BIOLOGICAL CONTROL OF POTATO SOFT ROT DISEASE (Erwinia carotovora) USING Bacillus subtilis AND Pseudomonas fluorescens

Abstract

Potato (Solanum tuberosum L.) is one of the commodities of potential as a source of carbohydrates and important on Indonesia's economy. One of the constraints of potato production is soft rot disease caused by Erwinia carotovora (syn. Pectobacterium carotovorum) which is needed to be controlled. One alternative control measures is the used of bacteria Bacillus subtilis and Pseudomonas fluorescens for biological control. The purpose of this research is to know the potential of B. subtilis and P. fluorescens in single and in combination to inhibit potato soft rot diseases caused by E. carotovora. The results showed that biological control agents B. subtilis and P. fluorescens in single or in combination both of them (T3 to T17) have potential in inhibiting the growth of pathogenic E. carotovora by showing the clear zone. Biological agents provides nearly the same effect if compared with the bactericide Agrept (T2). Based on the results of the statistical analysis, the treatment 11 th and treatment 14 th showed a clear zone wider than the Agrept treatment (T2). The treatment 11 th contain combination of biological agents B. subtilis (10 9 cfu/ml) and P.fluorescens (10 5 cfu/ml), while the treatment 14 th contain combination of biological agents B.subtilis (10 7 cfu/ml) and P. fluorescens (10 5 cfu/ml).

Impact of silver nanoparticles and two biological treatments to control soft rot disease in sugar beet (Beta vulgaris L)

“…Carotovora. Many Bacillus species have proved to be effective against a broad range of plant pathogens (Bacon et al 2001;Cook et al 2002;Deng et al 2011;Shafi et al 2017 andIstiqomah andToyibah (2013). Figure 5 shows that the induction of polyphenol oxidase (PPO) in the first application date after 81 days was significantly increased with all applied treatments compared to control (13 mg/min).…”

“…Bacillus species have become attractive biological control agents due to their ability to produce hard, resistant endospores and antibiotics which control a broad range of plant pathogens (Cavaglieri et al 2005;Deng et al 2011). Biological control agent B. subtilis (10 9 cfu/ml) has potential in inhibiting the growth of pathogenic E. carotovora by showing the clear zones (Istiqomah and Toyibah 2013). The objective of the present study was to the possible use of silver nanoparticles, Spirulina platensis and B. subtilis, to evaluate their efficacy against sugar beet soft rot caused by P. carotovora, as a possible new trend for disease control.…”