Biologic Markers for Odontogenic Periradicular Periodontitis

Abstract

Introduction The diagnosis and assessment of apical periodontitis by traditional periapical radiographs can be challenging and might yield false-negative results. The aim of this study was to determine whether interleukin-1beta (IL-1β) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) can be used as biological markers for apical periodontitis. Methods Forty healthy patients with teeth diagnosed with apical periodontitis of pulpal origin were included in the study. GCF samples were obtained from the diseased tooth and from a healthy contralateral control tooth. Total protein concentration in each sample was determined by using the Bio-Rad protein assay. Enzyme-linked immunosorbent assay was used to analyze the concentration of IL-1β and DSP in the samples. Results Protein content of the GCF was statistically significantly higher in the disease group compared with the control group. The levels of IL-1β and DSP were not statistically different between disease and control groups. Conclusions Although this study was unable to demonstrate a significantly higher level of IL-1β or DSP in the GCF of teeth with apical periodontitis, the observed presence of a significantly higher level of total protein in the GCF of diseased teeth suggests the possible role of total protein level as a marker for periapical disease.

Genetic Predisposition to Persistent Apical Periodontitis

“…The results of this study are not in agreement with other studies (35, 36). Siqueira et al (35) reported little to no association with polymorphism and posttreatment apical periodontitis.…”

Correlation between Clinical/Radiographic Features and Inflammatory Cytokine Networks Produced by Macrophages Stimulated with Endodontic Content

“…Research works involving the ccomprehension of apical periodontitis mechanisms and the developing of clinical and radiographic signs have emerged in the endodontic literature (39)(40)(41)(42)(43)(44). Morsani et al (41) suggested that the specific genetic markers associated with increased IL-1b production might contribute to apical periodontitis increased susceptibility.…”

T-Lymphocyte and Cytokine Expression in Human Inflammatory Periapical Lesions

“…The increased production of IL-1b has been detected in periapical lesions (6,18), although some researchers have noticed the levels of this cytokine were not statistically different between diseased teeth and healthy control teeth (11,19). In this study when bacterial load of the infected root canal was diminished at day 7, the levels of mRNA expression of IFN-g, IL-1b, and RANKL were significantly decreased (P < .05), whereas the levels of TNF-a and IL-17A mRNA expression were maintained.…”