Biologic properties of molecularly cloned and expressed murine interleukin-3

Abstract

Interleukin-3 (IL-3) (multipotential colony-stimulating factor [multi- CSF]) is an important regulator of hemopoiesis. We recently isolated a cDNA clone of this gene and describe in this manuscript the biologic properties of the expressed gene product. An SV40 expression vector carrying cDNA encoding murine interleukin-3 was constructed so that expression of the IL-3 gene was placed under the control of the SV40 early promoter. When the expression vector was transfected to COS-1 monkey cells, IL-3 activity was secreted into the medium, reaching maximal levels 72 hours after transfection. The IL-3 produced by the COS-1 cells was partially purified using diethylaminoethyl Sephacel and phenyl-Sepharose, and its chromatographic properties were the same as IL-3 produced by the WEHI-3 cell line. The biologic activities of the “expressed” IL-3 include the “induction”of 20-alpha-hydroxysteroid dehydrogenase (20-alpha-SDH) in splenic lymphocytes from nu/nu mice, proliferative activity for 32D cl-23 and FDC-P1 cell lines, and colony- stimulating activity for granulocyte-macrophage, eosinophil, megakaryocyte, natural killer-like, erythroid, and multipotential colony-forming cells from murine fetal liver and adult bone marrow.

Recombinant gibbon interleukin‐3 stimulates megakaryocyte colony growth in vitro from human peripheral blood progenitor cells

“…A murine T cell-derived lymphokine, interleukin-3 (IL-3), has recently been purified, cloned, and demonstrated to have a broad range of effects on murine hematopoiesis including megakaryocytopoiesis (Ihle et al, 1982(Ihle et al, , 1983Hapel et al, 1985;Spivak et al, 1985;Quesenberry et al, 1985). Among its activities, murine IL-3 augments erythroid burst colony formation and stimulates in vitro colony growth by granulocyte, macrophage, megakaryocyte, and multipotential hematopoietic progenitor cells (Ihle et al, 1982(Ihle et al, , 1983Hapel et al, 1985;Quesenberry et al, 1985). Murine IL-3 appears t o be the active factor in WEHI-3 conditioned media responsible for stimulating megakaryocyte colony growth in rodents but accounts for only part of the activity in pokeweed mitogen spleen cell conditioned medium (Quesenberry et al, 1985).…”

Binding of iodinated multipotential colony‐stimulating factor (interleukin‐3) to murine bone marrow cells

“…Mr 23,000 produced by activated T-lymphocytes, which has multiple biological activities on murine hemopoietic cells (Iscove et al, 1982;Ihle et al, 1983;Cutler et al, 1985;Hapel et al, 1985;Metcalf et al, unpublished), It stimulates the survival and probably proliferation of some classes of multipotential hemopoietic stem cells, colony formation in agar cultures by multipotential progenitor cells, and progenitor cells committed to the granulocyte, macrophage, eosinophil, megakaryocyte, erythroid, and mast cell lineages and is required for the survival and proliferation of some immortalized myeloid and mast cell lines in vitro.…”

Pancreatic carcinoma associated with marked eosinophilia: A case report

“…Recent advances in bone marrow culture studies have disclosed that several hemopoietic growth factors stimulate eosino-phi1 production. In mouse, eosinophil differentiation factor (EDF), Eo-CSF, granulocytemacrophage CSF (GM-CSF) and interleukin 3 (IL 3) are involved in the production of eosinophils in vitro (19)(20)(21)(22). Recently murine EDF was shown to be identical to murine Eo-CSF (21) and B-cell growth factor I1 (BCGF 11, (23)).…”