Biologic Data of Cynomolgus Monkeys Maintained under Laboratory Conditions

Rosso, Marilena Caterina; Badino, P.; Ferrero, Giulio; Costa, Rosane Dias; Cordero, Francesca; Steidler, Stephanie

doi:10.1371/journal.pone.0157003

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…Previous study also reported that the mean levels of ALP concentrations were significantly higher in boys than girls, indicating that similar changes in ALP was also reported in humans [27]. In addition, sex-related difference in GGT levels have been demonstrated in cynomolgus monkeys [28]. Likewise, we observed that the concentrations of ALP and GGT were significantly higher in males than in females in both cynomolgus and rhesus monkeys aged between 48 and 96 months.…”

Section: Discussionsupporting

confidence: 89%

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

et al. 2019

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Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters in cynomolgus and rhesus monkeys are limited. In the present study, we established hematologic and biochemical reference intervals for healthy cynomolgus and rhesus monkeys anesthetized with ketamine hydrochloride. A total of 142 cynomolgus monkeys (28 males and 114 females) and 42 rhesus monkeys (22 males and 20 females) were selected and analyzed in order to examine reference intervals of 20 hematological and 16 biochemical parameters. The effects of sex were also investigated. Reference intervals for hematological and biochemical parameters were separately established by species (cynomolgus and rhesus) and sex (male and female). No sex-related differences were determined in erythrocyte-related parameters for cynomolgus and rhesus monkey housed in indoor laboratory conditions. Alkaline phosphatase and gamma glutamyltransferase were significantly lower in females than males in both cynomolgus and rhesus monkeys aged 48-96 months. The reference values for hematological and biochemical parameters established herein might provide valuable information for researchers using cynomolgus and rhesus monkeys in experimental conditions for biomedical studies.

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Section: Discussionsupporting

confidence: 89%

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

et al. 2019

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“…30,31 Furthermore, cynomolgus monkeys are well established in previous literature as scientifically appropriate subjects for such studies. 32,33 Non-naïve animals were included as recipients in this study due to limited subject availability and to most ethically and prudently steward the use of research animals. These animals had previously been involved in pharmacokinetic studies that involved administration of drugs intended to reduce relapsed alcohol consumption and for treatment of cocaine abuse.…”

Section: Xenotransplant Recipientsmentioning

confidence: 99%

Immunological response in cynomolgus macaques to porcine α‐1,3 galactosyltransferase knockout viable skin xenotransplants—A pre‐clinical study

Holzer¹,

Chang²,

Wicks³

et al. 2020

Xenotransplantation

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Background: Allogeneic skin recovered from human deceased donors (HDD) has been a mainstay interim treatment for severe burns, but unfortunately risk of infectious disease and availability limitations exist. Genetically engineered ɑ-1,3 galactosyltransferase knockout (GalT-KO) porcine source animals for viable skin xenotransplants may provide a promising clinical alternative. Methods: Four cynomolgus macaque recipients received full-thickness surgical wounds to model the defects arising from excision of full-thickness burn injury and were treated with biologically active skin xenotransplants derived from GalT-KO, Designated Pathogen Free (DPF) miniature swine. Evaluations were conducted for safety, tolerability, and recipient immunological response. Results: All skin xenotransplants demonstrated prolonged survival, vascularity, and persistent dermal adhesion until the study endpoint at post-operative day 30. No adverse outcomes were observed during the study. Varying levels of epidermolysis coincided with histologic detection of CD4+ and CD8+ T cells, and other cellular infiltrates in the epidermis. Recipient sera IgM and IgG demonstrated significant antibody immune response to non-α-1,3-galactose porcine xenoantigens. Separately, specific wound healing mediators were quantified. Neither porcine cell migration nor PERV were detected in circulation or any visceral organs. Conclusions: These results provide a detailed analysis of vital skin xenotransplants utilizing a non-human primate model to predict the anticipated immunological response of human patients. The lack of adverse rejection even in the presence of elevated Ig indicates this is a prospective therapeutic option. The findings reported here directly supported regulatory clearance for a first-in-man, Phase I xenotransplantation clinical trial.

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“…6,8 However, the result in this study agrees with the reports that ALP serum levels were significantly higher in adult males than in the adult females. 2,5,8 One young mature male monkey, with body weight of ies on cynomolgus monkeys also reported ALP values higher than 1000 IU/L. 8,13,14 The serum of monkeys also appeared yellowish suggestive of the free bilirubin circulating in the blood.…”

Section: Discussionmentioning

confidence: 99%

Correlations of serum biochemistry profile with ultrasonic histogram of liver, gallbladder, and kidneys and morphometry of rescued long‐tailed macaques (Macaca fascicularis)

Cardenio

Acorda

Lastica-Ternura

2020

J of Medical Primatology

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Diagnostic tools are infrequently used in assessing health conditions of rescued wildlife such as the long-tailed macaques in local wildlife rehabilitation and rescue centers in the Philippines. Health assessment of recently surrendered and rescued long-tailed macaques is limited to physical examination. Diagnostic tools such as serum biochemistry and diagnostic imaging are not readily present and performed upon arrival of new monkeys and during regular general health checkup of long-tailed macaques. These diagnostic tools are important in evaluating the general health condition of the animal, disease diagnosis, and monitoring. Serum evaluation is infrequently performed in rescued monkeys and is limited only to those being kept in laboratories. Serum biochemistry values for laboratory-kept long-tailed macaques originated from Mauritius, 1 China, 2 and Cambodia 3 were previously reported. Sex-related differences were observed on serum biochemistry values of healthy male and female macaques in previous studies. 4-8 The differences in serum biochemistry values observed in monkeys from a different country of origin might have been caused by their exposure to different dietary constituents and environment. Moreover, many factors including climate, genetic background, and age of the animals could affect the results. 4 In addition, increase or

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Biologic Data of Cynomolgus Monkeys Maintained under Laboratory Conditions

Cited by 17 publications

References 32 publications

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Reference values of hematological and biochemical parameters in young-adult cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta) anesthetized with ketamine hydrochloride

Immunological response in cynomolgus macaques to porcine α‐1,3 galactosyltransferase knockout viable skin xenotransplants—A pre‐clinical study

Correlations of serum biochemistry profile with ultrasonic histogram of liver, gallbladder, and kidneys and morphometry of rescued long‐tailed macaques (Macaca fascicularis)

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