Biogeneration of Silica Nanoparticles from Rice Husk Ash Using Fusarium oxysporum in Two Different Growth Media

Abstract

The biotransformation of rice husk ash (RHA) by Fusarium oxysporum to generate silica nanoparticles was carried out using two different commercial growth media: malt-glucose (MG) and malt-glucose-yeast-peptone (MGYP). Biomass production, substrate consumption, organic acids production, and solubilized silica were measured during RHA biotransformation. Extracellular proteins were analyzed by SD-PAGE. Silica nanoparticles were analyzed by XRD, zeta potential, SEM, and TEM. The results showed that the production of organic acids was not directly related to the solubilization of silica. Solubilization and stabilization of silica occur mainly in the exponential growth phase of F. oxysporum, which are associated with the action of extracellular proteins with sizes 24, 55, and 70 kDa. MG medium presented the best performance for the growth of F. oxysporum and production of semicrystalline, quasi-spherical silica nanoparticles in the range of 2−8 nm.

Shape and Size Diversity of Gold, Silver, Selenium, and Silica Nanoparticles Prepared by Green Synthesis Using Fungi and Bacteria

“…The possibility of silica nanoparticle formation with fungal cultures has been barely studied. In addition to our research, , there have been a number of reports describing the synthesis of silica spheres and quasispheres by the ascomycete Fusarium oxysporum. − Calcination of such quasispherical, highly crystalline silica nanoparticles by bioleaching of rice husk resulted in the loss of occluded protein and in the formation of porous, often cubic, structures …”

From E-Waste to High-Value Materials: Sustainable Synthesis of Metal, Metal Oxide, and MOF Nanoparticles from Waste Printed Circuit Boards

“…Numerous groups of fungi are employed for the synthesis of nanoparticles from WPCBs, with Fusarium oxysporum emerging as a prominent organism capable of producing a variety of manufactured nanoparticles. The synthesis of silver nanoparticles (AgNPs) by F. oxysporum is primarily dependent on a reductase associated with NADH [ 4 , 112 ].…”