Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Forman, Marjorie L.; Inhorn, Stanley L.; Sheaff, Edmund; Cherry, James D.

doi:10.3181/00379727-131-34040

Cited by 3 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An adequate population is assured by the ability of both cell lines to progress through at least 50 doublings before senescence. Thus, growth potential of these lines is equal to or greater than that reported for human diploid cells (11) and rhesus monkey diploid cells (12). The doubling time of both cell lines (approximately 30 hours) com- 3 DECEMBER 1971 pares favorably with other values found for primate lung cells (2,13).…”

supporting

confidence: 52%

Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Petricciani

Hopps

Lorenz

1971

Science

View full text Add to dashboard Cite

show abstract

supporting

confidence: 52%

Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Petricciani

Hopps

Lorenz

1971

Science

View full text Add to dashboard Cite

show abstract

“…Diploid or continuous cell lines established from simian kidneys have been somewhat less sensitive than primary or secondary cells for isolation of human viruses. 8 ' 11 ' 30 Human fetal diploid lung and kidney cell lines were found to be far more sensitive than primary or secondary monkey kidney cells for isolation of rhinoviruses, and early studies indicated that human fetal diploid cell lines were highly sensitive for propagation and isolation of other human viruses as well. 7 - 13 > 31 For the past 8 years, clinical specimens examined in this laboratory for the presence of virus have been inoculated into both primary or secondary rhesus monkey kidney (MK) cells and human fetal diploid (HFD) cells (either lung or kidney, depending upon the type of specimen).…”

Section: Comparative Sensitivities Of Certain Cellmentioning

confidence: 99%

“…8 '33 Schmidt NJ, Lennette EH: Complement-fixing and fluorescent antibody responses to an attenuated rubella virus vaccine. Am J Epidemiol 91:351-354, 1970 34.Schmidt NJ, Lennette EH: Comparison of various methods for preparation of viral serological antigens from infected cell cultures.…”

mentioning

confidence: 99%

Tissue Culture in the Laboratory Diagnosis of Viral Infections

Schmidt

1972

Am J Clin Pathol

View full text Add to dashboard Cite

Schmidt, Nathalie J.: Tissue culture in the laboratory diagnosis of viral infections. Am. J. Clin. Pathol. 57: 820-828, 1972. The use of both rhesus monkey kidney and human fetal diploid cell cultures for attempts to isolate virus from human clinical specimens recovered approximately 30% more isolates from fecal, throat, skin lesion, and tissue specimens than were recovered in a single cell culture system. The sensitivities of the two cell types for isolation of viruses in the major groups infecting man are compared. The use of both BS-C-1 and RK-13 cells yielded approximately 25% more rubella virus isolates than were recovered in a single cell system. Procedures such as the establishment of cell cultures from tissues suspected of harboring viruses, cocultivation of cells from biopsy or autopsy specimens with other cell types, and the use of organ cultures, have in certain situations resulted in greater sensitivity for recovery of viruses. Micro cell culture tests have been devised for virus identification and antibody assays; these are far more economical than conventional tests in terms of reagents, space requirements, and personnel time. Methods have been developed for the production of higher-titered viral serologic antigens in cell culture systems. IN THE PAST TWO DECADES the use of in vitrocell cultures in diagnostic virology has resulted in the discovery of almost 200 viruses which infect man. Cell cultures have also provided a practical and economical host system which, in many instances, may be substituted for animals or embryonated eggs in viral isolation attempts, neutralization tests, or preparation of serologic antigens.Cell culture media, suspensions of viable cells, and prepared cell cultures are now available commercially for laboratories

show abstract

“…Previous reports on the serial culture of cells from kidney tissues of an AG monkey (5), rhesus monkey fetus (6), and a marmoset monkey (7) suggested the feasibility of establishing diploid cell lines from tissues of subhuman primates.…”

mentioning

confidence: 99%

Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production

Wallace¹,

Vasington²,

Petricciani

et al. 1973

In Vitro

View full text Add to dashboard Cite

Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Cited by 3 publications

References 5 publications

Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Subhuman Primate Diploid Cells: Possible Substrates for Production of Virus Vaccines

Tissue Culture in the Laboratory Diagnosis of Viral Infections

Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production

Contact Info

Product

Resources

About