Biological Studies on Cortisone in Mice

Transplantation from Man to Mouse of Exudates Containing Tumour Cells

“…However, by inhibiting the formation of new connective tissue (Ragan, Howes, Plotz, Meyer and Blunt, 1949;Spain, Molomut and Haber, 1950;Dorfman, 1953), the hormone will compromise the grafted tumour's supply of stroma needed for the proliferation of the malignant cells. It is the author's opinion that this difficulty might be overcome by injecting tumour cell containing exudates intraperitoneally.…”

“…One of the great difficulties found when attempting to obtain a successful take of heterologous tumours, is that the proliferating tumour cells must receive a supply of stroma from the animal host (Clemmesen, 1938), and the use of large doses of cortisone for the purpose of depressing the production of antibodies will restrain the formation of fibroblasts (Ragan, Howes, Plotz, Meyer and Blunt, 1949; Spain, Molomut and Haber, 1950;Baker and Whitaker, 1950), and thus increase the difficulty of obtaining growth of the grafted tumours. By the intraperitoneal transplantation of exudates containing tumour cells it is perhaps possible to overcome this problem, providing the inoculated cells can be kept in suspension.…”

Human Tumours in Cortisone-Treated Mice

“…Clemmesen in his monograph (1938) ascribes the growth of mouse tumours obtained in his experiments on roentgen irradiated rats largely to the use of tumours primarily poor in stroma. Furthermore, as cortisone is known to have a restrictive influence on the formation of connective tissue (Ragan, Howes, Plotz, Meyer and Blunt, 1949;Spain, Molomut and Haber, 1950;Cornman, 1951;Cavallero, Borasi, Sala and Amira, 1951;Taubenhaus, 1953), the possibilities of stroma formation, and thus of growth of the graft, will be very poor in animals treated with the hormone. Correspondingly in the author's experiments no proliferation of tumour cells was seen in solid grafts.…”

Effects of protease inhibitors on nuclear binding of glucocorticoid hormones in C3H10T½ cells

“…Although a wide variety of experimental conditions have been employed, the majority of results have reported suppressive effects of glucocorticoids on tumorigenesis [2,4,8-lo], the extent of such suppression being roughly parallel to the anti-inflammatory strength of the hormones [4,8,9] as well as to their ability to inhibit DNA synthesis 191. The relatively weak gluc~orticoid cortisone, however, was found to exhibit little or no suppression of tumorigenesis in many of the above systems [2,4,8], and cortisone has been shown in other studies to increase chemically-induced tumor incidences in rodents in a co-carcinogenic or promotor-like manner [1,3,. Our in vitro studies using the C3HIOT% cell line have found that cortisone both induces malignant transformation when added alone and enhances the yield of X-ray-induced transformation [1 11.…”