Bioinformatics advances in protein ubiquitination

Lu, Liang; Liu, Dong; He, Fuchu

doi:10.3724/sp.j.1005.2013.00017

Cited by 12 publications

(10 citation statements)

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“…They were then identified through analysis of their morphological characteristics under a microscope to confirm that they were all Ae. aegypti 33 . The first generation of artificially reared female adults (F1) was bioassayed for resistance and compared with pyrethroid‐sensitive adult Ae.…”

Section: Methodsmentioning

confidence: 99%

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Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province

Wang,

Zhang,

Gao

et al. 2024

Pest Management Science

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BackgroundAedes aegypti is a main vector of arboviral diseases, principally dengue, chikungunya, and Zika. Insecticides remain the most effective vector control method. Pyrethroid is the main insecticides currently used, and the long‐term use of insecticides can cause mosquitoes to develop knockdown resistance. Studying the mutation sites and genotypes of Ae. aegypti can reveal the mutation characteristics and regional distribution of the kdr gene in the Ae. aegypti population. Testing for a correlation between the mutation rate in various populations and pyrethrin resistance can clarify the resistance mechanism.ResultsThe bioassay results showed that all 15 populations are resistant populations. In the study of the kdr gene, three non‐synonymous mutations were identified in the DNA of F1s from the wild Ae. aegypti population: S989P (TCC‐CCC), V1016G (GTA‐GGA), and F1534C (TTC‐TGC). The mortality rate of various populations was correlated with the mutation rate at the V1016G + F1534C locus but not with that at the S989P + V1016G locus.ConclusionAe. aegypti populations in border areas of Yunnan Province are resistant to permethrin and beta‐cyfluthrin. The insecticidal effect of beta‐cyfluthrin is stronger than that of permethrin. The mutation rate at sites V1016G + F1534C is negatively correlated with the mortality of Ae. aegypti based on bioassay.This article is protected by copyright. All rights reserved.

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Section: Methodsmentioning

confidence: 99%

“…aegypti. 33 The first generation of artificially reared female adults (F1) was bioassayed for resistance and compared with pyrethroid-sensitive adult Ae. aegypti from Hainan Province, China, which have been raised in the laboratory for more than 10 years and have never been exposed to insecticides.…”

Section: Mosquito Samplingmentioning

confidence: 99%

Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province

Wang,

Zhang,

Gao

et al. 2024

Pest Management Science

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“…Research has shown that the UPS is crucial for protein degradation in cells and that it can destroy up to 80%–85% of proteins in eukaryotic cells ( Amm et al, 2014 ). When a ubiquitin tag is added to a substrate, it can alter the substrate’s function, localization, protein interaction, or stability and then regulate many different cellular life activities ( Lu et al, 2013 ; Streich Jr and Lima, 2014 ). These activities are crucial to cell growth.…”

Section: The Ubiquitin-proteasome System (Ups) Pathwaymentioning

confidence: 99%

Ferroptosis and the ubiquitin-proteasome system: exploring treatment targets in cancer

Din,

Lin,

Wang

et al. 2024

Front. Pharmacol.

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Ferroptosis is an emerging mode of programmed cell death fueled by iron buildup and lipid peroxidation. Recent evidence points to the function of ferroptosis in the aetiology and development of cancer and other disorders. Consequently, harnessing iron death for disease treatment has diverted the interest of the researchers in the field of basic and clinical research. The ubiquitin-proteasome system (UPS) represents a primary protein degradation pathway in eukaryotes. It involves labelling proteins to be degraded by ubiquitin (Ub), followed by recognition and degradation by the proteasome. Dysfunction of the UPS can contribute to diverse pathological processes, emphasizing the importance of maintaining organismal homeostasis. The regulation of protein stability is a critical component of the intricate molecular mechanism underlying iron death. Moreover, the intricate involvement of the UPS in regulating iron death-related molecules and signaling pathways, providing valuable insights for targeted treatment strategies. Besides, it highlights the potential of ferroptosis as a promising target for cancer therapy, emphasizing the combination between ferroptosis and the UPS. The molecular mechanisms underlying ferroptosis, including key regulators such as glutathione peroxidase 4 (GPX4), cysteine/glutamate transporter (system XC-), and iron metabolism, are thoroughly examined, alongside the role of the UPS in modulating the abundance and activity of crucial proteins for ferroptotic cell death, such as GPX4, and nuclear factor erythroid 2–related factor 2 (NRF2). As a pivotal regulatory system for macromolecular homeostasis, the UPS substantially impacts ferroptosis by directly or indirectly modulating iron death-related molecules or associated signaling pathways. This review explores the involvement of the UPS in regulating iron death-related molecules and signaling pathways, providing valuable insights for the targeted treatment of diseases associated with ferroptosis.

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“…The ubiquitin proteasome pathway (UPP), a major intracellular system for ATP-dependent extra-lysosomal protein degradation in the cell cycle, mediates the degradation of 80–85% of intracellular proteins. It consists of ubiquitin, ubiquitin-activating enzymes E1, ubiquitin-conjugating enzymes E2, ubiquitin ligases E3, proteasome and deubiquitinating enzyme [ 16 ]. Numerous studies have demonstrated that UPP plays critical roles in controlling the levels of various cellular proteins, thereby regulating basic cellular processes such as cell division and growth, apoptosis, signal transduction, DNA damage and repair, immune response, and so on [ 17 , 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression Profiles of Sp-uchl3 and Sp-uchl5 during Gonad Development of Scylla paramamosain

et al. 2018

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Ubiquitin C-terminal hydrolases (UCHLs) are a subset of deubiquitinating enzymes, and are involved in numerous physiological processes. However, the role of UCHLs during gonad development has not been studied in crustaceans. In this study, we have first cloned and analyzed expression profiling of Sp-uchl3 and Sp-uchl5 genes from mud crab Scylla paramamosain. The full-length cDNA of Sp-uchl3 is of 1804 bp. Its expression level in the ovary was significantly higher than in other tissues (p < 0.01), and during gonadal development, its expression in both O1 and O5 stages was significantly higher than in the other three stages of ovaries (p < 0.05), while in T3 it was higher than in the former two stages of testes (p < 0.05). Meanwhile, the full-length cDNA of Sp-UCHL5 is 1217 bp. The expression level in the ovary was significantly higher than in other tissues (p < 0.01). Its expression in ovaries was higher than in testes during gonadal development (p < 0.05). The expression level in the O5 stage was the highest, followed by the O3 stage in ovarian development, and with no significant difference in the testis development (p > 0.05). These results provide basic data showing the role of Sp-UCHL3 and Sp-UCHL5 in the gonad development of the crab.

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Bioinformatics advances in protein ubiquitination

Cited by 12 publications

References 0 publications

Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province

Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province

Ferroptosis and the ubiquitin-proteasome system: exploring treatment targets in cancer

Molecular Characterization and Expression Profiles of Sp-uchl3 and Sp-uchl5 during Gonad Development of Scylla paramamosain

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