Biological identifications of mayflies (Ephemeroptera) using DNA barcodes

Ball, Shelley L.; Hebert, Paul D. N.; Burian, Steven K.; Webb, J. M.

doi:10.1899/04-142.1

Cited by 261 publications

(193 citation statements)

References 23 publications

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“…It will be required to perform thorough morphological studies to describe the valid species. The NJ tree supported the results of previous studies that have found the COI barcode to be an effective tool for the identification in mayflies (Ball et al 2005;Zhou et al 2010).…”

supporting

confidence: 86%

“…DNA barcodes have also implied in studying the systematics, diversity, ecology, biogeography, and conservation of aquatic insects Gattolliat et al 2015). A comprehensive barcode library has been established for mayflies from Canada, Mexico, and the United States (Ball et al 2005;Zhou et al 2009Zhou et al , 2010Webb et al 2012;Gattolliat et al 2015). To our knowledge, no molecular work of this kind was undertaken on mayflies in India so far.…”

mentioning

confidence: 99%

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DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

Selvakumar

Sivaramakrishnan²,

Janarthanan

2016

Mitochondrial DNA Part B

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In this study, DNA barcodes were generated for 40 species belonging to 32 genera under 10 families of Ephemeroptera from South India. Nucleotide sequence divergences were calculated using the Kimura two-parameter distance model and a neighbour-joining analysis was performed to provide a graphic display of the patterns of divergence among the species. This study demonstrates that COI barcoding is effective in discriminating among the mayfly species of South India, and provides a reference library for their future molecular identification.

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supporting

confidence: 86%

mentioning

confidence: 99%

DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

Selvakumar

Sivaramakrishnan²,

Janarthanan

2016

Mitochondrial DNA Part B

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“…is method has been shown to be e ective for the identi cation of a range of taxa including gastropods (Remigo and Hebert, 2003), springtails (Hogg and Hebert, 2004), butter ies (Hebert et al, 2004a), birds (Hebert et al, 2004b), may ies (Ball et al, 2005), sh (Ward et al, 2005), esh ies (Tan et al, 2010) and blow ies (Nelson et al, 2007;Tan et al, 2009).…”

mentioning

confidence: 99%

DNA barcoding for identification of mosquitoes (Diptera: Culicidae) from the Ryukyu Archipelago, Japan

Taira

Toma

Tamashiro

et al. 2012

Jap. J. Sanit. Zool.

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Abstract:e utility of cytochrome c oxidase subunit I (COI) DNA barcoding for the identi cation of 37 mosquito species belonging to the genera Anopheles (6 spp.), Aedes (14 spp.) and Culex (17 spp.) including subspecies from nine islands of the Ryukyu Archipelago, Japan was examined. A 658 bp fragment of the COI gene was sequenced from 228 specimens. Nucleotide sequence divergences were calculated using the Kimura-two parameter (K2P) distance model and a neighbor-joining (NJ) phylogenetic tree analysis was performed to provide a graphic display of the patterns in divergence among species. e mean intraspeci c variations of 35 species including the medically important vector species showed a less than 2 range with 0 1.5 divergences, but higher divergences of more than 2 were detected in Ae. aureostriatus okinawanus Bohart (2.2 ) and Cx. hayashii ryukyuanus Tanaka et al. (3.3 ). ey showed di erences in divergence between specimens from di erent islands. All 37 species were discriminated as distinct clusters in the NJ tree. e results in this study prove the utility of DNA barcoding for identi cation of mosquitoes from the Ryukyu Archipelago, Japan.

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“…Sequencing of standard regions of DNA, such as cox1 (e.g., Ball et al 2005), reduces the problems of assaying expressed loci and also reduces the variation in descriptive statistics by employing a standardised locus. A more important advantage is that using standardised data in each new study will facilitate a comparative analysis of all previous data.…”

Section: Dna Sequencesmentioning

confidence: 99%

“…The approach has been applied successfully to 64 species of mayfly in Madagascar (Monaghan et al 2009) with an important consequence being the ability to establish the necessary sample sizes of between 7 and 10 individuals per putative species (see also Morando et al 2003). Most studies using DNA sequence variation in taxonomy provide little justification of sample sizes employed (Ball et al 2005;Savolainen et al 2007;Webb et al 2007). While samples of some groups may be limited by their rarity in the field, it is important to consider how small sample sizes may affect any conclusions of group membership in the absence of additional data.…”

Section: Future Perspectives Taxonomymentioning

confidence: 99%

Genetic contributions to the study of taxonomy, ecology, and evolution of mayflies (Ephemeroptera): review and future perspectives

Monaghan

Sartori

2009

Aquatic Insects

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Since the late 1970s, allozymes, microsatellites, and DNA sequence variation have been widely used to study the taxonomy, ecology, and evolution of mayflies (Ephemeroptera). Early research investigated species limits in morphologically cryptic groups and attempted to link immature and adult stages using genetic markers. Population genetic studies soon followed, with a strong emphasis on measuring gene flow as a proxy for dispersal. Most recently, direct sequencing of DNA and molecular phylogenetic tools have broadened our understanding of mayfly systematics and evolution. Interestingly, these newer approaches have also begun to re-address some of the earliest questions about species circumscription and taxonomy. Here we present an overview of genetics in mayfly research to date, focusing on how genetic approaches have been applied to taxonomy, phylogenetics, and population genetics. We identify a number of outstanding questions and highlight some of the most interesting topics for future research. These include the need for evolutionarily valid species concepts when applying genetics to taxonomy, a better understanding of how parthenogenesis may affect population genetic structure, and increased use of DNA sequence data from nuclear gene regions.

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Biological identifications of mayflies (Ephemeroptera) using DNA barcodes

Cited by 261 publications

References 23 publications

DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

DNA barcoding for identification of mosquitoes (Diptera: Culicidae) from the Ryukyu Archipelago, Japan

Genetic contributions to the study of taxonomy, ecology, and evolution of mayflies (Ephemeroptera): review and future perspectives

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