Biolistic transformation of prokaryotes: factors that affect biolistic transformation of very small cells

Smith, F. D.; Harpending, Peter R.; Sanford, Jay P.

doi:10.1099/00221287-138-1-239

Cited by 66 publications

(30 citation statements)

References 27 publications

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“…on top of a plastic platform in a glass petri plate with Drierite desiccant) to dry. Exposure to high humidity during or after drying may result in rapid water absorption by the ethanol, resulting in clumping of particles and tight (sometimes irreversible) binding to the macrocarrier, and dramatically reduced transformation rates (Smith et al 1992). Loaded macrocarriers should be used as soon as possible.…”

Section: Preparation Of Microcarriers and Coating With Dnamentioning

confidence: 99%

The Biolistic® PDS-1000/He device

Kikkert

1993

Plant Cell Tiss Organ Cult

111

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This paper describes the design, operation, and performance of the Biolistic ® PDS-1000/He device, which is used to transform living organisms with foreign DNA. DNA is delivered to cells in association with microscopic metal particles, called microcarriers, that are propelled at high velocity towards target tissues. The microcarriers are accelerated on a plastic cylinder, called a macrocarrier, which is driven by a shock wave of helium gas. The effectiveness of the PDS-1000/He device was tested by bombarding tobacco cell suspension cultures with microcarriers that were coated with plasmid DNA containing the B-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII) genes. Two days after bombardment, there were 6835-594 cell clusters per petri plate that expressed the GUS gene. Kanamycin resistant colonies were observed 6 to 8 weeks after bombardment, at a rate of 838 ---134 colonies per bombarded plate.

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Section: Preparation Of Microcarriers and Coating With Dnamentioning

confidence: 99%

The Biolistic® PDS-1000/He device

Kikkert

1993

Plant Cell Tiss Organ Cult

111

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“…This has been successfully applied to many species, in particular those recalcitrant species difficult to transform by other means, such as most monocotyledons and some dicotyledons. There are also reports stating the use of this methodology for the genetic transformation of fungi, bacteria and animal cells (Johnston et al 1991;Smith et al 1992;Toffaletti et al 1993).…”

Section: Particle Bombardmentmentioning

confidence: 95%

Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don

Zárate

Verpoorte

2007

Phytochem Rev

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This review describes the different plant transformation techniques, including guided infection with Agrobacterium tumefaciens and A. rhizogenes, particle bombardment and protoplast fusion, that have been attempted to create transgenic Catharanthus roseus (L.) G. Don cell cultures, hairy roots and whole plants. The review also focuses on the different approaches used to manipulate and improve secondary metabolite yields in various culture systems, with special attention to the most relevant results achieved. Finally, under future perspectives, the authors propose several approaches which would likely be implemented with this species, to try to boost the accumulation of the anti-tumour agents, vinblastine and vincristine. Some comments on how the future of the genetic manipulation of medicinal plants may proceed aiming at achieving higher secondary metabolite yields are also given.

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“…We used a PDS-1000/He particle delivery system (Bio-Rad, California, USA) to transform the mitochondria and chloroplast, following the standard procedures designed for bacteria (Smith et al, 1992). …”

Section: Conditions For Biolistic Bombardmentmentioning

confidence: 99%

Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii

et al. 2005

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Three types of respiratory deficient mitochondrial strains have been reported in Chlamydomonas reinhardtii: a deficiency due to (i) two base substitutions causing an amino acid change in the apocytochrome b (COB) gene (i.e., strain named dum-15), (ii) one base deletion in the COXI gene (dum-19), or (iii) a large deletion extending from the left terminus of the genome to somewhere in the COB gene (dum-1, -14, and -16). We found that these respiratory deficient strains of C. reinhardtii can be divided into two groups: strains that are constantly transformable and those could not be transformed in our experiments. All transformable mitochondrial strains were limited to the type that has a large deletion in the left arm of the genome. For these mitochondria, transformation was successful not only with purified intact mitochondrial genomes but also with DNA-

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Biolistic transformation of prokaryotes: factors that affect biolistic transformation of very small cells

Cited by 66 publications

References 27 publications

The Biolistic® PDS-1000/He device

The Biolistic® PDS-1000/He device

Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don

Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii

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