evaluated. The buildup of multilayers was monitored using the quartz crystal microbalance with dissipation technique. The morphology of the obtained films was investigated by atomic force microscopy. We have demonstrated that pectin-Agcontaining films were characterized by the linear growth and smooth defect-free surface. When pectin-Ag was substituted for the pectin in the multilayer systems, the properties of the formed coatings were significantly changed: the film rigidity and surface roughness increased, as well as the film growth acquired the parabolic character. All prepared multilayer films have shown antibacterial activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.The significant decrease in the number of the adhered E. coli on the multilayer surface has been determined; moreover, many of the cells were misshapen with cytoplasm leaking. The prepared multilayer films showed a mild activity against S. aureus predominantly due to the antiadhesive effect. Our results indicate that antibacterial activity of biopolymer multilayers is determined by the film composition and physicochemical characteristics and can be associated with their antiadhesive and bactericidal behaviors.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential

Kulikouskaya

Zhdanko

Hileuskaya

et al. 2021

J Biomedical Materials Res

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“…Within the current issue, Hartmann and Krastev [1] outline specific strategies using polyelectrolyte multilayers to modulate these interactions between biomaterial surfaces and biological systems. Biofunctionalization is one particular form of surface modification involving the immobilization of biomolecules as proteins, peptides and polysaccharides or bioactive drugs with the same purpose.…”

Section: Doi: 101515/bnm-2017-0007mentioning

confidence: 99%

Biofunctionalization

Petersen

2017

BioNanoMaterials

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Much effort is invested in the novel design and synthesis of biomaterials for the fabrication of biomedical applications as implants or nanoparticles with appropriate functionality, mechanical properties and durability. Depending on the application, requirements might differ considerably, ranging from high mechanical stress resistance to high transparency. These functions are generally governed by the bulk composition of the biomaterial. The biological response is in contrast largely controlled by the surface chemistry and structure. The rationale for surface modification of polymers is therefore straightforward: retaining the key physical properties of a biomaterial while modifying only the outermost surface to influence the biointeraction. Commonly observed interactions of any material with a biological system or system containing biomolecules cover adsorption or adhesion processes of proteins and bacteria or platelets as well as phagocytosis and fibrous encapsulation. Effective surface modification of biomaterials should mediate these interactions, for example, with the purpose of improved tissue-interface related-biocompatibility, that is especially important for modern implant design, which aim to improve implant integration while avoiding chronic inflammation and foreign body reactions, and thus loss of the intended implant function.Within the current issue, Hartmann and Krastev [1] outline specific strategies using polyelectrolyte multilayers to modulate these interactions between biomaterial surfaces and biological systems. Biofunctionalization is one particular form of surface modification involving the immobilization of biomolecules as proteins, peptides and polysaccharides or bioactive drugs with the same purpose. Various immobilization methods are available while the chosen strategy/design significantly defines the biological activity of the functionalized biomaterial. In this context, Spatz et al. [2] discuss the impact of spacer integration between biomaterial surface and the integrin-recognition motif cyclic RGD on the formation of integrin-based cellular adhesion. Furthermore, the immobilization strategy defines the short-term or long-term localization of the biomolecule on the biomaterials surface, which is explored using the example of the immobilization of the potent angiogenic vascular endothelial growth factor (VEGF) in order to improve the hemocompatibility and endothelialization of biodegradable polymer surfaces [3].One more important field is the biofunctionalization of nanostructured materials and nanoparticles with possible biomedical application for imaging and quantifying of target molecules such as proteins in assays, cells and tissues. In this context, Walter et al. [4] give general insights into the principles and factors controlling the binding affinity of aptamers, as promising alternative binders that can substitute antibodies in various applications immobilized to nanostructured materials. The short review by Salehi [5] additionally summarizes current designs of different biofu...

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Nanoarchitectonics for Free‐Standing Polyelectrolyte Multilayers Films: Exploring the Flipped Surfaces

et al. 2022

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The competency of polyelectrolyte multilayer films (PEM) self-assembly to modulate the surface architecture at the molecular level has emerged as a captivating technique for various biomedical applications. PEM advancement in bioapplications covered the development of bio-sensors, stimuliresponsive drug-delivery systems, surface modification for creating antibacterial surfaces, and tissue-engineering architectures. Different PEM blends, from natural to synthetic ones, have been explored to make a free-standing version of the PEM stack. This review provides a deeper understanding of the free-standing PEM (FS-PEM), their fabrication strategies, and essential properties for specified bio-applications. The most crucial aspect of fabricating FS-PEM is choosing an application-specific fabrication protocol to delaminate the PEM stack from the underlying substrates. Post-fabrication, there are important considerations to improve the mechanical stability of these FS-PEMs by crosslinking or adding desired nanoparticles. Overall, essential insights are discussed here for using FS-PEM for specific bio-application, which has received little attention in the material science community until now.

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Biofunctionalization of surfaces using polyelectrolyte multilayers

Cited by 16 publications

References 86 publications

Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential

Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential

Biofunctionalization

Nanoarchitectonics for Free‐Standing Polyelectrolyte Multilayers Films: Exploring the Flipped Surfaces

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