Biological control of Sclerotinia sclerotiorum (Lib.) de Bary with Trichoderma harzianum and Bacillus amyloliquefaciens

Bio-suppression of Sclerotinia Stem Rot of Tomato and Biostimulation of Plant Growth Using Tomato-associated Rhizobacteria

“…However, few attempts have been made to explore the potential use of bacterial biocontrol agents for the management of Sclerotinia diseases [12,15,17]. In the present study, 25 bacterial strains recovered from rhizospheric soils around healthy tomato plants and belonging to Bacillus, Chryseobacterium, Enterobacter, and Klebsiella genera were assessed for their ability to suppress Sclerotinia Stem Rot disease and to promote tomato growth.…”

“…Biocontrol is eco-friendly, safe and may provide long-term protection to the crop. Reduced Sclerotinia Stem Rot incidence and severity have been demonstrated in numerous studies and successful disease control was achieved using fungi [10][11][12][13][14], bacteria [7,12,[15][16][17] or biofungicides [18][19][20] in many cropping systems. The most efficient bacteria used for Sclerotinia Stem Rot management belonged mainly to the genera Bacillus [1,9,[12][13]21], Pseudomonas [7,22], Enterobacter [23,24], Serratia [22,[25][26][27], and at a lesser extent Streptomyces, Burkholderia, Pantoea, and Paenibacillus [22,25].…”

Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria

“…Biocontrol agents may seemingly represent an environmentally friendly alternative to potent and toxic fungicides, which cannot be broken down in the environment (Abdalla et al 2014). There are many studies on the biological control of S. sclerotiorum by using fungal (Krutova 1987;Hannusch & Boland 1995;Mischke et al 1995;McLaren et al 1996;Huang et al 2000;Pieckenstain et al 2001;Zhang et al 2004;Huang & Erickson 2007) and bacterial (Godoy et al 1990;Yuen et al 1991Yuen et al , 1994Expert & Digat 1995;Boland 1997;Tozlu 2003;Fernando et al 2004;Abdullah et al 2008) biocontrol agents in either laboratory or field-trials. However, many field studies failed to consistently control this pathogen due to the fact that Sclerotinia ascospores can disperse for a long distance and then even a reduced number of sclerotia in field can cause significant infection and yield loss (Venette 1998;Hedke et al 1999).…”

New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum

“…Some isolates from this study (T. koningiopsis, T. harzinaum and T. spirale) completely inhibited the production of Ss sclerotia. In the same sense, Abdullah et al (2008) observed inhibition of the formation of Ss sclerotia by T. harzianum, that decreased from 31.66 (treatment control) to 12.07 and 18.12 sclerotia. On the other hand, the most inhibitory isolate from the work reported by Amin et al (2010) …”

“…According to the Brazilian Ministry of Agriculture Livestock and Food Supply (MAPA) database (Agrofit 2016), there are five commercial products recommended for Ss control, formulated with either T. asperellum and T. harzianum. In order to select isolates for new formulations, some studies have reported the effectiveness of different species of Trichoderma in control of Ss, showing both inhibition of mycelial growth (Amin et al 2010, Samuel et al 2010, Matroudi et al 2009) and the parasitism of sclerotia (Abdullah et al 2008).…”