The Biolistic® PDS-1000/He device

Kikkert, Julie R.

doi:10.1007/bf02319005

Cited by 111 publications

(42 citation statements)

References 8 publications

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“…Transient expression of GhVIN1-GFP and GhVIN1-RFP fusion proteins in onion (Allium cepa) epidermal cells was performed with a PDS-1000/He Biolistic particle-delivery system (Bio-Rad) as described by Kikkert (1993). GFP-and RFP-expressing cells were detected with a confocal laser scanning microscope (Zeiss LSM 510 META) with argon laser excitation wavelengths of 488 nm (GFP) and 543 nm (RFP).…”

Section: Subcellular Localization Of Ghvin1-gfp or Ghvin1-rfpmentioning

confidence: 99%

Evidence That High Activity of Vacuolar Invertase Is Required for Cotton Fiber and Arabidopsis Root Elongation through Osmotic Dependent and Independent Pathways, Respectively

Wang

Li²,

Lian³

et al. 2010

Plant Physiology

169

136

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Vacuolar invertase (VIN) has long been considered as a major player in cell expansion. However, direct evidence for this view is lacking due, in part, to the complexity of multicellular plant tissues. Here, we used cotton (Gossypium spp.) fibers, fast-growing single-celled seed trichomes, to address this issue. VIN activity in elongating fibers was approximately 4-6-fold higher than that in leaves, stems, and roots. It was undetectable in fiberless cotton seed epidermis but became evident in initiating fibers and remained high during their fast elongation and dropped when elongation slowed. Furthermore, a genotype with faster fiber elongation had significantly higher fiber VIN activity and hexose levels than a slow-elongating genotype. By contrast, cell wall or cytoplasmic invertase activities did not show correlation with fiber elongation. To unravel the molecular basis of VIN-mediated fiber elongation, we cloned GhVIN1, which displayed VIN sequence features and localized to the vacuole. Once introduced to Arabidopsis (Arabidopsis thaliana), GhVIN1 complemented the short-root phenotype of a VIN T-DNA mutant and enhanced the elongation of root cells in the wild type. This demonstrates that GhVIN1 functions as VIN in vivo. In cotton fiber, GhVIN1 expression level matched closely with VIN activity and fiber elongation rate. Indeed, transformation of cotton fiber with GhVIN1 RNA interference or overexpression constructs reduced or enhanced fiber elongation, respectively. Together, these analyses provide evidence on the role of VIN in cotton fiber elongation mediated by GhVIN1. Based on the relative contributions of sugars to sap osmolality in cotton fiber and Arabidopsis root, we conclude that VIN regulates their elongation in an osmotic dependent and independent manner, respectively.

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Section: Subcellular Localization Of Ghvin1-gfp or Ghvin1-rfpmentioning

confidence: 99%

Evidence That High Activity of Vacuolar Invertase Is Required for Cotton Fiber and Arabidopsis Root Elongation through Osmotic Dependent and Independent Pathways, Respectively

Wang

Li²,

Lian³

et al. 2010

Plant Physiology

169

136

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“…Initially, the gun powder discharge device was used to accelerate inert metal microprojectiles coated with biologically active compounds [21]. Soon after, this device was replaced with inert gas helium (Biolistics1 PDS-1000/He) to offer the force for microprojection [22]. The rupture disk assembly is the major part of this most commonly used device which controls the helium pressure and helium gas released by a rupture disc and partial vacuum to propel a macrocarrier plastic sheet loaded with DNA coated tungsten or gold.…”

Section: Microprojectile Bombardmentmentioning

confidence: 99%

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

Rashid¹,

Lateef²

2016

AJPS

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From the early past to the present, biotechnologies have produced the ability to genetically transform a wide variety of plant species. The plant transformation technologies have changed the face of agriculture and plant biology. Plant genetic transformation is one of the key technologies for crop improvement in addition to emerging approach for producing recombinant proteins in plants. Both plastid genomes and plant nuclear can be genetically modified. Until now, essential functional differences between the prokaryotic-like genome of the plastid and the eukaryotic genome of the plant cell nucleus will have an impact on characteristics of transgenic organism. Thus, the main goals are to generate transgenic plants with the traits of interest as well as minimizing the amount of transgenic DNA in plants while maximizing stability of gene expression and trait performance. In this review, two broad groups of gene delivery methods will be discussed namely, (bilogical and physical methods) and subsequently there applications for improving disease resistance will be discussed.

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“…The plasmid DNA was amplified in Escherichia coli, strain HB101, and extracted using Wizard Miniprep DNA Purification System (Promega, Madison,WI, USA). Five µg of vector DNA was bound to 3 mg gold particles (BIO-RAD, Hercules, CA, USA) following the procedure described by Kikkert (1993). The PDS-1000/He Biolistic Particle Accelerator (BIO-RAD, Hercules, CA, USA) was used for gene transfer.…”

Section: Bacterial Strains and Biolistic Transformationmentioning

confidence: 99%

“…However, pressure higher than 94.9 kg cm -2 caused a decline in the efficiency of transformation. Higher pressure may damage the calli due to severe conditions, acoustic and gas shocks (Kikkert 1993) rendering them unable to grow.…”

Section: Effect Of Helium Pressure On Bombardment Efficiencymentioning

confidence: 99%

Genetic transformation of Stella De Oro daylily by particle bombardment

Aziz¹,

Sauvé²,

Zhou³

2003

Can. J. Plant Sci.

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S. 2003. Genetic transformation of Stella De Oro daylily by particle bombardment. Can. J. Plant Sci. 83: 873-876. Daylily (Hemerocallis sp. 'Stella de Oro') callus cultures initiated from ovules were bombarded with gold particles coated with plasmid harboring Basta ® resistance gene. Resulting putative transgenic calli were selected after 3 wk on semi-solid Murashige and Skoog's (MS) basal medium supplemented with 10 mg L -1 1-naphthaleneacetic acid, 2 mg L -1 6-benzylaminopurine and 3 mg L -1 phosphinothricin (PPT). Surviving calli regenerated shoots after 2 mo on semi-solid MS medium supplemented with 2 mg L -1 thiadiazuron and 1 mg L -1 PPT. Polymerase chain reaction and Southern blotting were used to confirm independent transformation events.

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The Biolistic® PDS-1000/He device

Cited by 111 publications

References 8 publications

Evidence That High Activity of Vacuolar Invertase Is Required for Cotton Fiber and Arabidopsis Root Elongation through Osmotic Dependent and Independent Pathways, Respectively

Evidence That High Activity of Vacuolar Invertase Is Required for Cotton Fiber and Arabidopsis Root Elongation through Osmotic Dependent and Independent Pathways, Respectively

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

Genetic transformation of Stella De Oro daylily by particle bombardment

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