Biological activities of salvianolic acid B from<i>Salvia miltiorrhiza</i>on type 2 diabetes induced by high-fat diet and streptozotocin

Huang, Mingqing; Wang, Peijian; Xu, Shuyu; Xu, Wen; Chu, Kedan; Lu, Jin‐Jian

doi:10.3109/13880209.2014.959611

Cited by 55 publications

(37 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sal B is a typical bioactive compound isolated from Danshen. A recent study indicated that Sal B can inhibit symptoms of DM in rats and these effects may partially be correlated with its insulin sensitivity, glycogen synthesis, and antioxidant activity (Huang et al 2015). However, there is little about possible signaling pathway of Sal B on DM.…”

Section: Discussionmentioning

confidence: 99%

Protection of SAL B with H9C2 cells

Sun

Zuo

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Salvianolic acid B (Sal B) is regarded as a potent antidiabetic agent and has been reported to possess cardioprotective effect in vivo. Objective: This study investigated the cardioprotective effects of Sal B on H9c2 cells injury caused by high glucose in vitro, and clarified the possible mechanisms. Materials and methods: Di ferent concentrations of Sal B were incubated with cells for 12 h prior being exposed to high glucose for 24 h. Cardioprotective effects of Sal B were evaluated using CCK-8 assay, ELISA, Hoechst 33258 nucleus staining, and western blot. Results: Following a 24 h exposure of H9c2 to high glucose, obvious reduction was found in cell viability (45%), GSH (54.8 ± 9.4 ng/mg protein), catalase (1.22 ± 0.12 U/mg protein), and GPX level (67.9 ± 9.4 U/mg protein), which were associated with the increases of GSSG (1.99 ± 0.28 ng/mg protein) and ROS (2.00 ± 0.19 RFU/mg protein) production. High glucose also elevated IL-6 (1.8-fold), IL-1b (1.9-fold), and TNF-a (1.6-fold) level, as well as induced cell apoptosis and NF-kB (6.1-fold) activation. However, Sal B (25 and 50 mM) elevated cell viability (28% and 44%), ameliorated oxidative stress (GSH, 1.3-and 1.6-fold; catalase, 1.9-and 2.0-fold; GPX, 1.1-and 1.4-fold; GSSG, 0.9-and 0.8-fold; ROS, 0.6-and 0.5-fold), and inflammatory response (IL-6, 0.9-and 0.7-fold; IL-1b, 0.8-and 0.6-fold; TNF-a, 0.9-and 0.8-fold), and inhibited cell apoptosis and NF-kB (0.5-and 0.2-fold) expression. Conclusion: Sal B attenuated high glucose-induced injury and cytotoxicity through inhibiting inflammatory cytokine production in H9c2 cardiac cells. ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 99%

Protection of SAL B with H9C2 cells

Sun

Zuo

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…There are many approaches to efficiently decrease blood glucose level such as promoting insulin secretion [24], inhibiting glucose absorption [25], stimulating glucose uptake by tissues [26] and increasing insulin sensitivity [27]. Insulinotropic agents such as glibenclamide, stimulate insulin secretion and dramatically decrease blood glucose level in both normal and diabetic animals.…”

Section: Discussionmentioning

confidence: 99%

Daidzin decreases blood glucose and lipid in streptozotocin-induced diabetic mice

Tian¹,

Liu²,

Liu³

et al. 2016

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…Although its most common use is in the treatment of cardio-cerebral vascular diseases [14][15][16], Sal B is recently reported to possess a number of potential implications in the treatment of insulin resistance, obesity, and type 2 diabetes [17][18][19][20]. Sal B or Salvia miltiorrhiza extract improved glycemic control, dyslipidemia, and insulin sensitivity in high fat diet and streptozocin-induced type 2 diabetic rats [17,19]. Furthermore, Sal B can moderate lipids and reduce obesity in high-fat diet-induced obese C57BL/6J mice by suppressing peroxisome proliferator-activated receptor gamma (PPARγ)-mediated adipogenesis [18].…”

Section: Introductionmentioning

confidence: 99%

Salvianolic Acid B Ameliorates Hyperglycemia and Dyslipidemia in db/db Mice through the AMPK Pathway

Huang

Zhou

Zhang

et al. 2016

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: Salvianolic acid B (Sal B), a major polyphenolic compound of Salvia miltiorrhiza Bunge, has been shown to possess potential antidiabetic activities. However, the action mechanism of SalB in type 2 diabetes has not been investigated extensively. The present study was designed to investigate the effects of Sal B on diabetes-related metabolic changes in a spontaneous model of type 2 diabetes, as well as its potential molecular mechanism. Methods: Male C57BL/KsJ-db/db mice were orally treated with Sal B (50 and 100 mg/kg) or metformin (positive drug, 300 mg/kg) for 6 weeks. Results: Both doses of Sal B significantly decreased fasting blood glucose, serum insulin, triglyceride and free fatty acid levels, reduced hepatic gluconeogenic gene expression and improved insulin intolerance in db/db mice. High dose Sal B also significantly improved glucose intolerance, increased hepatic glycolytic gene expression and muscle glycogen content, and ameliorated histopathological alterations of pancreas, similar to metformin. Sal B treatment resulted in increased phosphorylated AMP-activated protein kinase (p-AMPK) protein expression in skeletal muscle and liver, increased glucose transporter 4 (GLUT4) and glycogen synthase protein expressions in skeletal muscle, and increased peroxisome proliferator-activated receptor alpha (PPARα) and phosphorylated acetyl CoA carboxylase (p-ACC) protein expressions in liver. Conclusion: Our data suggest that Sal B displays beneficial effects in the prevention and treatment of type 2 diabetes at least in part via modulation of the AMPK pathway.

show abstract

Biological activities of salvianolic acid B fromSalvia miltiorrhizaon type 2 diabetes induced by high-fat diet and streptozotocin

Cited by 55 publications

References 25 publications

Protection of SAL B with H9C2 cells

Protection of SAL B with H9C2 cells

Daidzin decreases blood glucose and lipid in streptozotocin-induced diabetic mice

Salvianolic Acid B Ameliorates Hyperglycemia and Dyslipidemia in db/db Mice through the AMPK Pathway

Contact Info

Product

Resources

About